TOP TEN perturbations for P0C0S8 (Homo sapiens)

Organism: Homo sapiens
Gene: P0C0S8
Selected probe(set): 208583_x_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of P0C0S8 (208583_x_at) across 5217 perturbations tested by GENEVESTIGATOR:

HIVGFP(G); SIVVLP(G) study 1 / uninfected monocyte-derived dendritic cell sample

Relative Expression (log2-ratio):1.8683872
Number of Samples:2 / 2
Experimental HIVGFP(G); SIVVLP(G) study 1
Monocyte-derived dendritic cell samples (MDDC) from isolated and stimulated CD14+ monocytes (GM-CSF and IL-4 for dendritic cell differentiation) of adult peripheral blood. MDDC were infected with GFP-encoding HIV-1 pseudotyped with VSV-G [HIVGFP(G)] and VSV-G-pseudotyped SIVmac239 virus-like particles [SIVVLP(G)] for 48 hours.
Control uninfected monocyte-derived dendritic cell sample
Monocyte-derived dendritic cell samples (MDDC) from isolated and stimulated CD14+ monocytes (GM-CSF and IL-4 for dendritic cell differentiation) of adult peripheral blood. MDDC were treated with lipopolysaccharide (LPS) for 48 hours.

tumor supernatant activation study 3 / untreated memory CD4 T-cell sample

Relative Expression (log2-ratio):1.8671694
Number of Samples:4 / 3
Experimental tumor supernatant activation study 3
Memory (CD45RA-) CD4+ T cells isolated from peripheral blood of female healthy donors were incubated for 24 hours in 1:1 mix of tumor supernatant from primary invasive breast ductal carcinoma and X-VIVO 20 medium supplemented with antiCD3/CD28 beads, before harvest for RNA isolation. The tumor supernatant was prepared as followed: fresh surgical specimen was dissociated in X-VIVO 20 medium by GentleMACS dissociator and the resulting suspension was clarified by centrifugation for 15min at 13'000 g. ATC code:---
Control untreated memory CD4 T-cell sample
Memory (CD45RA-) CD4+ T-cells were isolated from peripheral blood of healthy female donors and incubated in X-VIVO 20 medium for 24 hours, before harvest for RNA isolation.

fluc-mrfp-ttk overexpr. study 1 (lentivirus; high) / control virus infected mesenchymal stem cell sample

Relative Expression (log2-ratio):1.8642731
Number of Samples:2 / 2
Experimental fluc-mrfp-ttk overexpr. study 1 (lentivirus; high)
FACS sorted fraction of human mesenchymal stem cells that were transduced with lentivirus containing the luciferase, mono red fluorescence protein and thymidine kinase gene triple vector (fluc-mrfp-ttk, MOI 16) and incubated for 36 hours. Sorted cell fraction with an intensity of MGFP >10^3 (high expression).
Control control virus infected mesenchymal stem cell sample
Human mesenchymal stem cells were transduced with lentivirus containing only the backbone of the virus without the triple vector.

LPS study 4 (shRNA cycT1) / cycT1 depletion study 2 (shRNA)

Relative Expression (log2-ratio):1.833374
Number of Samples:2 / 2
Experimental LPS study 4 (shRNA cycT1)
MONO-MAC-6 (MM6) cells were transduced with shRNA against cyclin T1 and then treated with 10 ng/ml lipopolysaccharide (LPS). ATC code:---
Control cycT1 depletion study 2 (shRNA)
MONO-MAC-6 (MM6) cells were transduced with shRNA against cyclin T1 and then mock treated.

colchicine study 7 (4000uM) / vehicle (medium) treated hepatocyte sample

Relative Expression (log2-ratio):1.8207455
Number of Samples:2 / 2
Experimental colchicine study 7 (4000uM)
Hepatocytes treated with compound: colchicine (4000uM; CHEMBL107) for 24 hours. ATC code:
Control vehicle (medium) treated hepatocyte sample
Hepatocytes treated with vehicle (medium) for 24 hours.

zalypsis study 2 / untreated OPM1 cell sample

Relative Expression (log2-ratio):1.8158951
Number of Samples:2 / 2
Experimental zalypsis study 2
OPM1 multiple myeloma cells treated in vitro with zalypsis (5 nM), a novel marine-derived compound with potent antimyeloma activity. Cells were harvested at the beginning of induction of cell death (15-20% cell death as assessed by Annexin V-FITC staining). ATC code:---
Control untreated OPM1 cell sample
OPM1 multiple myeloma cells untreated.

tumor supernatant activation study 3 / memory T-cell activation study 1

Relative Expression (log2-ratio):1.7812147
Number of Samples:4 / 3
Experimental tumor supernatant activation study 3
Memory (CD45RA-) CD4+ T cells isolated from peripheral blood of female healthy donors were incubated for 24 hours in 1:1 mix of tumor supernatant from primary invasive breast ductal carcinoma and X-VIVO 20 medium supplemented with antiCD3/CD28 beads, before harvest for RNA isolation. The tumor supernatant was prepared as followed: fresh surgical specimen was dissociated in X-VIVO 20 medium by GentleMACS dissociator and the resulting suspension was clarified by centrifugation for 15min at 13'000 g. ATC code:---
Control memory T-cell activation study 1
Memory (CD45RA-) CD4+ T-cells isolated from peripheral blood of healthy female donors were activated with anti-CD3/CD28 beads for 24hrs.

adrenal gland cancer study 4 / normal adrenal cortex tissue

Relative Expression (log2-ratio):1.7321787
Number of Samples:43 / 4
Experimental adrenal gland cancer study 4
Primary tumor tissue samples of the adrenal cortex from patients with adrenocortical carcinoma (ACC).
Control normal adrenal cortex tissue
Histological normal adrenal gland cortical tissue samples derived after autopsy.

formaldehyde study 3 (4500ug/ml) / vehicle (EtOH) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):1.7030411
Number of Samples:3 / 3
Experimental formaldehyde study 3 (4500ug/ml)
Bronchial epithelial cells (NHBE) treated with 4500 ug/ml formaldehyde (within range of concentrations reported to induce toxicity in lung epithelial cells and other cell types) for 24 hours. NHBE cells were derived from a 60 year old male non-smoker. ATC code:---
Control vehicle (EtOH) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (ethanol) at a final concentration of 2% v/v (concentration that ensures >80% cell viability after 24 hours of exposure) for 24 hours. NHBE cells were derived from a 60 year old male non-smoker.

HIVGFP(G); SIVVLP(G) study 1 / SIVVLP(G) study 1

Relative Expression (log2-ratio):1.6938686
Number of Samples:2 / 2
Experimental HIVGFP(G); SIVVLP(G) study 1
Monocyte-derived dendritic cell samples (MDDC) from isolated and stimulated CD14+ monocytes (GM-CSF and IL-4 for dendritic cell differentiation) of adult peripheral blood. MDDC were infected with GFP-encoding HIV-1 pseudotyped with VSV-G [HIVGFP(G)] and VSV-G-pseudotyped SIVmac239 virus-like particles [SIVVLP(G)] for 48 hours.
Control SIVVLP(G) study 1
Monocyte-derived dendritic cell samples (MDDC) from isolated and stimulated CD14+ monocytes (GM-CSF and IL-4 for dendritic cell differentiation) of adult peripheral blood. MDDC were infected with VSV-G-pseudotyped SIVmac239 virus-like particles [SIVVLP(G)] for 48 hours.