TOP TEN perturbations for Q86UK0 (Homo sapiens)

Organism: Homo sapiens
Gene: Q86UK0
Selected probe(set): 215465_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of Q86UK0 (215465_at) across 5638 perturbations tested by GENEVESTIGATOR:

TGF-β study 11 / untreated HCC827 cell sample

Relative Expression (log2-ratio):-4.8159165
Number of Samples:3 / 3
Experimental TGF-β study 11
HCC827 cells were cultured for 3-5 weeks in the presence of 2ng/ml of TGF-β, to induce epithelial-mesenchymal transition (EMT). 24 hours prior analysis cells were re-seeded without TGF-β.
Control untreated HCC827 cell sample
HCC827 cells grown in standard media.

HIF-1a/HIF-2a depletion study 1 (hypoxia; HK2) / HIF-1a/HIF-2a depletion study 1 (normoxia; AB81)

Relative Expression (log2-ratio):-4.5876102
Number of Samples:3 / 3
Experimental HIF-1a/HIF-2a depletion study 1 (hypoxia; HK2)
Proximal tubule epithelial cell line HK-2 with shRNA-mediated stable knock-down of HIF-1a and HIF-2a (Hypoxia-inducible factor 1-alpha and Hypoxia-inducible factor 2-alpha) 24 hours after treatment with hypoxia (20% O2). HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.
Control HIF-1a/HIF-2a depletion study 1 (normoxia; AB81)
Human podocyte cells AB81 with shRNA-mediated stable knock-down of HIF-1a and HIF-2a (Hypoxia-inducible factor 1-alpha and Hypoxia-inducible factor 2-alpha) 24 hours after treatment with normoxia (20% O2). Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates.

Hep-G2 / HepaRG

Relative Expression (log2-ratio):-4.0178437
Number of Samples:9 / 12
Experimental Hep-G2
Human primary cancer cell line derived from the liver of a patient with hepatocellular carcinoma. Synonyms:HEP-G2; Hep G2; HEP G2; HepG2; HEPG2 Cellosaurus code:
Control HepaRG
Immortalized cancer cell line derived from female patient with hepatocellular carcinoma. Cells can be induced to differentiate into hepatocyte-like cells by exposure to DMSO. Synonyms:Hepa-RG Cellosaurus code:

Merkel cell carcinoma study 3 (metastatic) / normal skin tissue

Relative Expression (log2-ratio):-3.9225006
Number of Samples:22 / 64
Experimental Merkel cell carcinoma study 3 (metastatic)
Metastatic tumor tissue from different metastatic sites (skin, lymph node, parotid gland) of patients with Merkel cell carcinoma of the skin.
Control normal skin tissue
Normal skin samples from healthy donors.

hepatocyte (ESC) / HepaRG

Relative Expression (log2-ratio):-3.7970886
Number of Samples:8 / 12
Experimental hepatocyte (ESC)
Hepatocyte-like cells differentiated from embryonic stem cells (ESC)
Control HepaRG
Immortalized cancer cell line derived from female patient with hepatocellular carcinoma. Cells can be induced to differentiate into hepatocyte-like cells by exposure to DMSO. Synonyms:Hepa-RG Cellosaurus code:

Merkel cell carcinoma study 3 (primary) / normal skin tissue

Relative Expression (log2-ratio):-3.6519556
Number of Samples:38 / 64
Experimental Merkel cell carcinoma study 3 (primary)
Primary tumor tissue from the skin of patients with Merkel cell carcinoma.
Control normal skin tissue
Normal skin samples from healthy donors.

Roberts syndrome study 1 / normal lymphoblastoid cell line sample

Relative Expression (log2-ratio):3.6069784
Number of Samples:2 / 18
Experimental Roberts syndrome study 1
Lymphoblastoid cell line samples from probands with Roberts syndrome (RBS).
Control normal lymphoblastoid cell line sample
Lymphoblastoid cell line samples from healthy subjects.

hereditary gingival fibromatosis study 1 / normal gingiva tissue

Relative Expression (log2-ratio):3.3915482
Number of Samples:2 / 2
Experimental hereditary gingival fibromatosis study 1
Hereditary gingival fibromatosis (HGF) patient gingiva tissue.
Control normal gingiva tissue
Normal gingiva tissue.

gefitinib study 2 / wild type A431 cell sample

Relative Expression (log2-ratio):-3.3798285
Number of Samples:3 / 3
Experimental gefitinib study 2
Gefitinib-resistant A431 (A431 GR) cells generated by growing parental cells in the presence of increasing concentrations of gefitinib up to 3 μM. Cells were selected and maintained in gefitinib (3μM). ATC code:
Control wild type A431 cell sample
A431 wild type cells.

aflatoxin B1 study 2 (2.5 uM; HepaRG) / vehicle (DMSO) treated HepaRG cell sample

Relative Expression (log2-ratio):3.3026571
Number of Samples:3 / 12
Experimental aflatoxin B1 study 2 (2.5 uM; HepaRG)
HepaRG cells exposed to 2.5 uM aflatoxin B1 (dissolved in 0.5% v/v DMSO) for 72 hours. Cells were cultivated in DMSO-containing medium between days 13 - 19 to allow them to differentiate into hepatocyte-like cells and become fully functional. Chemical was added at day 19 of cultivation. Cells were treated with the IC10 determined by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test (number of replicates, n = 3). ATC code:---
Control vehicle (DMSO) treated HepaRG cell sample
HepaRG cells treated with vehicle (DMSO 0.5% v/v) for 72 hours. Firstly, cells were cultivated in DMSO-containing medium between days 13 - 19 to allow them to differentiate into hepatocyte-like cells and become fully functional. Then the vehicle was added.