TOP TEN perturbations for P31941 (Homo sapiens)

Organism: Homo sapiens
Gene: P31941
Selected probe(set): 210873_x_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of P31941 (210873_x_at) across 5392 perturbations tested by GENEVESTIGATOR:

IL-4; GM-CSF study 1 (intermediate) / untreated monocyte sample

Relative Expression (log2-ratio):-6.0370064
Number of Samples:7 / 12
Experimental IL-4; GM-CSF study 1 (intermediate)
Monocytes, cultured with vehicle (DMSO/ethanol) and 500 U/ml IL-4 and 800 U/ml GM-CSF for 24 hours.
Control untreated monocyte sample
Freshly isolated human monocytes from healthy donors.

PMA study 6 (500ng/ml) / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):6.031913
Number of Samples:3 / 17
Experimental PMA study 6 (500ng/ml)
Bronchial epithelial cells (NHBE) treated with tetradecanoylphorbol acetate (500ng/ml; vendor: Sigma / catalog number: P1585 / catalog name: Phorbol 12-myristate 13-acetate [16561-29-8]) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial). ATC code:---
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).

skin transplantation study 1 (3d) / normal skin tissue (skin grafting)

Relative Expression (log2-ratio):5.9912863
Number of Samples:6 / 8
Experimental skin transplantation study 1 (3d)
Human skin punch biopsy from patients after skin grafting. Sample was taken at the site of re-epithelialization 3 days after surgery.
Control normal skin tissue (skin grafting)
Skin biopsy samples from healthy control subjects after breast or abdomen reduction surgery.

IL-4; GM-CSF study 1 (late) / untreated monocyte sample

Relative Expression (log2-ratio):-5.9065065
Number of Samples:6 / 12
Experimental IL-4; GM-CSF study 1 (late)
Monocytes, cultured with vehicle (DMSO/ethanol) and 500 U/ml IL-4 and 800 U/ml GM-CSF for 5 days. Cytokine treatment was repeated at day 3.
Control untreated monocyte sample
Freshly isolated human monocytes from healthy donors.

IL-4 / GM-CSF study 2 / differentiated (IL-4/GM-CSF) monocyte derived dendritic cell sample

Relative Expression (log2-ratio):5.842044
Number of Samples:5 / 5
Experimental IL-4 / GM-CSF study 2
Monocyte derived dendritic cells isolated after 5 days of IL4 / GM-CSF induced differentiation in the presence of airway epithelial cells (16HBE 14o).
Control differentiated (IL-4/GM-CSF) monocyte derived dendritic cell sample
Monocyte derived dendritic cells isolated after 5 days of IL4 / GM-CSF induced differentiation in the absence of airway epithelial cells (16HBE 14o).

skin transplantation study 1 (3d) / split-thickness skin grafting study 1

Relative Expression (log2-ratio):5.5295696
Number of Samples:6 / 6
Experimental skin transplantation study 1 (3d)
Human skin punch biopsy from patients after skin grafting. Sample was taken at the site of re-epithelialization 3 days after surgery.
Control split-thickness skin grafting study 1
Skin punch biopsy from patients with skin injury (burn injury, congelation, wound, compartment syndrome) immediately after split-thickness skin grafting was done. Sample was taken from the acute wound after grafting.

split-thickness skin grafting study 2 (NPWT) / normal skin tissue (skin grafting)

Relative Expression (log2-ratio):5.300892
Number of Samples:4 / 6
Experimental split-thickness skin grafting study 2 (NPWT)
Skin punch biopsy from patients that underwent split-thickness skin grafting. Sample was taken from the wound site 7 days after grafting. Patients received negative-pressure wound therapy (NPWT) immediately after grafting .
Control normal skin tissue (skin grafting)
Skin punch biopsy samples from healthy patients before slit-thickness skin grafting.

diphenylcyclopropenone study 1 (late) / diphenylcyclopropenone study 1 (early)

Relative Expression (log2-ratio):-4.9985285
Number of Samples:6 / 10
Experimental diphenylcyclopropenone study 1 (late)
Skin biopsy samples of healthy volunteers exposed to diphenylcyclopropenone (diphencyprone; DPCP) for 4 to 8 months. ATC code:---
Control diphenylcyclopropenone study 1 (early)
Skin biopsy samples of healthy volunteers exposed to diphenylcyclopropenone (diphencyprone; DPCP) for 3 days. ATC code:---

conditioned medium study 1 (HS27a) / untreated monocyte (CD14+) sample

Relative Expression (log2-ratio):-4.9849396
Number of Samples:2 / 2
Experimental conditioned medium study 1 (HS27a)
CD14+ monocytes treated with HS27a conditioned medium (CM) for 48h.
Control untreated monocyte (CD14+) sample
Untreated CD14+ monocytes from two different donors.

IFN-a2b study 1 / untreated immature dendritic cell sample

Relative Expression (log2-ratio):4.9449883
Number of Samples:3 / 3
Experimental IFN-a2b study 1
Immature dendritic cells (DCs) treated with 1000 U/ml of IFN-a2b.
Control untreated immature dendritic cell sample
Immature dendritic cells (DCs) without IFN alpha treatment.