TOP TEN perturbations for Q8N2U0 (Homo sapiens)

Organism: Homo sapiens
Gene: Q8N2U0
Selected probe(set): 227063_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of Q8N2U0 (227063_at) across 5654 perturbations tested by GENEVESTIGATOR:

glioma study 16 (LN-18) / normal astrocyte sample

Relative Expression (log2-ratio):2.7789793
Number of Samples:2 / 3
Experimental glioma study 16 (LN-18)
Human glioma cell line LN018 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

azathioprine study 8 (48h) / vehicle (DMSO) treated HepG2 cell sample

Relative Expression (log2-ratio):-2.1685772
Number of Samples:2 / 19
Experimental azathioprine study 8 (48h)
HepG2 cells exposed to 250μM azathioprine in DMSO solvent for 48 hours. ATC code:
Control vehicle (DMSO) treated HepG2 cell sample
HepG2 cells exposed to DMSO solvent for 48 hours.

sirolimus study 6 (light) / sirolimus study 6 (heavy)

Relative Expression (log2-ratio):2.0828695
Number of Samples:3 / 3
Experimental sirolimus study 6 (light)
Human fetal lung fibroblast cell line (MRC5) treated for 50 minutes with sirolimus (100 nM; vendor: LC laboratories / catalog name: rapamycin). Cells in the growing phase (at 60% confluency) were hypertonically shocked by changing the condition media to a 300 mM NaCl containing Medium for 30 minutes. After hypertonic shock cells were transferred back to isotonic conditions for additional 30 min. Rapamycin was added to the cells during hypertonic shock until the end of recovery (50 minutes total exposure). After treatment RNA was isolated from the light polysome fraction. ATC code:
Control sirolimus study 6 (heavy)
Human fetal lung fibroblast cell line (MRC5) treated for 50 minutes with sirolimus (100 nM; vendor: LC laboratories / catalog name: rapamycin). Cells in the growing phase (at 60% confluency) were hypertonically shocked by changing the condition media to a 300 mM NaCl containing Medium for 30 minutes. After hypertonic shock cells were transferred back to isotonic conditions for additional 30 min. Rapamycin was added to the cells during hypertonic shock until the end of recovery (50 minutes total exposure). After treatment RNA was isolated from the heavy polysome fraction. ATC code:

DN-GRHL2 overexpression study 2 (D7) / control EGFP transfected bronchial epithelial cell sample (D7)

Relative Expression (log2-ratio):-2.02592
Number of Samples:3 / 3
Experimental DN-GRHL2 overexpression study 2 (D7)
Primary human bronchial epithelial (HBE) cells from donors without pre-existing lung disease infected with lentivirus containing a dominant negative mutant form of grainyheadlike family member 2 (DN-GRHL2). HBE cells (passage 0) were cultured in air-liquid interface. Cells were seeded in collagen-coated dishes. At 40% confluence, cell cultures were infected with lentivirus and 48 hours post infection they were selected with 1 ug/ml puromycin. Doxycycline (0.5 ug/ml) was added when cells were confluent (around day 7, D7) to induce the overexpression of DN-GRHL2. Cells were harvested 7 days after doxycycline induction. To construct an inducible Tet-on GFP-labeled dominant-negative mutant of GRHL2, DNA encoding a truncated version of mouse Grhl2 in which amino acids 1-232 were replaced with EGFP was cloned into pTRIPZ inducible lentiviral vector.
Control control EGFP transfected bronchial epithelial cell sample (D7)
Primary human bronchial epithelial (HBE) cells infected with control EGFP lentivirus. HBE cells were obtained from donors without pre-existing lung disease. HBE cells (passage 0) were cultured in air-liquid interface. Cells were seeded in collagen-coated dishes. At 40% confluence, cell cultures were infected with lentivirus and 48 hours post infection they were selected with 1 ug/ml puromycin. Doxycycline (0.5 ug/ml) was added when cells were confluent (around day 7, D7) to induce expression of the EGFP. Seven days after doxycycline induction cells were harvested. To construct a Tet-on GFP labeled control vector, EGFP was cloned into pTRIPZ inducible lentiviral vector.

