TOP TEN perturbations for P09601 (Homo sapiens)

Organism: Homo sapiens
Gene: P09601
Selected probe(set): 203665_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of P09601 (203665_at) across 5610 perturbations tested by GENEVESTIGATOR:

tumor supernatant activation study 3 / untreated memory CD4 T-cell sample

Relative Expression (log2-ratio):6.997651
Number of Samples:4 / 3
Experimental tumor supernatant activation study 3
Memory (CD45RA-) CD4+ T cells isolated from peripheral blood of female healthy donors were incubated for 24 hours in 1:1 mix of tumor supernatant from primary invasive breast ductal carcinoma and X-VIVO 20 medium supplemented with antiCD3/CD28 beads, before harvest for RNA isolation. The tumor supernatant was prepared as followed: fresh surgical specimen was dissociated in X-VIVO 20 medium by GentleMACS dissociator and the resulting suspension was clarified by centrifugation for 15min at 13'000 g. ATC code:---
Control untreated memory CD4 T-cell sample
Memory (CD45RA-) CD4+ T-cells were isolated from peripheral blood of healthy female donors and incubated in X-VIVO 20 medium for 24 hours, before harvest for RNA isolation.

smoking study 80 (nasal epith; 3R4F; 4h; 0.15mg/l) / air exposed organotypic nasal epithelium culture sample (4h; 3R4F)

Relative Expression (log2-ratio):6.646758
Number of Samples:9 / 9
Experimental smoking study 80 (nasal epith; 3R4F; 4h; 0.15mg/l)
Organotypic human nasal epithelium culture 4 hours after exposure to diluted mainstream cigarette smoke (0.15 mg nicotine/l) from 3R4F reference cigarettes. Organotypic nasal MucilAirTM cultures were consisted of nasal epithelial cells derived from a healthy non-smoker 41-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The smoke was generated according to the Health Canada Intense smoking protocol (a 55-mL puff over 2s, twice per minute with an 8-s pump exhaust time). Each 3R4F cigarette was smoked to a defined standard butt length (approximately 35 mm), yielding approximately 10 – 11 puffs per stick. The cultures were exposed to smoke continuously for 28 minutes in smoking machine and 4 hours after smoke exposure the cells were collected for RNA extraction.
Control air exposed organotypic nasal epithelium culture sample (4h; 3R4F)
Organotypic human nasal epithelium culture 4 hours after exposure to conditioned fresh air. Organotypic nasal MucilAirTM cultures were consisted of nasal epithelial cells derived from a healthy non-smoker 41-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The cultures were exposed to conditioned fresh air continuously for 28 minutes. These samples represented control samples for 3R4F smoke exposure at timepoint 4 hours after exposure.

keratinocyte differentiation study 2 (KLF4 siRNA) / keratinocyte differentiation study 2

Relative Expression (log2-ratio):-6.6108665
Number of Samples:2 / 2
Experimental keratinocyte differentiation study 2 (KLF4 siRNA)
KLF4 (Kruppel-like factor 4) depleted primary neonatal keratinocytes differentiated with 1.2 mM calcium for 3 days. Keratinocytes obtained from freshly isolated foreskin were grown in keratinocyte-SFM medium supplemented with epidermal growth factor and bovine pituitary extract. KLF4 depletion was done using siRNAs: KLF4i(A): CCGAGGAGTTCAACGATCT; KLF4i(B): TGACCAGGCACTACCGTAA. Differentiation was induced at 100% confluency.
Control keratinocyte differentiation study 2
Primary neonatal keratinocytes differentiated with 1.2 mM calcium for 3 days. Keratinocytes obtained from freshly isolated foreskin were grown in keratinocyte-SFM medium supplemented with epidermal growth factor and bovine pituitary extract. Differentiation was induced at 100% confluency.

smoking study 58 (24 hrs) / smoking study 58 (4 hrs)

