TOP TEN perturbations for Q8N394 (Homo sapiens)

Organism: Homo sapiens
Gene: Q8N394
Selected probe(set): 235775_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of Q8N394 (235775_at) across 5392 perturbations tested by GENEVESTIGATOR:

oxyphilic adenoma study 1 / normal kidney tissue

Relative Expression (log2-ratio):-4.0352774
Number of Samples:4 / 2
Experimental oxyphilic adenoma study 1
Tumor tissue samples from the kidney of patients with oxyphilic adenoma (renal oncocytoma).
Control normal kidney tissue
Normal fetal kidney tissue samples.

glioma study 16 (LN-229) / normal astrocyte sample

Relative Expression (log2-ratio):3.6626024
Number of Samples:2 / 3
Experimental glioma study 16 (LN-229)
Human glioma cell line LN229 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37?C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

TGF-? study 12 / untreated NCI-H358 cell sample

Relative Expression (log2-ratio):-3.3294783
Number of Samples:3 / 3
Experimental TGF-? study 12
NCI-H358 cells were cultured for 2-3 weeks in the presence of 2ng/ml of TGF-?, to induce epithelial-mesenchymal transition (EMT). 24 hours prior analysis cells were re-seeded without TGF-?.
Control untreated NCI-H358 cell sample
NCI-H358 cells grown in standard media.

influenza virus study 10 (A/H5N2) / influenza virus study 4 (A/H1N1)

Relative Expression (log2-ratio):3.299757
Number of Samples:3 / 3
Experimental influenza virus study 10 (A/H5N2)
Human carcinoma cell line A549 infected with influenza A virus subtype influenza virus A/duck/Malaysia/F189/07/2004(H5N2). Samples were taken 10 hours post-infection.
Control influenza virus study 4 (A/H1N1)
Human carcinoma cell line A549 infected with influenza A virus subtype A/WSN/33 (H1N1). Samples were taken 10 hours post-infection.

influenza virus study 11 (A/H5N3) / influenza virus study 4 (A/H1N1)

Relative Expression (log2-ratio):3.2739763
Number of Samples:3 / 3
Experimental influenza virus study 11 (A/H5N3)
Human carcinoma cell line A549 infected with influenza A virus subtype influenza virus A/duck/Malaysia/F119/3/1997(H5N3). Samples were taken 10 hours post-infection.
Control influenza virus study 4 (A/H1N1)
Human carcinoma cell line A549 infected with influenza A virus subtype A/WSN/33 (H1N1). Samples were taken 10 hours post-infection.

ovarian tumor study 28 (endometrioid carcinoma) / ovarian tumor study 28 (adenocarcinoma)

Relative Expression (log2-ratio):3.1421223
Number of Samples:6 / 3
Experimental ovarian tumor study 28 (endometrioid carcinoma)
Primary tumor tissue sample obtained from the ovary of female patients with endometrioid carcinoma.
Control ovarian tumor study 28 (adenocarcinoma)
Primary tumor tissue sample obtained from the ovary of female patients with adenocarcinoma.

angiogenesis study 1 (unspread; VEGF) / angiogenesis study 1 (spread; VEGF)

Relative Expression (log2-ratio):3.122427
Number of Samples:3 / 3
Experimental angiogenesis study 1 (unspread; VEGF)
Partially spread human umbilical vein endothelial cell culture (HUVECs, passage 3) stimulated for 18 hrs with VEGF (50ng/ml) in starvation medium (5% FBS in M199). 1764 um2 was an area of fibronectin for allowing an intermediate cell spread (limited cell adhesion). Prior to treatment cells were cultured in full growth medium (Medium 199= 20 % FBS+ 50 mg/ml ECGS+100 mg/ml heparin+100 units/ml penicillin+100 mg/ml streptomycin) on gelatin-coated surfaces. VEGF is a vascular endothelial growth factor.
Control angiogenesis study 1 (spread; VEGF)
Fully spread human umbilical vein endothelial cell culture (HUVECs, passage 3) stimulated for 18 hrs with VEGF (50ng/ml) in starvation medium (5% FBS in M199). Cells were cultured on fibronectin for 2 hrs (3200 um2 an average area) for allowing a full cell spread (high cell adhesion). Prior to treatment cells were cultured in full growth medium (Medium 199= 20% FBS+ 50 mg/ml ECGS+100 mg/ml heparin+100 units/ml penicillin+100 mg/ml streptomycin) on gelatin-coated surfaces. VEGF is a vascular endothelial growth factor.

influenza virus study 10 (A/H5N2) / influenza virus study 9 (A/pH1N1)

Relative Expression (log2-ratio):3.1110134
Number of Samples:3 / 3
Experimental influenza virus study 10 (A/H5N2)
Human carcinoma cell line A549 infected with influenza A virus subtype influenza virus A/duck/Malaysia/F189/07/2004(H5N2). Samples were taken 10 hours post-infection.
Control influenza virus study 9 (A/pH1N1)
Human carcinoma cell line A549 infected with influenza A virus subtype [A/Singapore/478/2009 (pH1N1)]. Samples were taken 10 hours post-infection.

influenza virus study 11 (A/H5N3) / influenza virus study 9 (A/pH1N1)

Relative Expression (log2-ratio):3.0852327
Number of Samples:3 / 3
Experimental influenza virus study 11 (A/H5N3)
Human carcinoma cell line A549 infected with influenza A virus subtype influenza virus A/duck/Malaysia/F119/3/1997(H5N3). Samples were taken 10 hours post-infection.
Control influenza virus study 9 (A/pH1N1)
Human carcinoma cell line A549 infected with influenza A virus subtype [A/Singapore/478/2009 (pH1N1)]. Samples were taken 10 hours post-infection.

rheumatoid arthritis study 31 / TNF-? study 20

Relative Expression (log2-ratio):3.0731678
Number of Samples:4 / 3
Experimental rheumatoid arthritis study 31
Monocyte samples isolated from patients with rheumatoid arthritis (RA). Monocyte from whole blood were depleted from granulocytes by CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling. Patients fulfilled the revised American College of Rheumatology criteria (ACR) for RA.
Control TNF-? study 20
Monocyte samples isolated from healthy donors and stimulated in vitro by 100 ng/ml TNF? in whole blood for 1.5 hour. Following stimulation, monocyte were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.