TOP TEN perturbations for NP_722579 (Homo sapiens)

Organism: Homo sapiens
Gene: NP_722579
Selected probe(set): 229971_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of NP_722579 (229971_at) across 6593 perturbations tested by GENEVESTIGATOR:

Langerhans cell histiocytosis study 1 / normal plasmocytoid dendritic cell sample

Relative Expression (log2-ratio):-3.481369
Number of Samples:7 / 3
Experimental Langerhans cell histiocytosis study 1
Langerhans cell histiocytes (LCH) were isolated from LCH lesions of patients undergoing surgery. All patients had single system disease, five patients had bone lesion, one had skin lesion and one had mucosal manifestation. Langerhans cells histiocytes were purified by FACS as CD1a+ / CD270 + population with > 95% purity.
Control normal plasmocytoid dendritic cell sample
Normal plasmocytoid dendritic cells were isolated from peripheral blood of healthy adult donors. Plasmocytoid dendritic cells were defined as HLA-DR+ / CD45RAhi / CD11c- / BDCA2+ population.

acute respiratory distress syndrome study 1 / normal blood neutrophil sample

Relative Expression (log2-ratio):-2.5227957
Number of Samples:12 / 12
Experimental acute respiratory distress syndrome study 1
Primary neutrophils isolated from blood samples of patients fulfilling the Berlin criteria of acute respiratory distress syndrome (ARDS). Neutrophils were purified over discontinuous plasma-percoll gradients. Patients were representative of the full patients cohort in terms of age, gender, ARDS severity and causation.
Control normal blood neutrophil sample
Primary neutrophils isolated from blood samples of healthy volunteers purified over discontinuous plasma-percoll gradients.

DLBCL study 1 (activated B-cell like; unclassified) / DLBCL study 1 (activated B-cell like; memory-like)

Relative Expression (log2-ratio):-2.1150055
Number of Samples:11 / 3
Experimental DLBCL study 1 (activated B-cell like; unclassified)
Unclassified B-cells sorted from primary tumor samples from patients with malignant diffuse large B-cell lymphoma (activated B-cell like type). To isolate different B-cell subsets, tonsil tissues from DLBCL patients were sorted by fluorescence-activated cell sorting. Samples were also classified based on subset-specific B-cell-associated gene signature (BAGS).
Control DLBCL study 1 (activated B-cell like; memory-like)
Memory B-cells sorted from primary tumor samples from patients with malignant diffuse large B-cell lymphoma (activated B-cell like type). To isolate different B-cell subsets, tonsil tissues from DLBCL patients were sorted by fluorescence-activated cell sorting. Samples were also classified based on subset-specific B-cell-associated gene signature (BAGS).

NOTCH2-IC overexpr. study 1 (72h) / NOTCH1-IC overexpr. study 2 (72h)

Relative Expression (log2-ratio):-1.882844
Number of Samples:3 / 2
Experimental NOTCH2-IC overexpr. study 1 (72h)
EREB2-5 cell line stably transfected with expression plasmid coding for intracellular domain of NOTCH2 (NOTCH2-IC). The stably transfected cells were maintained in RPMI media and were induced immediately after estrogen withdrawal. The expression of NOTCH2-IC was induced by the addition of doxycycline to a final concentration of 100 ng/mL medium. Doxycycline induced cells were harvested 72 hours after induction. Estrogen withdrawal leads to the inactivation of EBNA2, resulting in cell cycle arrest. Before the preparation of RNA, NGFR cells were purified by MACS separation to enrich the cells with a transcriptional response to doxycycline.
Control NOTCH1-IC overexpr. study 2 (72h)
EREB2-5 cell line stably transfected with expression plasmid coding for intracellular domain of NOTCH1 (NOTCH1-IC). The stably transfected cells were maintained in RPMI media and were induced immediately after estrogen withdrawal. The expression of NOTCH1-IC was induced by the addition of doxycycline to a final concentration of 100 ng/mL medium. Doxycycline induced cells were harvested 72 hours after induction. Estrogen withdrawal leads to the inactivation of EBNA2, resulting in cell cycle arrest. Before the preparation of RNA, NGFR cells were purified by MACS separation to enrich the cells with a transcriptional response to doxycycline.

precursor-B-ALL study 3 (PAX5-rearranged) / precursor-B-ALL study 3 (MLL-rearranged)

Relative Expression (log2-ratio):-1.8285866
Number of Samples:6 / 5
Experimental precursor-B-ALL study 3 (PAX5-rearranged)
Peripheral blood and bone marrow samples of pediatric patients with precursor B-ALL [dic(9;20)(p11-13;q11) )/PAX5 rearranged]. Patients were part of the Nordic Society Of Pediatric Hematology And Oncology Group (NOPHO).
Control precursor-B-ALL study 3 (MLL-rearranged)
Peripheral blood and bone marrow samples of pediatric patients with precursor B-ALL [t(v;11q23)/MLL rearranged]. Patients were part of the Nordic Society Of Pediatric Hematology And Oncology Group (NOPHO).

