TOP TEN perturbations for NP_766632 (Homo sapiens)

Organism: Homo sapiens
Gene: NP_766632
Selected probe(set): 222868_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of NP_766632 (222868_s_at) across 6593 perturbations tested by GENEVESTIGATOR:

IL-1b; IFN-g study 1 (132hrs) / untreated pancreatic islet sample (132hrs)

Relative Expression (log2-ratio):5.9379616
Number of Samples:2 / 2
Experimental IL-1b; IFN-g study 1 (132hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 132 hours. Media were changed after 2 and 5 days.
Control untreated pancreatic islet sample (132hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 132 hours. Media were changed after 2 and 5 days.

IL-1b; IFN-g study 1 (108hrs) / untreated pancreatic islet sample (108hrs)

Relative Expression (log2-ratio):5.5603523
Number of Samples:2 / 2
Experimental IL-1b; IFN-g study 1 (108hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 108 hours. Media were changed after 2 and 5 days.
Control untreated pancreatic islet sample (108hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 108 hours. Media were changed after 2 and 5 days.

IL-1b; IFN-g study 1 (120hrs) / untreated pancreatic islet sample (120hrs)

Relative Expression (log2-ratio):5.2975636
Number of Samples:2 / 2
Experimental IL-1b; IFN-g study 1 (120hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 120 hours. Media were changed after 2 and 5 days.
Control untreated pancreatic islet sample (120hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 120 hours. Media were changed after 2 and 5 days.

IL-1b; IFN-g study 1 (84hrs) / untreated pancreatic islet sample (84hrs)

Relative Expression (log2-ratio):5.1650534
Number of Samples:3 / 3
Experimental IL-1b; IFN-g study 1 (84hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 84 hours. Media were changed after 2 days.
Control untreated pancreatic islet sample (84hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 84 hours. Media were changed after 2 days.

IL-1b; IFN-g study 1 (72hrs) / untreated pancreatic islet sample (72hrs)

Relative Expression (log2-ratio):5.1389647
Number of Samples:3 / 3
Experimental IL-1b; IFN-g study 1 (72hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 72 hours. Media were changed after 2 days.
Control untreated pancreatic islet sample (72hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 72 hours. Media were changed after 2 days.

IL-1b; IFN-g study 1 (96hrs) / untreated pancreatic islet sample (96hrs)

Relative Expression (log2-ratio):5.0415993
Number of Samples:3 / 3
Experimental IL-1b; IFN-g study 1 (96hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 96 hours. Media were changed after 2 days.
Control untreated pancreatic islet sample (96hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 96 hours. Media were changed after 2 days.

IFN-g study 9 (24h) / untreated epidermal keratinocyte sample

Relative Expression (log2-ratio):4.9922314
Number of Samples:3 / 6
Experimental IFN-g study 9 (24h)
Primary keratinocytes treated with interferon gamma (IFN-g) for 24 hours. Monolayer normal human keratinocyte (NHK) cultures were established and used in the second or third passage. Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency. Cultures were starved of growth factors in nonsupplemented M154 medium for 24 hours, and afterwards stimulated with recombinant human IFN-g for 24 hours.
Control untreated epidermal keratinocyte sample
Untreated primary epidermal keratinocytes. Monolayer normal human keratinocyte (NHK) cultures were established and used in the second or third passage. Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.

IL-1b; IFN-g study 1 (60hrs) / untreated pancreatic islet sample (60hrs)

Relative Expression (log2-ratio):4.979289
Number of Samples:3 / 3
Experimental IL-1b; IFN-g study 1 (60hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 60 hours. Media were changed after 2 days.
Control untreated pancreatic islet sample (60hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 60 hours. Media were changed after 2 days.

IL-1b; IFN-g study 1 (48hrs) / untreated pancreatic islet sample (48hrs)

Relative Expression (log2-ratio):4.7436533
Number of Samples:3 / 3
Experimental IL-1b; IFN-g study 1 (48hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 48 hours.
Control untreated pancreatic islet sample (48hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 48 hours.

IL-1b; IFN-g study 1 (36hrs) / untreated pancreatic islet sample (36hrs)

Relative Expression (log2-ratio):4.7218437
Number of Samples:3 / 3
Experimental IL-1b; IFN-g study 1 (36hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 36 hours.
Control untreated pancreatic islet sample (36hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 36 hours.