TOP TEN perturbations for Q9NQS5 (Homo sapiens)

Organism: Homo sapiens
Gene: Q9NQS5
Selected probe(set): 223767_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of Q9NQS5 (223767_at) across 5880 perturbations tested by GENEVESTIGATOR:

Langerhans cell histiocytosis study 1 / normal plasmocytoid dendritic cell sample

Relative Expression (log2-ratio):7.3898373
Number of Samples:7 / 3
Experimental Langerhans cell histiocytosis study 1
Langerhans cell histiocytes (LCH) were isolated from LCH lesions of patients undergoing surgery. All patients had single system disease, five patients had bone lesion, one had skin lesion and one had mucosal manifestation. Langerhans cells histiocytes were purified by FACS as CD1a+ / CD270 + population with > 95% purity.
Control normal plasmocytoid dendritic cell sample
Normal plasmocytoid dendritic cells were isolated from peripheral blood of healthy adult donors. Plasmocytoid dendritic cells were defined as HLA-DR+ / CD45RAhi / CD11c- / BDCA2+ population.

Langerhans cell histiocytosis study 1 / normal myeloid dendritic cell sample

Relative Expression (log2-ratio):6.2989426
Number of Samples:7 / 3
Experimental Langerhans cell histiocytosis study 1
Langerhans cell histiocytes (LCH) were isolated from LCH lesions of patients undergoing surgery. All patients had single system disease, five patients had bone lesion, one had skin lesion and one had mucosal manifestation. Langerhans cells histiocytes were purified by FACS as CD1a+ / CD270 + population with > 95% purity.
Control normal myeloid dendritic cell sample
Normal myeloid dendritic cells were isolated from peripheral blood of healthy adult donors. Myeloid dendritic cells were defined as HLA-DR+ / CD11c + / BDCA1+ / BDCA3- population.

smoking study 74 (smoker; stim. blood culture) / smoking study 74 (smoker; unstim. blood culture)

Relative Expression (log2-ratio):6.2287207
Number of Samples:20 / 18
Experimental smoking study 74 (smoker; stim. blood culture)
Stimulated peripheral blood cultures from current or former smokers without COPD. One milliliter of peripheral blood was collected into anti-CD3 and anti-CD28 TruCulture tubes and incubated for 24 hours at 37ºC. The TruCulture is an ex-vivo culture system which should preserve physiological cellular responses of immune cells to stress or stimulation at the time and place of sample collection and minimize the bias and variability caused by sample manipulation. Available with/without specific stimulant.
Control smoking study 74 (smoker; unstim. blood culture)
Unstimulated peripheral blood cultures from current or former smokers without COPD. One milliliter of peripheral blood was collected into null TruCulture tubes and incubated for 24 hours at 37ºC. The TruCulture is an ex-vivo culture system which should preserve physiological cellular responses of immune cells to stress or stimulation at the time and place of sample collection and to minimize the bias and variability caused by sample manipulation. Available with/without specific stimulant.

chronic obstructive pulmonary disease study 21 (smoker; stim. blood culture) / chronic obstructive pulmonary disease study 21 (smoker; unstim. blood culture)

Relative Expression (log2-ratio):6.099695
Number of Samples:42 / 46
Experimental chronic obstructive pulmonary disease study 21 (smoker; stim. blood culture)
Stimulated peripheral blood cultures from patients with COPD. Persons included in the study were former or current smokers with smoking status > 10 pack-yr and with FEV1/FVC < 70 post bronchodilator. One milliliter of peripheral blood was collected into anti-CD3 and anti-CD28 TruCulture tubes and incubated for 24 hours at 37ºC. The TruCulture is an ex-vivo culture system which should preserve physiological cellular responses of immune cells to stress or stimulation at the time and place of sample collection and minimize the bias and variability caused by sample manipulation. Available with/without specific stimulant.
Control chronic obstructive pulmonary disease study 21 (smoker; unstim. blood culture)
Unstimulated peripheral blood cultures from patients with COPD. Persons included in the study were former or current smokers with smoking status > 10 pack-yr and with FEV1/FVC < 70 post bronchodilator. One milliliter of peripheral blood was collected into null TruCulture tubes and incubated for 24 hours at 37ºC. The TruCulture is an ex-vivo culture system which should preserve physiological cellular responses of immune cells to stress or stimulation at the time and place of sample collection and minimize the bias and variability caused by sample manipulation. Available with/without specific stimulant.