DLBCL study 1 (activated B-cell like; plasmablast-like) / DLBCL study 1 (activated B-cell like; memory-like)

Relative Expression (log2-ratio):-1.8135405
Number of Samples:12 / 3
Experimental DLBCL study 1 (activated B-cell like; plasmablast-like)
Plasmablast B-cells sorted from primary tumor samples from patients with malignant diffuse large B-cell lymphoma (activated B-cell like type). To isolate different B-cell subsets, tonsil tissues from DLBCL patients were sorted by fluorescence-activated cell sorting. Samples were also classified based on subset-specific B-cell-associated gene signature (BAGS).
Control DLBCL study 1 (activated B-cell like; memory-like)
Memory B-cells sorted from primary tumor samples from patients with malignant diffuse large B-cell lymphoma (activated B-cell like type). To isolate different B-cell subsets, tonsil tissues from DLBCL patients were sorted by fluorescence-activated cell sorting. Samples were also classified based on subset-specific B-cell-associated gene signature (BAGS).

ovarian tumor study 16 / normal ovarian surface epithelial cell sample

Relative Expression (log2-ratio):1.807025
Number of Samples:3 / 5
Experimental ovarian tumor study 16
Human epithelial tumor cell samples from the ovary of patients with papillary serous carcinoma. Samples were derived by laser capture microdissection (LCM).
Control normal ovarian surface epithelial cell sample
Human epithelial cell samples from histopathological normal and non-cancerous ovary tissue.

B-CLL study 11 (rolipram) / rolipram study 4 (normal B-cell; 20uM)

Relative Expression (log2-ratio):-1.7799225
Number of Samples:4 / 4
Experimental B-CLL study 11 (rolipram)
Peripheral blood mononuclear cells (PBMCs) obtained from chronic lymphocytic leukemia (CLL) patients and in vitro treated with rolipram (20 uM, cyclic nucleotide phosphodiesterase (PDE4) inhibitor) for 4 hours. PBMCs’ samples contained 90% CD19+ CD5+ B lineage CLL cells (B-CLL). Inclusion criteria: untreated CLL patients or at least 1 month after chemotherapy. Exclusion criteria: patients with active infections or other serious medical conditions or with white blood cell (WBC) counts of 15,000/l. ATC code:---
Control rolipram study 4 (normal B-cell; 20uM)
MACS purified resting B-cells from healthy donor peripheral blood treated with rolipram (20 uM, cyclic nucleotide phosphodiesterase (PDE4) inhibitor) for 4 hours. ATC code:---

OPM-1 / MM1.S

Relative Expression (log2-ratio):-1.7213602
Number of Samples:4 / 4
Experimental OPM-1
Human primary cancer cell line derived from the peripheral blood of a patient with multiple myeloma. Dexamethasone-resistant. Synonyms:OPM1 Cellosaurus code:
Control MM1.S
Human primary cancer cell line derived from the peripheral blood of a patient with multiple myeloma. Dexamethasone-sensitive. Parental cell line:: MM.1 Synonyms:MM.1S; MM1-S; MM-1S; MM1S Cellosaurus code:

melanoma study 5 (persisting TIL) / melanoma study 5 (non-persisting TIL)

Relative Expression (log2-ratio):-1.7131453
Number of Samples:6 / 4
Experimental melanoma study 5 (persisting TIL)
Melanocyte-differentiation antigen (MDA; gp100154-162 or MART-126-35(27L) ) specific CD8+ T cell clones derived from HLA-A2+ patients with metastatic melanoma which showed persistence after adoptive T-cell transfer. Total RNA was isolated from pre-infusion CD8+ effector T-cells.
Control melanoma study 5 (non-persisting TIL)
Melanocyte-differentiation antigen (MDA; gp100154-162 or MART-126-35(27L) ) specific CD8+ T cell clones derived from HLA-A2+ patients with metastatic melanoma which did not persist after adoptive T-cell transfer. Total RNA was isolated from pre-infusion CD8+ effector T-cells.