TOP TEN perturbations for P05161 (Homo sapiens)

Organism: Homo sapiens
Gene: P05161
Selected probe(set): 205483_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of P05161 (205483_s_at) across 5610 perturbations tested by GENEVESTIGATOR:

interferon-alpha-A/D study 1 (late) / untreated BE(2)-C cell sample (mature)

Relative Expression (log2-ratio):7.1184273
Number of Samples:3 / 3
Experimental interferon-alpha-A/D study 1 (late)
Cultured BE(2)-C cells were differentiated with 10 uM all-trans retinoic acid (RA) for 3 weeks into cells with mature neuron cell characteristics and incubated with universal type I IFN (IFN-alpha-A/D) for 12 hours before samples were taken. ATC code:---
Control untreated BE(2)-C cell sample (mature)
Differentiated, cultured BE(2)-C neuroblastoma cells. Cells were differentiated with 10 uM all-trans retinoic acid (RA) for 3 weeks into cells with mature neuron cell characteristics before samples were taken.

interferon-alpha-A/D study 1 (early) / untreated BE(2)-C cell sample (mature)

Relative Expression (log2-ratio):6.9344845
Number of Samples:3 / 3
Experimental interferon-alpha-A/D study 1 (early)
Cultured BE(2)-C cells were differentiated with 10 uM all-trans retinoic acid (RA) for 3 weeks into cells with mature neuron cell characteristics and incubated with universal type I IFN (IFN-alpha-A/D) for 6 hours before samples were taken. ATC code:---
Control untreated BE(2)-C cell sample (mature)
Differentiated, cultured BE(2)-C neuroblastoma cells. Cells were differentiated with 10 uM all-trans retinoic acid (RA) for 3 weeks into cells with mature neuron cell characteristics before samples were taken.

interferon-alpha-A/D study 2 (late) / untreated BE(2)-C cell sample (immature)

Relative Expression (log2-ratio):6.626319
Number of Samples:3 / 3
Experimental interferon-alpha-A/D study 2 (late)
Undifferentiated, cultured BE(2)-C cells were incubated with universal type I IFN (IFNa-A/D) for 12 hours before samples were taken. ATC code:---
Control untreated BE(2)-C cell sample (immature)
Undifferentiated, cultured BE(2)-C neuroblastoma cell samples.

interferon-alpha-A/D study 2 (early) / untreated BE(2)-C cell sample (immature)

Relative Expression (log2-ratio):6.124503
Number of Samples:3 / 3
Experimental interferon-alpha-A/D study 2 (early)
Undifferentiated, cultured BE(2)-C cells were incubated with universal type I IFN (IFNa-A/D) for 6 hours before samples were taken. ATC code:---
Control untreated BE(2)-C cell sample (immature)
Undifferentiated, cultured BE(2)-C neuroblastoma cell samples.

smoking study 81 (CSE) / human rhinovirus study 3

Relative Expression (log2-ratio):-5.895052
Number of Samples:4 / 4
Experimental smoking study 81 (CSE)
Human bronchial epithelial (HBE) cells isolated from non-smoking individuals and subjected to a treatment with 50% cigarette smoke extract (CSE) for 24 hours. In order to prepare CSE, one reference cigarette 3R4F was bubbled into 4 ml of hydrocortisone-free bronchial epithelial cell growth medium (BEGM) for 5 minutes using a syringe apparatus. Absorbance of the obtained CSE was adjusted to 0.15 at 320 nm and the resulting CSE was used at 50% concentration. HBE cells were isolated by protease digestion of dissected airways and grown in BEGM. HBE cells were cultured in hydrocortisne-free BEGM overnight before the CSE stimulation. Cells were isolated from subjects that died from cerebrovascular causes or accidental head trauma, and had negative serologies for HIV1/2, HTLV1/2, hepatitis A, B and C, and syphilis.
Control human rhinovirus study 3
Human bronchial epithelial (HBE) cells isolated from non-smoking individuals and subjected to an infection with human rhinovirus 16 (HRV-16; MOI=1) for 24 hours. HBE cells were isolated by protease digestion of dissected airways and grown in BEGM. HBE cells were cultured in hydrocortisne-free BEGM overnight before the HRV-16 infection. Cells were isolated from subjects that died from cerebrovascular causes or accidental head trauma, and had negative serologies for HIV1/2, HTLV1/2, hepatitis A, B and C, and syphilis.