rheumatoid arthritis study 31 / TNF-ɑ study 20

Relative Expression (log2-ratio):-5.4184523
Number of Samples:4 / 3
Experimental rheumatoid arthritis study 31
Monocyte samples isolated from patients with rheumatoid arthritis (RA). Monocyte from whole blood were depleted from granulocytes by CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling. Patients fulfilled the revised American College of Rheumatology criteria (ACR) for RA.
Control TNF-ɑ study 20
Monocyte samples isolated from healthy donors and stimulated in vitro by 100 ng/ml TNFα in whole blood for 1.5 hour. Following stimulation, monocyte were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.

pediatric meningococcal sepsis study 2 (early) / normal blood sample

Relative Expression (log2-ratio):5.344742
Number of Samples:2 / 3
Experimental pediatric meningococcal sepsis study 2 (early)
Whole blood drawn from children with meningococcal sepsis at time of admission to the pediatric intensive care unit (0h).
Control normal blood sample
Whole blood drawn from matched control subjects.

TNF-ɑ study 20 / normal monocyte sample

Relative Expression (log2-ratio):5.279971
Number of Samples:3 / 7
Experimental TNF-ɑ study 20
Monocyte samples isolated from healthy donors and stimulated in vitro by 100 ng/ml TNFα in whole blood for 1.5 hour. Following stimulation, monocyte were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.
Control normal monocyte sample
Monocyte samples isolated from healthy subjects. Peripheral blood monocytes were isolated immediately after drawing blood by depletion from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.

LPS study 4 (shRNA contr.) / mock treated / transduced MONO-MAC-6 cell sample

Relative Expression (log2-ratio):5.08959
Number of Samples:2 / 2
Experimental LPS study 4 (shRNA contr.)
MONO-MAC-6 (MM6) cells were transduced with a control shRNA and then treated with 10 ng/ml lipopolysaccharide (LPS). ATC code:---
Control mock treated / transduced MONO-MAC-6 cell sample
MONO-MAC-6 (MM6) cells were transduced with a control shRNA and then mock treated.

G-CSF study 1 / untreated leukocyte sample

Relative Expression (log2-ratio):4.9360523
Number of Samples:5 / 5
Experimental G-CSF study 1
Peripheral blood samples from normal donors for allogenic PBSC transplantation obtained after administration of granulocyte-colony stimulating factor (G-CSF; 10μg/kg/day for five days). Whole blood leukocyte RNA was purified from each sample and used to generate cRNAs.
Control untreated leukocyte sample
Peripheral blood samples from normal donors for allogenic PBSC transplantation obtained before administration of granulocyte-colony stimulating factor (G-CSF). Whole blood leukocyte RNA was purified from each sample and used to generate cRNAs.

septic shock study 2 (0h) / normal blood sample

Relative Expression (log2-ratio):4.876359
Number of Samples:14 / 25
Experimental septic shock study 2 (0h)
Blood samples from intensive care unit patients shortly after severe septic shock (SAPS II-high). Severity was assessed using SAPS II score. Septic shock was defined as the combination of SIRS and an infection. To be diagnosed with SIRS patients had to show at least two of the following clinical situation: hypothermia (<36°C) or hyperthermia (>38°C), tachycardia (>90/min), tachypnea (>20 breaths/min) and/or arterial PCO2 of 32 mmHg or lower and/or mechanical ventilation, and leukocytosis (>12,000/mm3) or leukopenia (<4,000/mm3). Septic shock was defined as acute circulatory failure (systolic blood pressure <90 mmHg, mean arterial pressure <65 mmHg, or a reduction in systolic blood pressure >40 mmHg from baseline). In order to avoid confounding effects, patients with human immunodeficiency syndrome, hematologic malignancies evolving from insulin-dependent diabetes, dialyzed chronic renal failure, chronic liver disease stages III and more or patients receiving immunosuppressive therapy were excluded from the study. Other clinical characteristics of the group: non-survivor (28.5%), charlson median (1.3-3.8 %), SAPS II on admission median (49-63), duration length in ICU median (6-30), SOFA H6 (9-13), admission (surgery: 57.1%; medical: 42.9%); type of infection (community acquired: 64.29%; hospital acquired: 35.71%), suspected infection (Bacilli Gram (-): 64%; Cocci Gram (+):64%; Fungi: 0%), cell counts (white blood cells: 3.23-15.68 giga/L; lymphocytes: 0.4-1.33; polymorphonuclear cells: 2.5-12.61; monocytes: 0.19-0.66).
Control normal blood sample
Blood samples from healthy volunteers.