TOP TEN perturbations for Q8IZF2 (Homo sapiens)

Organism: Homo sapiens
Gene: Q8IZF2
Selected probe(set): 212950_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of Q8IZF2 (212950_at) across 5392 perturbations tested by GENEVESTIGATOR:

glioma study 16 (BS-149) / normal astrocyte sample

Relative Expression (log2-ratio):-5.960415
Number of Samples:2 / 3
Experimental glioma study 16 (BS-149)
Human glioma cell line BS149 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37?C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

glioma study 16 (LN-319) / normal astrocyte sample

Relative Expression (log2-ratio):-5.839328
Number of Samples:2 / 3
Experimental glioma study 16 (LN-319)
Human glioma cell line LN319 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37?C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

glioma study 16 (LN-229) / normal astrocyte sample

Relative Expression (log2-ratio):-5.822419
Number of Samples:2 / 3
Experimental glioma study 16 (LN-229)
Human glioma cell line LN229 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37?C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

SDF study 4 / untreated MDA Mb231 cell sample

Relative Expression (log2-ratio):-5.753484
Number of Samples:3 / 6
Experimental SDF study 4
MDA Mb231 cells treated with 75nM stromal derived factor (SDF; Cxcl12) for 6h.
Control untreated MDA Mb231 cell sample
Untreated MDA Mb231 cells harvested after 6h.

glioma study 16 (LN-215) / normal astrocyte sample

Relative Expression (log2-ratio):-5.03016
Number of Samples:2 / 3
Experimental glioma study 16 (LN-215)
Human glioma cell line LN215 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37?C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

oxyphilic adenoma study 1 / normal kidney tissue

Relative Expression (log2-ratio):4.735859
Number of Samples:4 / 2
Experimental oxyphilic adenoma study 1
Tumor tissue samples from the kidney of patients with oxyphilic adenoma (renal oncocytoma).
Control normal kidney tissue
Normal fetal kidney tissue samples.

renal cell carcinoma study 5 (papillary type) / normal kidney tissue

Relative Expression (log2-ratio):-4.3014965
Number of Samples:19 / 3
Experimental renal cell carcinoma study 5 (papillary type)
Tumor tissue samples from the kidney of patients with papillary renal cell carcinoma (pRCC).
Control normal kidney tissue
Normal adult kidney tissue samples.

glioma study 16 (LN-18) / normal astrocyte sample

Relative Expression (log2-ratio):-4.031494
Number of Samples:2 / 3
Experimental glioma study 16 (LN-18)
Human glioma cell line LN018 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

smoking study 31 / smoking study 30

Relative Expression (log2-ratio):3.8939562
Number of Samples:9 / 18
Experimental smoking study 31
Small airway epithelial cells from healthy smokers, derived by fiberoptic bronchoscopy with conscious sedation. After mild sedation with meperidine and midazolam and routine anesthesia of the vocal cords and bronchial airways with topical lidocaine was achieved, the fiberoptic bronchoscope was positioned proximal to the opening of a desired lobar bronchus. A 2 mm diameter brush was advanced approximately 7 to 10 cm distal from the third order bronchial branch and the end of the brush was wedged at about the 10th to 12th generation branch of the right lower lobe. Small airway epithelial cells were collected by gently gliding the brush back and forth on the epithelium 5 to 10 times in 8 to 10 different locations in the same general area.
Control smoking study 30
Tracheal epithelial cells from healthy smokers. derived by tracheal brushing without conscious sedation. Tracheal epithelial cells were collected with a fiberoptic bronchoscope .Atropine 0.6 mg (IM) and 2.5 mL, 0.02% ipratropium was nebulized to decrease salivary and bronchial secretions. Topical anesthesia of the posterior pharynx and vocal cords was obtained by inhalation with 2 mL, 4% lidocaine followed by administering 1 spray of 20% benzocaine to the posterior pharynx. Unless there was a contraindication, the bronchoscope was introduced through the nostril after application of viscous 2% lidocaine with the patient sitting upright. Once the bronchoscope passed to the posterior pharynx, further topical anesthesia of the vocal cords and trachea was obtained with 1 mL aliquots of 1% lidocaine through the working port of the bronchoscope (maximum amount of lidocaine: 5 mg/kg body weight). After adequate topical anesthesia was obtained, a 2 mm cytology brush was advanced through the working channel of the bronchoscope and tracheal epithelial cells were obtained by gently gliding the brush back and forth 20 times on tracheal epithelium in at least five different locations.

basal cell carcinoma study 3 / normal epidermal keratinocytes

Relative Expression (log2-ratio):3.8938885
Number of Samples:4 / 2
Experimental basal cell carcinoma study 3
Primary tumor tissue from the eyelid of patients with basal cell carcinoma (BCC).
Control normal epidermal keratinocytes
Normal human epidermal keratinocytes (NHEK) (Kurabo Ind., Ltd., Osaka, Japan) cultured in HuMedia-KB2 medium at 37?C in humidified air containing 5% CO2.