TOP TEN perturbations for Q05655 (Homo sapiens)

Organism: Homo sapiens
Gene: Q05655
Selected probe(set): 202545_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of Q05655 (202545_at) across 5610 perturbations tested by GENEVESTIGATOR:

glioma study 16 (LN-229) / normal astrocyte sample

Relative Expression (log2-ratio):3.4716635
Number of Samples:2 / 3
Experimental glioma study 16 (LN-229)
Human glioma cell line LN229 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37?C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

renal cell carcinoma study 6 (chromophobe type) / normal kidney tissue

Relative Expression (log2-ratio):2.800558
Number of Samples:4 / 2
Experimental renal cell carcinoma study 6 (chromophobe type)
Tumor tissue samples from the kidney of patients with chromophobe renal cell carcinoma (cRCC).
Control normal kidney tissue
Normal fetal kidney tissue samples.

colorectal cancer study 4 (recurring) / adjacent colon tissue (recurring)

Relative Expression (log2-ratio):-2.77135
Number of Samples:24 / 3
Experimental colorectal cancer study 4 (recurring)
LCM-tumor tissue samples of patients who received resection after diagnosis of primary colorectal cancer. Patients developed metastatic recurrence during follow-up after surgery.
Control adjacent colon tissue (recurring)
Histologically normal colon tissue samples from patients with primary colorectal cancer collected after resection. Tissue was further extracted using laser-capture microdissection (LCM). With metastatic recurrence after resection during follow-up.

precursor-B-ALL study 1 (t(12;21)(p12,q22)) / normal bone marrow sample

Relative Expression (log2-ratio):-2.605754
Number of Samples:58 / 74
Experimental precursor-B-ALL study 1 (t(12;21)(p12,q22))
Bone marrow samples of patients with precursor B-ALL (t(12;21)(p12,q22)/TEL-AML1).
Control normal bone marrow sample
Non-leukemic and healthy bone marrow sample.

colorectal cancer study 4 (non-recurring) / adjacent colon tissue (non-recurring)

Relative Expression (log2-ratio):-2.5922832
Number of Samples:76 / 18
Experimental colorectal cancer study 4 (non-recurring)
LCM-tumor tissue samples of patients who received resection after diagnosis of primary colorectal cancer. Patients did not develop metastatic recurrence during follow-up after surgery.
Control adjacent colon tissue (non-recurring)
Histologically normal colon tissue samples from patients with primary colorectal cancer collected after resection. Tissue was further extracted using laser-capture microdissection (LCM). No metastatic recurrence during follow-up.

T-ALL study 1 / normal bone marrow sample

Relative Expression (log2-ratio):-2.5774784
Number of Samples:174 / 74
Experimental T-ALL study 1
Bone marrow samples of patients with T-ALL.
Control normal bone marrow sample
Non-leukemic and healthy bone marrow sample.

glioma study 17 (glioblastoma; unsorted) / non-tumor cortical tissue

Relative Expression (log2-ratio):2.52987
Number of Samples:2 / 4
Experimental glioma study 17 (glioblastoma; unsorted)
Brain cells isolated from high grade glioblastoma (grade IV). Tumor tissue was dissociated using enzymatic treatment and all cells were used for RNA isolation.
Control non-tumor cortical tissue
Cortical tissue obtained from patients with epilepsy, but without any manifested brain cancer. The tissue was dissociated using enzymatic treatment, but all brain cell types were used for analyses.

sangivamycin study 1 / untreated MCF-7 cell sample

Relative Expression (log2-ratio):-2.4841099
Number of Samples:2 / 2
Experimental sangivamycin study 1
MCF7 cells treated with 2uM sangivamycin for 24 hours. ATC code:---
Control untreated MCF-7 cell sample
MCF-7 cells untreated.

TNF-? study 17 (4h) / osteoarthritis study 6

Relative Expression (log2-ratio):2.4080992
Number of Samples:3 / 6
Experimental TNF-? study 17 (4h)
Synovial fibroblast samples isolated from affected knee of patients with osteoarthritis (OA) were stimulated by 10 ng/ml of TNF-? in serum-free DMEM for 4 hours. Average duration of OA: 4.4 ? 0.6 years, all patients were rheumatoid factor negative. Patients were receiving non-steroidal antiinflammatory drugs. CRP: 13.2 ? 2.9 mg/l. Synovial fibroblasts were isolated from surgically obtained synovial membrane by trypsin/collagenase digestion, followed by negative purification using CD-14 Dynabeads? M-450. Before the TNF-? stimulation, fibroblasts were cultured for four passages in DMEM medium supplemented with antibiotics and 10% FCS.
Control osteoarthritis study 6
Synovial fibroblast samples isolated from affected knee of patients with osteoarthritis (OA). Average duration of OA: 4.4 ? 0.6 years, all patients were rheumatoid factor negative. Patients were receiving non-steroidal antiinflammatory drugs. CRP: 13.2 ? 2.9 mg/l. Synovial fibroblasts were isolated from surgically obtained synovial membrane by trypsin/collagenase digestion, followed by negative purification using CD-14 Dynabeads? M-450. The fibroblasts were than cultured for four passages in DMEM medium supplemented with antibiotics and 10% FCS. Prior RNA isolation, the fibroblasts were washed in serum free DMEM.

breast cancer study 8 (metastatic) / normal breast tissue

Relative Expression (log2-ratio):-2.3409557
Number of Samples:2 / 4
Experimental breast cancer study 8 (metastatic)
Human metastatic infiltrating ductal carcinoma sample of the breast derived from lymph nodes of a female patient with breast cancer.
Control normal breast tissue
Normal human breast samples of healthy female individuals.