Relative Expression (log2-ratio):-6.528311
Number of Samples:3 / 3
Experimental smoking study 58 (24 hrs)
Nasal epithelium organotypic tissue culture harvested 24 hours (recovery phase) after 4 repetitive exposures to 10% (vol/vol) mainstream cigarette smoke (one 3R4F reference cigarette pro exposure), with 1 hour rest between each treatment. Exposure was performed directly at the air/liquid interface and culture medium was replaced immediately after treatment. The 3D tissue model was generated from primary human nasal epithelial cells derived from a 54 years old Caucasian non-smoking female donor co-cultured with primary human airway fibroblasts.
Control smoking study 58 (4 hrs)
Nasal epithelium organotypic tissue culture harvested 4 hours after 4 repetitive exposures to 10% (vol/vol) mainstream cigarette smoke (one 3R4F reference cigarette pro exposure), with 1 hour rest between each treatment. Exposure was performed directly at the air/liquid interface and culture medium was replaced immediately after treatment. The 3D tissue model was generated from primary human nasal epithelial cells derived from a 54 years old Caucasian non-smoking female donor co-cultured with primary human airway fibroblasts.

smoking study 80 (small airway epith; CHTP1.2; 4h; 0.4mg/l) / air exposed organotypic small airway epithelium culture sample (4h; CHTP1.2)

Relative Expression (log2-ratio):6.4661913
Number of Samples:6 / 6
Experimental smoking study 80 (small airway epith; CHTP1.2; 4h; 0.4mg/l)
Organotypic human small airway epithelium culture 4 hours after exposure to 0.4 mg nicotine/l aerosol from a potential modified-risk tobacco product, carbon-heated tobacco product 1.2 (CHTP1.2). Organotypic small airway SmallAirTM cultures were consisted of bronchiolar cells derived from a healthy non-smoker 55-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The aerosol was generated according to the Health Canada Intense smoking protocol (a 55-mL puff over 2s, twice per minute with an 8-s pump exhaust time). Each CHTP1.2 stick was used for a total of 12 puffs per stick. The cultures were exposed to aerosol continuously for 28 minutes in smoking machine and 4 hours after aerosol exposure the cells were collected for RNA extraction.
Control air exposed organotypic small airway epithelium culture sample (4h; CHTP1.2)
Organotypic human small airway epithelium culture 4 hours after exposure to conditioned fresh air. Organotypic small airway SmallAirTM cultures were consisted of bronchiolar cells derived from a healthy non-smoker 55-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The cultures were exposed to conditioned fresh air continuously for 28 minutes. These samples represented control samples for CHTP1.2 aerosol exposure (carbon-heated tobacco product 1.2) at timepoint 4 hours after exposure.

smoking study 80 (nasal epith; CHTP1.2; 4h; 0.51mg/l) / air exposed organotypic nasal epithelium culture sample (4h; CHTP1.2)

Relative Expression (log2-ratio):6.459096
Number of Samples:9 / 9
Experimental smoking study 80 (nasal epith; CHTP1.2; 4h; 0.51mg/l)
Organotypic human nasal epithelium culture 4 hours after exposure to 0.51 mg nicotine/l aerosol from a potential modified-risk tobacco product, carbon-heated tobacco product 1.2 (CHTP1.2). Organotypic nasal MucilAirTM cultures were consisted of nasal epithelial cells derived from a healthy non-smoker 41-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The aerosol was generated according to the Health Canada Intense smoking protocol (a 55-mL puff over 2s, twice per minute with an 8-s pump exhaust time). Each CHTP1.2 stick was used for a total of 12 puffs per stick. The cultures were exposed to aerosol continuously for 28 minutes in smoking machine and 4 hours after aerosol exposure the cells were collected for RNA extraction.
Control air exposed organotypic nasal epithelium culture sample (4h; CHTP1.2)
Organotypic human nasal epithelium culture 4 hours after exposure to conditioned fresh air. Organotypic nasal MucilAirTM cultures were consisted of nasal epithelial cells derived from a healthy non-smoker 41-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The cultures were exposed to conditioned fresh air continuously for 28 minutes. These samples represented control samples for CHTP1.2 aerosol exposure (carbon-heated tobacco product 1.2) at timepoint 4 hours after exposure.

