TOP TEN perturbations for Q14002 (Homo sapiens)

Organism: Homo sapiens
Gene: Q14002
Selected probe(set): 206199_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of Q14002 (206199_at) across 5880 perturbations tested by GENEVESTIGATOR:

esophageal squamous cell carcinoma study 1 / normal esophageal epithelium sample

Relative Expression (log2-ratio):-8.171836
Number of Samples:8 / 18
Experimental esophageal squamous cell carcinoma study 1
Esophageal squamous cell carcinoma (ESCC) biopsy samples from chemotherapy-naive patients with histological grading G1 (well differentiated) and UICC stage II and III, which undergone esophagectomy.
Control normal esophageal epithelium sample
Histologically normal esophageal squamous cell epithelium biopsy samples from patients that were investigated for esophageal pain, but diagnosed as healthy.

pancreatic cancer study 9 (PDX; infiltrating duct carcinoma, NOS; metastatic) / pancreatic cancer study 9 (PDX; infiltrating duct carcinoma, NOS; unstated status)

Relative Expression (log2-ratio):-5.580035
Number of Samples:4 / 7
Experimental pancreatic cancer study 9 (PDX; infiltrating duct carcinoma, NOS; metastatic)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from a metastasis of patients with primary infiltrating duct carcinoma, NOS of the pancreas (subcutaneously implanted). Metastatic site of patient tumor sample is not reported.
Control pancreatic cancer study 9 (PDX; infiltrating duct carcinoma, NOS; unstated status)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with infiltrating duct carcinoma, NOS of the pancreas (subcutaneously implanted). Primary site of the patient tumor sample is not reported.

Barrett's esophagus study 3 / esophageal squamous cell carcinoma study 1

Relative Expression (log2-ratio):5.0641737
Number of Samples:17 / 8
Experimental Barrett's esophagus study 3
Esophageal epithelium samples from areas with Barrett's esophagus metaplasia which were recovered by laser capture microdissection.
Control esophageal squamous cell carcinoma study 1
Esophageal squamous cell carcinoma (ESCC) biopsy samples from chemotherapy-naive patients with histological grading G1 (well differentiated) and UICC stage II and III, which undergone esophagectomy.

diabetes type 2 study 27 (LCM) / diabetes type 2 study 27 (enzymatic)

Relative Expression (log2-ratio):-4.8045387
Number of Samples:34 / 19
Experimental diabetes type 2 study 27 (LCM)
Pancreatic islet samples obtained from type 2 diabetic (T2D) phenotyped pancreatectomized patients (PPP) and isolated by laser capture microdissection (LCM). Islets specimens were retrieved by LCM from snap-frozen surgical specimen from patients who underwent pancreatectomy for pancreatic diseases. Histopathology of the resected tissue did not reveal insulitis in any PPP. Patients age <18 years were excluded. Patients with type 2 diabetes had fasting glycemia ≥7.0 mmol/l; HbA1C ≥6.5% and history of diabetes for >1 year.
Control diabetes type 2 study 27 (enzymatic)
Pancreatic islet samples obtained from type 2 diabetic (T2D) patients and isolated by enzymatic digestion. Well-preserved islets were isolated by collagenase digestion of pancreas from brain-dead organ donors which suffered from type 2 diabetes. After 2±1 days of culture, islets were successfully hand-picked and processed for further analyses. Type 2 diabetes was diagnosed based on clinical history, treatment with glucose-lowering drugs, and lack of anti-GAD65 autoantibodies.

colorectal cancer study 4 (recurring) / adjacent colon tissue (recurring)

Relative Expression (log2-ratio):-4.793208
Number of Samples:24 / 3
Experimental colorectal cancer study 4 (recurring)
LCM-tumor tissue samples of patients who received resection after diagnosis of primary colorectal cancer. Patients developed metastatic recurrence during follow-up after surgery.
Control adjacent colon tissue (recurring)
Histologically normal colon tissue samples from patients with primary colorectal cancer collected after resection. Tissue was further extracted using laser-capture microdissection (LCM). With metastatic recurrence after resection during follow-up.

colorectal cancer study 13 (tvvad) / normal colon tissue

Relative Expression (log2-ratio):-4.7869577
Number of Samples:3 / 3
Experimental colorectal cancer study 13 (tvvad)
Colon biopsies derived from patients with low grade tubulovillous/villous adenoma.
Control normal colon tissue
Colon biopsies derived from healthy controls.

pancreatic islet study 3 (expanded; NIH) / normal pancreatic islet sample

Relative Expression (log2-ratio):-4.777332
Number of Samples:3 / 7
Experimental pancreatic islet study 3 (expanded; NIH)
Pancreatic islet cells were expanded according to National Institutes of Health (NIH) protocol for 10 weeks. Expansion phase: 2,000 islet equivalents enriched by retention on a 40-μm filter were seeded onto tissue culture–treated dishes in CMRL-1066 medium containing 2 mmol/l l-glutamine and 10% fetal bovine serum.
Control normal pancreatic islet sample
Pancreatic islets were obtained from seven donors aged between 37 and 70 years and body mass index between 22 and 27. Functional islets were cultured in CMRL 1066 supplemented with 10% FCS, 1% glutamine, 5.6 mM glucose, 1 mM HEPES, 110 U/ml penicillin, and 110 g/ml streptomycin for 7 days or immediately processed after isolation.

pancreatic islet study 3 (expanded; PPRF) / normal pancreatic islet sample

Relative Expression (log2-ratio):-4.7511463
Number of Samples:2 / 7
Experimental pancreatic islet study 3 (expanded; PPRF)
Human pancreatic islets were isolated from a cadaveric donor. The population was 70% pure in mature islets, which was determined by dithizone staining. Islets cells were expanded according to Pharmaceutical Production Research Facility Protocol (PPRF) for 6 weeks. Expansion phase: Approximately 2,500 islet equivalents were seeded onto 75-cm2 fibronectin-coated tissue culture-treated flasks in a proprietary serum-free medium (PPRF medium). The serum-free PPRF medium was supplemented with 30% serum-free mesenchymal-conditioned medium. When the cell migration resulted in the formation of large-size colonies, the cells were harvested by trypsinization and replated into new tissue culture flasks. Once the cells reached near confluence (80–90%) in monolayer, they were trypsinized, counted, and subcultured at a density of 5,000 cells per cm2. Thereafter, cells were serially passaged using the same protocol.
Control normal pancreatic islet sample
Pancreatic islets were obtained from seven donors aged between 37 and 70 years and body mass index between 22 and 27. Functional islets were cultured in CMRL 1066 supplemented with 10% FCS, 1% glutamine, 5.6 mM glucose, 1 mM HEPES, 110 U/ml penicillin, and 110 g/ml streptomycin for 7 days or immediately processed after isolation.

colorectal cancer study 30 / normal colorectal tissue

Relative Expression (log2-ratio):-4.5943804
Number of Samples:45 / 4
Experimental colorectal cancer study 30
Formalin-fixed-paraffin-embedded (FFPE) colorectal tissue samples from patients with colorectal cancer.
Control normal colorectal tissue
Healthy, non-tumor colorectal tissue.

ulcerative colitis study 11 / colorectal cancer study 13 (tvvad)

Relative Expression (log2-ratio):4.5877104
Number of Samples:3 / 3
Experimental ulcerative colitis study 11
Colon biopsies derived from patients with ulcerative colitis.
Control colorectal cancer study 13 (tvvad)
Colon biopsies derived from patients with low grade tubulovillous/villous adenoma.