TOP TEN perturbations for NM_000389 (Homo sapiens)

Organism: Homo sapiens
Gene: NM_000389
Selected probe(set): 202284_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of NM_000389 (202284_s_at) across 5392 perturbations tested by GENEVESTIGATOR:

PMA; ionomycine study 2 / unstimulated CD4 memory T-cell sample

Relative Expression (log2-ratio):6.185399
Number of Samples:8 / 8
Experimental PMA; ionomycine study 2
CD4+ memory T cells derived from peripheral blood of healthy subjects were stimulated for 3 hours with phorbol 12-myristate 13-acetate (PMA, 10 ng/ml) and ionomycin (1 ?g/ml). ATC code:---
Control unstimulated CD4 memory T-cell sample
Unstimulated CD4+ memory T cells derived from peripheral blood of healthy subjects.

ARC study 1 / untreated MCF-7 cell sample

Relative Expression (log2-ratio):4.544959
Number of Samples:2 / 2
Experimental ARC study 1
MCF7 cells treated with 2uM ARC (4-amino-6-hydrazino-7-beta-d-ribofuranosyl-7H-pyrrolo-(2, 3-d)-pyrimidine-5-carboxamide, NSC 188491) for 24 hours. ATC code:---
Control untreated MCF-7 cell sample
MCF-7 cells untreated.

doxorubicin study 3 / untreated MCF-7 cell sample

Relative Expression (log2-ratio):4.5174723
Number of Samples:2 / 2
Experimental doxorubicin study 3
MCF7 cells treated with 2uM doxorubicin for 24 hours. ATC code:
Control untreated MCF-7 cell sample
MCF-7 cells untreated.

tumor supernatant activation study 3 / untreated memory CD4 T-cell sample

Relative Expression (log2-ratio):4.476124
Number of Samples:4 / 3
Experimental tumor supernatant activation study 3
Memory (CD45RA-) CD4+ T cells isolated from peripheral blood of female healthy donors were incubated for 24 hours in 1:1 mix of tumor supernatant from primary invasive breast ductal carcinoma and X-VIVO 20 medium supplemented with antiCD3/CD28 beads, before harvest for RNA isolation. The tumor supernatant was prepared as followed: fresh surgical specimen was dissociated in X-VIVO 20 medium by GentleMACS dissociator and the resulting suspension was clarified by centrifugation for 15min at 13'000 g. ATC code:---
Control untreated memory CD4 T-cell sample
Memory (CD45RA-) CD4+ T-cells were isolated from peripheral blood of healthy female donors and incubated in X-VIVO 20 medium for 24 hours, before harvest for RNA isolation.

B-CLL study 11 (DMSO) / vehicle (DMSO) treated normal B-cell sample

Relative Expression (log2-ratio):-4.071289
Number of Samples:4 / 4
Experimental B-CLL study 11 (DMSO)
Peripheral blood mononuclear cells (PBMCs) obtained from chronic lymphocytic leukemia (CLL) patients and in vitro treated with vehicle (DMSO) for 4 hours. PBMCs? samples contained 90% CD19+ CD5+ B lineage CLL cells (B-CLL). Inclusion criteria: untreated CLL patients or at least 1 month after chemotherapy. Exclusion criteria: patients with active infections or other serious medical conditions or with white blood cell (WBC) counts of 15,000/l.
Control vehicle (DMSO) treated normal B-cell sample
MACS purified resting B-cells from healthy donor peripheral blood treated with vehicle (DMSO) for 4 hours.

echinomycin study 1 / deferoxamine study 5

Relative Expression (log2-ratio):-4.0356283
Number of Samples:3 / 3
Experimental echinomycin study 1
Echinomycin (100nM; 2h) treated human astroglioma (U251) cells, stimulated with deferoxamine (DFO; 300mM; 16h). ATC code:---
Control deferoxamine study 5
Untreated human astroglioma (U251) cells, stimulated with deferoxamine (DFO; 300mM; 16h). ATC code:

adefovir study 1 (50uM) / vehicle (DMSO) treated HepG2 sample

Relative Expression (log2-ratio):4.020954
Number of Samples:3 / 21
Experimental adefovir study 1 (50uM)
HepG2 cells treated with compound: adefovir (50uM; CAS no.:106941-25-7) for 24 hours. Adefovir is non-hepatotoxic. HepG2 cells were treated with the IC20 concentration measured after 24 hours. ATC code:---
Control vehicle (DMSO) treated HepG2 sample
HepG2 cells treated with DMSO (0.5% v/v) as solvent control for 24 hours.

IFN-g study 11 / normal monocyte sample

Relative Expression (log2-ratio):3.908659
Number of Samples:7 / 7
Experimental IFN-g study 11
Monocyte samples isolated from donors and stimulated in vitro by 100 ng/ml IFN? in whole blood for 1.5 hour. Following stimulation, monocyte were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.
Control normal monocyte sample
Monocyte samples isolated from healthy subjects. Peripheral blood monocytes were isolated immediately after drawing blood by depletion from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.

succinate dehydrogenase B depletion study 1 (siRNA) / control siRNA transfected Hep3B cell sample

Relative Expression (log2-ratio):3.9073057
Number of Samples:3 / 3
Experimental succinate dehydrogenase B depletion study 1 (siRNA)
Hep3B cells stably transfected with short hairpin vectors containing an siRNA directed to the subunit B of the succinate dehydrogenase.
Control control siRNA transfected Hep3B cell sample
Hep3B cells stably transfected with empty siRNA expression vector (pU6).

p73 overexpr. study 1 (adenovirus) / control virus infected NCI-H1299 cell sample

Relative Expression (log2-ratio):3.883047
Number of Samples:2 / 2
Experimental p73 overexpr. study 1 (adenovirus)
NCI-H1299 cells infected with adenovirus expressing TAp73beta for 5 hours. Cells were harvested after 80% transduction efficiency was reached, as monitored by GFP fluorescence.
Control control virus infected NCI-H1299 cell sample
NCI-H1299 cells infected with control adenovirus expressing GFP for 5 hours. Cells were harvested after 80% transduction efficiency was reached, as monitored by GFP fluorescence.