glioma study 16 (LN-319) / normal astrocyte sample

Relative Expression (log2-ratio):1.9330854
Number of Samples:2 / 3
Experimental glioma study 16 (LN-319)
Human glioma cell line LN319 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

prostate cancer study 8 (p. canc) / prostate cancer study 8 (ptasc)

Relative Expression (log2-ratio):1.8614044
Number of Samples:3 / 2
Experimental prostate cancer study 8 (p. canc)
CD26+ FACS sorted prostate neoplasm cell (p. canc) samples from patients with primary prostate cancer collected after radical prostatectomy.
Control prostate cancer study 8 (ptasc)
CD90+ FACS sorted prostate tumor-associated stromal cell (ptasc) samples from patients with primary prostate cancer collected after radical prostatectomy.

breast cancer study 11 / normal breast tissue

Relative Expression (log2-ratio):-1.8214293
Number of Samples:351 / 4
Experimental breast cancer study 11
Tissue samples from female patients with primary invasive breast cancer after surgery .
Control normal breast tissue
Tissue samples of the breast from healthy female individuals after breast reduction mammoplasty.

Treg activation study 1 (300min) / unstimulated regulatory T-cell sample

Relative Expression (log2-ratio):-1.7798214
Number of Samples:2 / 2
Experimental Treg activation study 1 (300min)
Regulatory T-cells were stimulated for 300min with anti-CD3/anti-CD28/IL-2 (100U/ml). Treg were sorted as CD4+ CD25high cells from peripheral blood of healthy donors.
Control unstimulated regulatory T-cell sample
Unstimulated regulatory T-cell sample derived from sorted CD4+ CD25high cells from peripheral blood of healthy donors.

monocyte activation study 1 (NOD2L/TLR/1L; 24h) / monocyte activation study 1 (NOD2L/TLR/1L; 6h)

Relative Expression (log2-ratio):1.7771654
Number of Samples:5 / 5
Experimental monocyte activation study 1 (NOD2L/TLR/1L; 24h)
Monocytes were isolated from five healthy donors, cultured in RPMI with 10% vitamin D–sufficient (100 nM) human serum and activated for 24 hours with 1 ug/ml muramyl dipeptide (nucleotide-binding oligomerization domain-containing protein 2 ligand, NOD2L) and 1 ug/ml mycobacterial 19-kDa triacylated lipopeptide (TLR2/1 ligand, TLR2/1L). This type of activation is via nucleotide-binding oligomerization domain-containing protein 2 (NOD2) and heterodimeric Toll-like receptor 2 and Toll-like receptor 1 (TLR2/1).
Control monocyte activation study 1 (NOD2L/TLR/1L; 6h)
Monocytes were isolated from five healthy donors, cultured in RPMI with 10% vitamin D–sufficient (100 nM) human serum and activated for 6 hours with 1 ug/ml muramyl dipeptide (nucleotide-binding oligomerization domain-containing protein 2 ligand, NOD2L) and 1 ug/ml mycobacterial 19-kDa triacylated lipopeptide (TLR2/1 ligand, TLR2/1L). This type of activation is via nucleotide-binding oligomerization domain-containing protein 2 (NOD2) and heterodimeric Toll-like receptor 2 and Toll-like receptor 1 (TLR2/1).

clonidine study 5 (1615 uM; hES-Heps) / vehicle (DMSO) treated hES-Heps cell sample

Relative Expression (log2-ratio):-1.7685833
Number of Samples:3 / 8
Experimental clonidine study 5 (1615 uM; hES-Heps)
hES-Heps cells exposed to 1615 uM clonidine (dissolved in 0.5% v/v DMSO) for 72 hours. Chemical was added at day 22 of differentiation. Cells were treated with the IC10 determined by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test (number of replicates, n = 3). ATC code:, , , ,
Control vehicle (DMSO) treated hES-Heps cell sample
hES-Heps cells treated with vehicle (DMSO 0.5% v/v) for 72 hours. Vehicle was added at day 22 of differentiation.