smoking study 80 (small airway epith; CHTP1.2; 4h; 0.3mg/l) / air exposed organotypic small airway epithelium culture sample (4h; CHTP1.2)

Relative Expression (log2-ratio):6.4204645
Number of Samples:6 / 6
Experimental smoking study 80 (small airway epith; CHTP1.2; 4h; 0.3mg/l)
Organotypic human small airway epithelium culture 4 hours after exposure to 0.3 mg nicotine/l aerosol from a potential modified-risk tobacco product, carbon-heated tobacco product 1.2 (CHTP1.2). Organotypic small airway SmallAirTM cultures were consisted of bronchiolar cells derived from a healthy non-smoker 55-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The aerosol was generated according to the Health Canada Intense smoking protocol (a 55-mL puff over 2s, twice per minute with an 8-s pump exhaust time). Each CHTP1.2 stick was used for a total of 12 puffs per stick. The cultures were exposed to aerosol continuously for 28 minutes in smoking machine and 4 hours after aerosol exposure the cells were collected for RNA extraction.
Control air exposed organotypic small airway epithelium culture sample (4h; CHTP1.2)
Organotypic human small airway epithelium culture 4 hours after exposure to conditioned fresh air. Organotypic small airway SmallAirTM cultures were consisted of bronchiolar cells derived from a healthy non-smoker 55-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The cultures were exposed to conditioned fresh air continuously for 28 minutes. These samples represented control samples for CHTP1.2 aerosol exposure (carbon-heated tobacco product 1.2) at timepoint 4 hours after exposure.

smoking study 5 light (intermediate) / air exposed bronchial epithelial cell sample

Relative Expression (log2-ratio):6.2531996
Number of Samples:8 / 7
Experimental smoking study 5 light (intermediate)
Normal human bronchial epithelial cells exposed to 15min FTC smoking of a typical American brand of "light" cigarettes with each 35mL puff diluted into 500cc air. Following exposure the medium was replaced with fresh and the cells placed in a 37°C incubator for 4h or 8h.
Control air exposed bronchial epithelial cell sample
Normal human bronchial epithelial cells exposed to air for 15 min. Following exposure the medium was replaced with fresh and the cells placed in a 37°C incubator for 4h or 8h.

smoking study 6 brand cig. (intermediate) / air exposed bronchial epithelial cell sample

Relative Expression (log2-ratio):6.208811
Number of Samples:3 / 3
Experimental smoking study 6 brand cig. (intermediate)
Normal human bronchial epithelial cells exposed to smoke from a typical "full flavor" American brand of cigarettes for 15 min. Afterwards cells were re-fed with fresh media and incubated for 4h.
Control air exposed bronchial epithelial cell sample
Normal human bronchial epithelial cells exposed to air for 15 min. Afterwards cells were fed with fresh media and incubated for 4h.

smoking study 5 reference (intermediate) / air exposed bronchial epithelial cell sample

Relative Expression (log2-ratio):6.1370745
Number of Samples:8 / 7
Experimental smoking study 5 reference (intermediate)
Normal human bronchial epithelial cells exposed to 15min FTC smoking of a reference cigarette (2R4F, University of Kentucky) with each 35mL puff diluted into 500cc air. Following exposure the medium was replaced with fresh and the cells placed in a 37°C incubator for 4h or 8h.
Control air exposed bronchial epithelial cell sample
Normal human bronchial epithelial cells exposed to air for 15 min. Following exposure the medium was replaced with fresh and the cells placed in a 37°C incubator for 4h or 8h.