TOP TEN perturbations for Q9NNW7 (Homo sapiens)

Organism: Homo sapiens
Gene: Q9NNW7
Selected probe(set): 211177_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of Q9NNW7 (211177_s_at) across 5610 perturbations tested by GENEVESTIGATOR:

CD44s overexpr. study 1 / normal HEK-293 cell sample

Relative Expression (log2-ratio):2.8334017
Number of Samples:3 / 3
Experimental CD44s overexpr. study 1
Human embryonic kidney cell line HEK-293 transfected with pcDNA3.1(-) vector containing codon-optimized human CD44 sequence for expression.
Control normal HEK-293 cell sample
Untransfected, native human embryonic kidney cell line HEK-293 cell samples.

influenza virus study 10 (A/H5N2) / influenza virus study 4 (A/H1N1)

Relative Expression (log2-ratio):-1.786973
Number of Samples:3 / 3
Experimental influenza virus study 10 (A/H5N2)
Human carcinoma cell line A549 infected with influenza A virus subtype influenza virus A/duck/Malaysia/F189/07/2004(H5N2). Samples were taken 10 hours post-infection.
Control influenza virus study 4 (A/H1N1)
Human carcinoma cell line A549 infected with influenza A virus subtype A/WSN/33 (H1N1). Samples were taken 10 hours post-infection.

angiopoietin-1 study 1 (sparse) / angiopoietin-1 study 1 (confl.)

Relative Expression (log2-ratio):1.7436647
Number of Samples:2 / 2
Experimental angiopoietin-1 study 1 (sparse)
Sparse human umbilical vein endothelial cells stimulated with 100 ng /ml COMP-Angiopoietin-1 for 1h after starvation overnight.
Control angiopoietin-1 study 1 (confl.)
Confluent human umbilical vein endothelial cells stimulated with 100 ng /ml COMP-Angiopoietin-1 for 1h after starvation overnight.

glioma study 16 (LN-229) / normal astrocyte sample

Relative Expression (log2-ratio):1.6318436
Number of Samples:2 / 3
Experimental glioma study 16 (LN-229)
Human glioma cell line LN229 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37?C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

kidney transplantation study 15 (8 week) / normal monocyte (CD14+) sample

Relative Expression (log2-ratio):1.6110115
Number of Samples:2 / 5
Experimental kidney transplantation study 15 (8 week)
CD14+ monocyte samples derived from kidney transplant patients 8 weeks post-transplantation. Samples were collected 8 week after transplantation and administration of immunosuppressive therapy (day 1-4: methylprednisolone (60 mg); 3 doses: rabbit polyclonal anti-thymocyte globulin (ThymoglobulinH; 6 mg/kg); mycophenolate mofetil (CellCeptH); and tacrolimus (PrografH).
Control normal monocyte (CD14+) sample
CD14+ monocyte samples derived from healthy control subjects.

angiogenesis study 1 (unspread; VEGF) / angiogenesis study 1 (spread; VEGF)

Relative Expression (log2-ratio):-1.5756721
Number of Samples:3 / 3
Experimental angiogenesis study 1 (unspread; VEGF)
Partially spread human umbilical vein endothelial cell culture (HUVECs, passage 3) stimulated for 18 hrs with VEGF (50ng/ml) in starvation medium (5% FBS in M199). 1764 um2 was an area of fibronectin for allowing an intermediate cell spread (limited cell adhesion). Prior to treatment cells were cultured in full growth medium (Medium 199= 20 % FBS+ 50 mg/ml ECGS+100 mg/ml heparin+100 units/ml penicillin+100 mg/ml streptomycin) on gelatin-coated surfaces. VEGF is a vascular endothelial growth factor.
Control angiogenesis study 1 (spread; VEGF)
Fully spread human umbilical vein endothelial cell culture (HUVECs, passage 3) stimulated for 18 hrs with VEGF (50ng/ml) in starvation medium (5% FBS in M199). Cells were cultured on fibronectin for 2 hrs (3200 um2 an average area) for allowing a full cell spread (high cell adhesion). Prior to treatment cells were cultured in full growth medium (Medium 199= 20% FBS+ 50 mg/ml ECGS+100 mg/ml heparin+100 units/ml penicillin+100 mg/ml streptomycin) on gelatin-coated surfaces. VEGF is a vascular endothelial growth factor.

LPS study 4 (shRNA contr.) / mock treated / transduced MONO-MAC-6 cell sample

Relative Expression (log2-ratio):-1.5354939
Number of Samples:2 / 2
Experimental LPS study 4 (shRNA contr.)
MONO-MAC-6 (MM6) cells were transduced with a control shRNA and then treated with 10 ng/ml lipopolysaccharide (LPS). ATC code:---
Control mock treated / transduced MONO-MAC-6 cell sample
MONO-MAC-6 (MM6) cells were transduced with a control shRNA and then mock treated.

FoxO3 overexpr. study 1 / 4-hydroxytamoxifen study 1 (empty vector)

Relative Expression (log2-ratio):-1.4698973
Number of Samples:3 / 3
Experimental FoxO3 overexpr. study 1
Human umbilical vein endothelial cells (HUVECs) transfected with retroviral pBP-FoxO3.A3.ER vector for 4OHT (4-hydroxy-(Z)-tamoxifen) inducible expression of FoxO3 (forkhead box O3), treated with 4OHT for 12 hours. 72 hours postinfection, cells were selected for puromycin resistance by adding 2 g/ml puromycin overnight. Further, cells were reseeded in puromycin-free medium, treated for 12 hours with 4OHT, and harvested for total RNA isolation. Cells from passage 3 were used for infection and maximally passaged once for the experiments. ATC code:---
Control 4-hydroxytamoxifen study 1 (empty vector)
Human umbilical vein endothelial cells (HUVECs) transfected with empty retroviral pBabe puro vector designed for 4OHT (4-hydroxy-(Z)-tamoxifen) inducible expression, treated with 4OHT for 12 hours. 72 hours postinfection, cells were selected for puromycin resistance by adding 2 g/ml puromycin overnight. Further, cells were reseeded in puromycin-free medium, treated for 12 hours with 4OHT, and harvested for total RNA isolation. Cells from passage 3 were used for infection and maximally passaged once for the experiments. ATC code:---

influenza virus study 11 (A/H5N3) / influenza virus study 4 (A/H1N1)

Relative Expression (log2-ratio):-1.4567451
Number of Samples:3 / 3
Experimental influenza virus study 11 (A/H5N3)
Human carcinoma cell line A549 infected with influenza A virus subtype influenza virus A/duck/Malaysia/F119/3/1997(H5N3). Samples were taken 10 hours post-infection.
Control influenza virus study 4 (A/H1N1)
Human carcinoma cell line A549 infected with influenza A virus subtype A/WSN/33 (H1N1). Samples were taken 10 hours post-infection.

cicloheximide study 1 / vehicle (DMSO) treated MCF-7 cell sample

Relative Expression (log2-ratio):1.408679
Number of Samples:4 / 4
Experimental cicloheximide study 1
MCF7 breast cancer cells were treated for 1h with cicloheximide (10 ug/ml) before vehicle treatment (0.1% ethanol) for 24h. ATC code:---
Control vehicle (DMSO) treated MCF-7 cell sample
MCF7 breast cancer cells treated with vehicle DMSO (0.1%) for 1h and subsequently with vehicle (0.1% ethanol) for 24h.