TOP TEN perturbations for P14543 (Homo sapiens)

Organism: Homo sapiens
Gene: P14543
Selected probe(set): 202007_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of P14543 (202007_at) across 5610 perturbations tested by GENEVESTIGATOR:

HCC study 20 (CDX; Hep-G2; ectopic) / HCC study 20 (PDX; ectopic)

Relative Expression (log2-ratio):-8.512192
Number of Samples:3 / 11
Experimental HCC study 20 (CDX; Hep-G2; ectopic)
Tumor tissue biopsy samples from Hep-G2 cell line-derived xenograft (CDX) ectopically generated in SCID mice by injecting hepatocellular carcinoma cells subcutaneously. In order to establish ectopic models, cells were injected subcutaneously into the flank regions. The mice were euthanized after two weeks.
Control HCC study 20 (PDX; ectopic)
Tumor tissue biopsy samples from patient-derived xenograft (PDX) sample ectopically generated in SCID mice by injecting hepatocellular carcinoma cells subcutaneously. Donor tumor tissue was obtained intraoperatively during liver resection from three patients. All three patients had hepatocellular carcinoma confirmed by histology. In order to establish ectopic models, cells were injected subcutaneously into the flank regions. The mice were euthanized after two weeks.

HCC study 20 (CDX; Hep-G2; orthotopic) / HCC study 20 (PDX; orthotopic)

Relative Expression (log2-ratio):-8.332922
Number of Samples:5 / 11
Experimental HCC study 20 (CDX; Hep-G2; orthotopic)
Tumor tissue biopsy samples from Hep-G2 cell line-derived xenograft (CDX) orthotopically generated in SCID mice by injecting hepatocellular carcinoma cells directly into liver parenchyma. In order to establish orthotopic model, the left lobe of liver was exposed through midline abdominal incision and cells were injected directly into liver parenchyma of mice. The mice were euthanized after two weeks.
Control HCC study 20 (PDX; orthotopic)
Tumor tissue biopsy samples from patient-derived xenograft (PDX) sample orthotopically generated in SCID mice by injecting hepatocellular carcinoma cells directly into liver parenchyma. Donor tumor tissue was obtained intraoperatively during liver resection from three patients. All three patients had hepatocellular carcinoma confirmed by histology. In order to establish orthotopic model, the left lobe of liver was exposed through midline abdominal incision and cells were injected directly into liver parenchyma of mice. The mice were euthanized after two weeks.

renal cell carcinoma study 6 (chromophobe type) / normal kidney tissue

Relative Expression (log2-ratio):-5.0181646
Number of Samples:4 / 2
Experimental renal cell carcinoma study 6 (chromophobe type)
Tumor tissue samples from the kidney of patients with chromophobe renal cell carcinoma (cRCC).
Control normal kidney tissue
Normal fetal kidney tissue samples.

prostate cancer study 8 (p. canc) / prostate cancer study 8 (ptasc)

Relative Expression (log2-ratio):-4.7512426
Number of Samples:3 / 2
Experimental prostate cancer study 8 (p. canc)
CD26+ FACS sorted prostate neoplasm cell (p. canc) samples from patients with primary prostate cancer collected after radical prostatectomy.
Control prostate cancer study 8 (ptasc)
CD90+ FACS sorted prostate tumor-associated stromal cell (ptasc) samples from patients with primary prostate cancer collected after radical prostatectomy.

ethyl methanesulfonate study 6 (4mM) / untreated TK6 cell sample

Relative Expression (log2-ratio):4.5202703
Number of Samples:3 / 15
Experimental ethyl methanesulfonate study 6 (4mM)
TK6 cells treated with 4mM ethyl methanesulfonate for 24h. ATC code:---
Control untreated TK6 cell sample
Untreated TK6 cell line. Cells where left untreated for 24h before harvesting.

methyl methanesulfonate study 6 (320µM) / untreated TK6 cell sample

Relative Expression (log2-ratio):4.486471
Number of Samples:3 / 15
Experimental methyl methanesulfonate study 6 (320µM)
TK6 cells treated with 320µM methyl methanesulfonate for 24h. ATC code:---
Control untreated TK6 cell sample
Untreated TK6 cell line. Cells where left untreated for 24h before harvesting.

prostate cancer study 8 (p. canc) / prostate cancer study 8 (psfmc)

Relative Expression (log2-ratio):-4.3923254
Number of Samples:3 / 5
Experimental prostate cancer study 8 (p. canc)
CD26+ FACS sorted prostate neoplasm cell (p. canc) samples from patients with primary prostate cancer collected after radical prostatectomy.
Control prostate cancer study 8 (psfmc)
CD49a+ FACS sorted prostate stromal fibromuscular cell (psfmc) samples from patients with primary prostate cancer collected after radical prostatectomy.

RhoA overexpr. study 1 (G14V) / RhoA overexpr. study 1 (WT)

Relative Expression (log2-ratio):4.1198745
Number of Samples:3 / 3
Experimental RhoA overexpr. study 1 (G14V)
Human mammary epithelial cells (hMEC) immortalized by retroviral transfection with a constitutively active RhoA mutant (G14V). Cells were cultivated in DFCI-I medium with 100ug/ml of G418.
Control RhoA overexpr. study 1 (WT)
Human mammary epithelial cells (hMEC) immortalized by retroviral transfection with wild-type RhoA. Cells were cultivated in DFCI-I medium with 100ug/ml of G418.

formaldehyde study 9 (200µM) / untreated TK6 cell sample

Relative Expression (log2-ratio):4.1004095
Number of Samples:3 / 15
Experimental formaldehyde study 9 (200µM)
TK6 cells treated with 200µM formaldehyde for 24h. ATC code:---
Control untreated TK6 cell sample
Untreated TK6 cell line. Cells where left untreated for 24h before harvesting.

EWS/FLI-1 depletion study 3 (FLI-1 siRNA) / mock transfected SK-N-MC cell sample

Relative Expression (log2-ratio):4.0375605
Number of Samples:4 / 2
Experimental EWS/FLI-1 depletion study 3 (FLI-1 siRNA)
Neuroepitheliomal SK-N-MC cell tranfected with 10nM FLI-1 siRNA to knock down the EWS/FLI-1 fusion within the transfected cell line. Another identical transfection was done 24 hours later and samples were taken 48 hours after the first transfection.
Control mock transfected SK-N-MC cell sample
Neuroepitheliomal SK-N-MC cells treated with transfection agent for 48 hours.