TOP TEN perturbations for Q5D1E8 (Homo sapiens)

Organism: Homo sapiens
Gene: Q5D1E8
Selected probe(set): 218810_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of Q5D1E8 (218810_at) across 5339 perturbations tested by GENEVESTIGATOR:

E. coli study 2 / unstimulated, normal monocyte-derived macrophage sample

Relative Expression (log2-ratio):4.6867523
Number of Samples:5 / 7
Experimental E. coli study 2
Monocyte-derived macrophage samples derived from healthy control subjects cultured for 4 hours with 2.5 ? 105 heat-killed E. coli.
Control unstimulated, normal monocyte-derived macrophage sample
Monocyte-derived macrophage samples derived from healthy control subjects cultured for 4 hours unstimulated.

breast cancer study 3 / normal organelle sample

Relative Expression (log2-ratio):-4.4388943
Number of Samples:17 / 12
Experimental breast cancer study 3
Breast basal tumor samples.
Control normal organelle sample
Normal organelle samples.

IFN-g; LPS study 1 / normal resting monocyte sample

Relative Expression (log2-ratio):4.0196695
Number of Samples:2 / 2
Experimental IFN-g; LPS study 1
Monocytes LPS/IFN-g activated for 30h.
Control normal resting monocyte sample
Monocytes resting for 30h.

breast cancer study 5 / normal organelle sample

Relative Expression (log2-ratio):-3.9072342
Number of Samples:20 / 12
Experimental breast cancer study 5
Breast non-BLC (sporadic basal-like cancer) tumor samples.
Control normal organelle sample
Normal organelle samples.

Merkel cell carcinoma study 3 (primary) / skin squamous cell carcinoma study 6

Relative Expression (log2-ratio):-3.811798
Number of Samples:38 / 4
Experimental Merkel cell carcinoma study 3 (primary)
Primary tumor tissue from the skin of patients with Merkel cell carcinoma.
Control skin squamous cell carcinoma study 6
Primary tumor tissue from the skin of patients with squamous cell carcinoma (SCC).

breast cancer study 4 / normal organelle sample

Relative Expression (log2-ratio):-3.8116074
Number of Samples:4 / 12
Experimental breast cancer study 4
Breast BRCA1-deficient tumor samples.
Control normal organelle sample
Normal organelle samples.

rheumatoid arthritis study 31 / TNF-? study 20

Relative Expression (log2-ratio):-3.8014727
Number of Samples:4 / 3
Experimental rheumatoid arthritis study 31
Monocyte samples isolated from patients with rheumatoid arthritis (RA). Monocyte from whole blood were depleted from granulocytes by CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling. Patients fulfilled the revised American College of Rheumatology criteria (ACR) for RA.
Control TNF-? study 20
Monocyte samples isolated from healthy donors and stimulated in vitro by 100 ng/ml TNF? in whole blood for 1.5 hour. Following stimulation, monocyte were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.

nutlin-3 (10uM); flagellin (500 ng/ml) study 1 (control vector) / nutlin-3 study 2 (control vector; 10uM)

Relative Expression (log2-ratio):3.7157402
Number of Samples:6 / 6
Experimental nutlin-3 (10uM); flagellin (500 ng/ml) study 1 (control vector)
Human breast adenocarcinoma cells MCF-7 expressing wild type p53, stably transfected with control empty vector pSUPER, treated with nutlin-3 (10 uM) for 45 hours followed by an additional 3 hours treatment of nutlin-3 (10uM) and flagellin (500 ng/ml). Cells were cultured in RPMI-1640 medium supplemented with 10% heat inactivated FBS and 50 U/ml of penicillin, 50 ug/ml of streptomycin. ATC code:---
Control nutlin-3 study 2 (control vector; 10uM)
Human breast adenocarcinoma cells MCF-7 expressing wild type p53, stably transfected with control empty vector pSUPER, treated with nutlin-3 (10 uM) for 48 hours. Cells were cultured in RPMI-1640 medium supplemented with 10% heat inactivated FBS and 50 U/ml of penicillin, 50 ug/ml of streptomycin. ATC code:---

P. gingivalis study 1 (fimbriae) / saline treated macrophage sample

Relative Expression (log2-ratio):3.7095776
Number of Samples:3 / 3
Experimental P. gingivalis study 1 (fimbriae)
Macrophage samples isolated from healthy blood donors treated with P. gingivalis fimbriae for 2 hours.
Control saline treated macrophage sample
Macrophage samples isolated from healthy blood donors treated with saline for 2 hours.

P. gingivalis study 1 (live) / saline treated macrophage sample

Relative Expression (log2-ratio):3.6463842
Number of Samples:3 / 3
Experimental P. gingivalis study 1 (live)
Macrophage samples isolated from healthy blood donors infected with live P. gingivalis 381 for 2 hours.
Control saline treated macrophage sample
Macrophage samples isolated from healthy blood donors treated with saline for 2 hours.