TOP TEN perturbations for P05305 (Homo sapiens)

Organism: Homo sapiens
Gene: P05305
Selected probe(set): 222802_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of P05305 (222802_at) across 5392 perturbations tested by GENEVESTIGATOR:

E. coli study 2 / unstimulated, normal monocyte-derived macrophage sample

Relative Expression (log2-ratio):6.654191
Number of Samples:5 / 7
Experimental E. coli study 2
Monocyte-derived macrophage samples derived from healthy control subjects cultured for 4 hours with 2.5 ? 105 heat-killed E. coli.
Control unstimulated, normal monocyte-derived macrophage sample
Monocyte-derived macrophage samples derived from healthy control subjects cultured for 4 hours unstimulated.

A. fumigatus study 1 / uninfected immature dendritic cell sample

Relative Expression (log2-ratio):6.333497
Number of Samples:2 / 2
Experimental A. fumigatus study 1
5 x 106 immature dendritic cells were cultivated together with 5 x 106 A. fumigatus germ tubes for 6h until isolation of total RNA.
Control uninfected immature dendritic cell sample
5 x 106 immature dendritic cells were cultivated without fungi.

CD44s overexpr. study 1 / normal HEK-293 cell sample

Relative Expression (log2-ratio):6.101184
Number of Samples:3 / 3
Experimental CD44s overexpr. study 1
Human embryonic kidney cell line HEK-293 transfected with pcDNA3.1(-) vector containing codon-optimized human CD44 sequence for expression.
Control normal HEK-293 cell sample
Untransfected, native human embryonic kidney cell line HEK-293 cell samples.

sangivamycin study 1 / untreated MCF-7 cell sample

Relative Expression (log2-ratio):5.5273056
Number of Samples:2 / 2
Experimental sangivamycin study 1
MCF7 cells treated with 2uM sangivamycin for 24 hours. ATC code:---
Control untreated MCF-7 cell sample
MCF-7 cells untreated.

glioma study 16 (LN-229) / normal astrocyte sample

Relative Expression (log2-ratio):-5.4558372
Number of Samples:2 / 3
Experimental glioma study 16 (LN-229)
Human glioma cell line LN229 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37?C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

E. coli study 1 / unstimulated, normal monocyte-derived macrophage sample

Relative Expression (log2-ratio):5.3733177
Number of Samples:12 / 16
Experimental E. coli study 1
Monocyte-derived macrophage samples derived from healthy control subjects cultured for 4 hours with 2.5 ? 105 heat-killed E. coli.
Control unstimulated, normal monocyte-derived macrophage sample
Monocyte-derived macrophage samples derived from healthy control subjects cultured for 4 hours unstimulated.

peptidoglycan study 1 / mock treated neonatal neutrophils

Relative Expression (log2-ratio):4.7710295
Number of Samples:3 / 3
Experimental peptidoglycan study 1
Healthy neonatal neutrophils with ? 99% purity isolated from cord blood samples collected after normal, full-term, vaginal delivery treated with peptidoglycan isolated from S. aureus (10 ug/ml) for 4 hours.
Control mock treated neonatal neutrophils
Healthy neonatal neutrophils with ? 99% purity isolated from cord blood samples collected after normal, full-term, vaginal delivery mock treated for 4 hours.

actinomycin D study 1 / mannitol treated A549 cell sample

Relative Expression (log2-ratio):-4.744546
Number of Samples:3 / 3
Experimental actinomycin D study 1
A549 human lung cancer cells were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. ATC code:
Control mannitol treated A549 cell sample
A549 human lung cancer cells were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, mannitol (5% final concentration) was added to the culture.

glioma study 16 (BS-149) / normal astrocyte sample

Relative Expression (log2-ratio):-4.6423435
Number of Samples:2 / 3
Experimental glioma study 16 (BS-149)
Human glioma cell line BS149 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37?C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

acute coronary syndrome study 1 (STEMI) / peripheral blood leukocyte sample (STEMI)

Relative Expression (log2-ratio):4.405557
Number of Samples:4 / 4
Experimental acute coronary syndrome study 1 (STEMI)
Thrombus-derived leukocytes obtained from patients with ST-segment elevation myocardial infarction (STEMI) undergoing primary percutaneous coronary intervention (PCI). Leukocytes were derived from an occluding coronary thrombus, immediately after diagnostic angiography and identification of the culprit lesion, the presence of total coronary occlusion and angiographic signs of an occluding thrombus. The culprit coronary artery was intubated with a 6 F guiding catheter and wired with a 0.014 inch guide wire and prior to any subsequent interventional procedures, thrombi were removed from the site of coronary occlusion with an aspiration catheter and immersed in phosphate-buffered saline immediately.
Control peripheral blood leukocyte sample (STEMI)
Peripheral blood leukocytes derived from patients with ST-elevation myocardial infarction (STEMI).