TOP TEN perturbations for Q96LB0 (Homo sapiens)

Organism: Homo sapiens
Gene: Q96LB0
Selected probe(set): 1553293_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of Q96LB0 (1553293_at) across 5610 perturbations tested by GENEVESTIGATOR:

PMA study 6 (500ng/ml) / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):5.1488633
Number of Samples:3 / 17
Experimental PMA study 6 (500ng/ml)
Bronchial epithelial cells (NHBE) treated with tetradecanoylphorbol acetate (500ng/ml; vendor: Sigma / catalog number: P1585 / catalog name: Phorbol 12-myristate 13-acetate [16561-29-8]) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial). ATC code:---
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).

polyinosinic-polycytidylic acid study 1 (10ug/ml) / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):4.8326945
Number of Samples:3 / 17
Experimental polyinosinic-polycytidylic acid study 1 (10ug/ml)
Bronchial epithelial cells (NHBE) treated with polyinosinic-polycytidylic acid (Poly(I:C); 10ug/ml; vendor: InvivoGen / catalog number: tlrl-pic / catalog name: Poly(I:C) High Molecular Weight [31852-29-6]) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial). ATC code:
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).

TNF-α;TGF-β study 1 (3D) / untreated A549 cell sample (3D)

Relative Expression (log2-ratio):4.226783
Number of Samples:2 / 2
Experimental TNF-α;TGF-β study 1 (3D)
A549 cells were grown as 3D-culture/spheroids to 80% confluency and incubated with 10 ng/mL TNF and 2 ng/mL TGF two times each for 48 hours. Samples were taken 4 days after the treatment.
Control untreated A549 cell sample (3D)
A549 cells were grown as 3D-culture to 80% confluency and kept in culture for 4 days.

engineered skin substitute study 2 (late) / engineered skin substitute study 1 (late)

Relative Expression (log2-ratio):-3.862811
Number of Samples:5 / 3
Experimental engineered skin substitute study 2 (late)
Human skin substitutes tissue samples collected after 42 and 56 days after transplantation to athymic mice.
Control engineered skin substitute study 1 (late)
Human skin substitutes tissue samples collected after 14 days in culture.

Li-Fraumeni syndrome study 1 / normal breast epithelial cell sample

Relative Expression (log2-ratio):-3.817913
Number of Samples:7 / 4
Experimental Li-Fraumeni syndrome study 1
Morphologically normal breast epithelial cells were isolated from non-involved tissue of Li-Fraumeni syndrome patients with breast cancer. Li-Fraumeni syndrome is cancer predisposing syndrome caused by germline mutation of the p53. Patient (50): 31-years old female with breast cancer and p53 mutation at codon 133 in exon 5, in one of the two alleles (Met133Thr). The donor had also family history of breast cancer and p53 mutations through at least last three generations. Patient (IUSM): 29-years old Caucasian female with non-invasive ductal carcinoma and bilateral Paget disease of the nipples; with heterozygous p53 12141delG germline frameshift mutation. The donor had also a maternal aunt with bilateral breast cancer in her 30's and a male sibling with osteogenic sarcoma of a leg at age 13, who later died of a brain tumor at age 19.
Control normal breast epithelial cell sample
Normal breast epithelial cells were isolated from donor with no history of cancer and wild-type p53.

TNF-ɑ study 1 / untreated dermal lymphatic endothelial cell sample

Relative Expression (log2-ratio):3.743455
Number of Samples:3 / 3
Experimental TNF-ɑ study 1
Normal dermal lymphatic endothelial cells stimulated with TNF-ɑ, 1 ng/ml for 48h.
Control untreated dermal lymphatic endothelial cell sample
Normal dermal lymphatic endothelial cells cultured in media alone for 48h.

engineered skin substitute study 2 (intermediate) / engineered skin substitute study 1 (intermediate)

Relative Expression (log2-ratio):-3.6615896
Number of Samples:6 / 3
Experimental engineered skin substitute study 2 (intermediate)
Human skin substitutes tissue samples collected after 14 and 28 days after transplantation to athymic mice.
Control engineered skin substitute study 1 (intermediate)
Human skin substitutes tissue samples collected after 7 days in culture.

influenza virus study 9 (A/pH1N1) / influenza virus study 4 (A/H1N1)

Relative Expression (log2-ratio):-3.0411758
Number of Samples:3 / 3
Experimental influenza virus study 9 (A/pH1N1)
Human carcinoma cell line A549 infected with influenza A virus subtype [A/Singapore/478/2009 (pH1N1)]. Samples were taken 10 hours post-infection.
Control influenza virus study 4 (A/H1N1)
Human carcinoma cell line A549 infected with influenza A virus subtype A/WSN/33 (H1N1). Samples were taken 10 hours post-infection.

influenza virus study 10 (A/H5N2) / influenza virus study 4 (A/H1N1)

Relative Expression (log2-ratio):-2.912931
Number of Samples:3 / 3
Experimental influenza virus study 10 (A/H5N2)
Human carcinoma cell line A549 infected with influenza A virus subtype influenza virus A/duck/Malaysia/F189/07/2004(H5N2). Samples were taken 10 hours post-infection.
Control influenza virus study 4 (A/H1N1)
Human carcinoma cell line A549 infected with influenza A virus subtype A/WSN/33 (H1N1). Samples were taken 10 hours post-infection.

influenza virus study 9 (A/H9N2) / influenza virus study 4 (A/H1N1)

Relative Expression (log2-ratio):-2.9115043
Number of Samples:3 / 3
Experimental influenza virus study 9 (A/H9N2)
Human carcinoma cell line A549 infected with influenza A virus subtype A/Duck/Malaysia/01 (H9N2). Samples were taken 10 hours post-infection.
Control influenza virus study 4 (A/H1N1)
Human carcinoma cell line A549 infected with influenza A virus subtype A/WSN/33 (H1N1). Samples were taken 10 hours post-infection.