HIVGFP(G); SIVVLP(G) study 1 / uninfected monocyte-derived dendritic cell sample

Relative Expression (log2-ratio):5.7963285
Number of Samples:2 / 2
Experimental HIVGFP(G); SIVVLP(G) study 1
Monocyte-derived dendritic cell samples (MDDC) from isolated and stimulated CD14+ monocytes (GM-CSF and IL-4 for dendritic cell differentiation) of adult peripheral blood. MDDC were infected with GFP-encoding HIV-1 pseudotyped with VSV-G [HIVGFP(G)] and VSV-G-pseudotyped SIVmac239 virus-like particles [SIVVLP(G)] for 48 hours.
Control uninfected monocyte-derived dendritic cell sample
Monocyte-derived dendritic cell samples (MDDC) from isolated and stimulated CD14+ monocytes (GM-CSF and IL-4 for dendritic cell differentiation) of adult peripheral blood. MDDC were treated with lipopolysaccharide (LPS) for 48 hours.

p63 depletion study 1 (shRNA) / empty vector transduced ME180 cell sample

Relative Expression (log2-ratio):5.759674
Number of Samples:3 / 3
Experimental p63 depletion study 1 (shRNA)
ME180 cells were transduced with lentiviral supernatant containing pLL p63shRNA, and harvested ~65 hours later.
Control empty vector transduced ME180 cell sample
ME180 cells were transduced with lentiviral supernatant containing empty vector, and harvested ~65 hours later.

HIVGFP(G); SIVVLP(G) study 1 / HIVGFP(G) study 1

Relative Expression (log2-ratio):5.541029
Number of Samples:2 / 2
Experimental HIVGFP(G); SIVVLP(G) study 1
Monocyte-derived dendritic cell samples (MDDC) from isolated and stimulated CD14+ monocytes (GM-CSF and IL-4 for dendritic cell differentiation) of adult peripheral blood. MDDC were infected with GFP-encoding HIV-1 pseudotyped with VSV-G [HIVGFP(G)] and VSV-G-pseudotyped SIVmac239 virus-like particles [SIVVLP(G)] for 48 hours.
Control HIVGFP(G) study 1
Monocyte-derived dendritic cell samples (MDDC) from isolated and stimulated CD14+ monocytes (GM-CSF and IL-4 for dendritic cell differentiation) of adult peripheral blood. MDDC were infected with GFP-encoding HIV-1 pseudotyped with VSV-G [HIVGFP(G)] for 48 hours.

human rhinovirus study 3 / control bronchial epithelial cell sample

Relative Expression (log2-ratio):5.2565317
Number of Samples:4 / 4
Experimental human rhinovirus study 3
Human bronchial epithelial (HBE) cells isolated from non-smoking individuals and subjected to an infection with human rhinovirus 16 (HRV-16; MOI=1) for 24 hours. HBE cells were isolated by protease digestion of dissected airways and grown in BEGM. HBE cells were cultured in hydrocortisne-free BEGM overnight before the HRV-16 infection. Cells were isolated from subjects that died from cerebrovascular causes or accidental head trauma, and had negative serologies for HIV1/2, HTLV1/2, hepatitis A, B and C, and syphilis.
Control control bronchial epithelial cell sample
Human bronchial epithelial (HBE) cells isolated from non-smoking individuals and left untreated for 24 hours. HBE cells were isolated by protease digestion of dissected airways and grown in BEGM. HBE cells were cultured in hydrocortisne-free BEGM overnight and then for 24 hours. Cells were isolated from subjects that died from cerebrovascular causes or accidental head trauma, and had negative serologies for HIV1/2, HTLV1/2, hepatitis A, B and C, and syphilis.

dendritic cell study 7 (IL-4 mddc) / dendritic cell study 7 (IL-15 mddc)

Relative Expression (log2-ratio):-5.2168684
Number of Samples:3 / 3
Experimental dendritic cell study 7 (IL-4 mddc)
Mature IL-4 dendritic cells differentiated from CD14+ monocytes obtained from healthy donors. Monocytes were seeded in RPMI and supplemented with 2.5 % heat-inactivated human AB serum. Monocytes were cultured with 800 IU/mL granulocyte macrophage colony-stimulating factor and 20 ng/mL IL-4 in order to generate immature IL-4 DCs. Conventional maturation cocktail, comprising 10 ng/mL tumor necrosis factor-?, 10 ng/mL interleukin-1?, 15 ng/ml interleukin-6 and 1 ?g/mL prostaglandin E2 was added for 48 hours. CD14+ monocytes were isolated from peripheral blood mononuclear cells (PBMC) by Ficoll density gradient centrifugation and purified by positive magnetic cell selection.
Control dendritic cell study 7 (IL-15 mddc)
Mature IL-15 dendritic cells differentiated from CD14+ monocytes obtained from healthy donors. Monocytes were seeded in RPMI and supplemented with 2.5 % heat-inactivated human AB serum. Differentiation was induced with 800 IU/mL granulocyte macrophage colony-stimulating factor and 200 ng/mL IL-15 to obtain immature IL-15 DCs. A TLR-activating maturation cocktail, comprising 3 ?g/mL R848, 2.5 ng/mL tumor necrosis factor-?, 250 ng/mL interferon-? and 1 ?g/mL prostaglandin E2 was added after 24-48 hours of differentiation for 18-20 hours. CD14+ monocytes were isolated from peripheral blood mononuclear cells (PBMC) by Ficoll density gradient centrifugation and purified by positive magnetic cell selection.