TOP TEN perturbations for Q9UL17 (Homo sapiens)

Organism: Homo sapiens
Gene: Q9UL17
Selected probe(set): 220684_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of Q9UL17 (220684_at) across 5654 perturbations tested by GENEVESTIGATOR:

T-cell activation study 3 / resting CD4 T-lymphocyte (crude fraction) sample

Relative Expression (log2-ratio):3.0496168
Number of Samples:2 / 2
Experimental T-cell activation study 3
CD4+ T-cell samples prepared from crude lymphocyte fraction. Cells were activated with anti-CD3/28 beads for 18hrs.
Control resting CD4 T-lymphocyte (crude fraction) sample
Resting CD4 T-lymphocytes prepared from crude lymphocyte fraction.

cutaneous sarcoidosis study 1 (lesional) / normal skin tissue

Relative Expression (log2-ratio):2.613512
Number of Samples:13 / 5
Experimental cutaneous sarcoidosis study 1 (lesional)
Lesional skin biopsies from patients with acute cutaneous sarcoidosis.
Control normal skin tissue
Skin biopsy samples from healthy control subjects.

T-cell activation study 4 / normal resting T-cell sample

Relative Expression (log2-ratio):2.5728245
Number of Samples:2 / 2
Experimental T-cell activation study 4
T-cell samples antiCD3 activated for 30hrs.
Control normal resting T-cell sample
T cells resting for 30h.

IFN-g study 11 / normal monocyte sample

Relative Expression (log2-ratio):2.5169563
Number of Samples:7 / 7
Experimental IFN-g study 11
Monocyte samples isolated from donors and stimulated in vitro by 100 ng/ml IFNγ in whole blood for 1.5 hour. Following stimulation, monocyte were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.
Control normal monocyte sample
Monocyte samples isolated from healthy subjects. Peripheral blood monocytes were isolated immediately after drawing blood by depletion from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.

rheumatoid arthritis study 31 / IFN-g study 11

Relative Expression (log2-ratio):-2.4506187
Number of Samples:4 / 7
Experimental rheumatoid arthritis study 31
Monocyte samples isolated from patients with rheumatoid arthritis (RA). Monocyte from whole blood were depleted from granulocytes by CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling. Patients fulfilled the revised American College of Rheumatology criteria (ACR) for RA.
Control IFN-g study 11
Monocyte samples isolated from donors and stimulated in vitro by 100 ng/ml IFNγ in whole blood for 1.5 hour. Following stimulation, monocyte were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.

IFN-g study 11 / unstimulated monocyte sample

Relative Expression (log2-ratio):2.4294863
Number of Samples:7 / 11
Experimental IFN-g study 11
Monocyte samples isolated from donors and stimulated in vitro by 100 ng/ml IFNγ in whole blood for 1.5 hour. Following stimulation, monocyte were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.
Control unstimulated monocyte sample
Monocyte samples isolated from healthy donors and incubated in whole blood for 1.5 hour without any stimulation. Following incubation, monocyte were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.

kidney transplantation study 11 (1 week) / normal blood sample

Relative Expression (log2-ratio):-2.384142
Number of Samples:11 / 5
Experimental kidney transplantation study 11 (1 week)
Whole blood samples derived from kidney transplant patients 1 week post-transplantation. Samples were collected 1 week after transplantation and administration of immunosuppressive therapy (day 1-4: methylprednisolone (60 mg); 3 doses: rabbit polyclonal anti-thymocyte globulin (ThymoglobulinH; 6 mg/kg); mycophenolate mofetil (CellCeptH); and tacrolimus (PrografH).
Control normal blood sample
Blood samples derived from healthy control subjects.

cutaneous sarcoidosis study 1 (lesional) / cutaneous sarcoidosis study 1 (non-lesional)

Relative Expression (log2-ratio):2.3471537
Number of Samples:13 / 9
Experimental cutaneous sarcoidosis study 1 (lesional)
Lesional skin biopsies from patients with acute cutaneous sarcoidosis.
Control cutaneous sarcoidosis study 1 (non-lesional)
Non-lesional skin biopsies from patients with acute cutaneous sarcoidosis.

acute coronary syndrome study 1 (STEMI) / peripheral blood leukocyte sample (STEMI)

Relative Expression (log2-ratio):-2.1996098
Number of Samples:4 / 4
Experimental acute coronary syndrome study 1 (STEMI)
Thrombus-derived leukocytes obtained from patients with ST-segment elevation myocardial infarction (STEMI) undergoing primary percutaneous coronary intervention (PCI). Leukocytes were derived from an occluding coronary thrombus, immediately after diagnostic angiography and identification of the culprit lesion, the presence of total coronary occlusion and angiographic signs of an occluding thrombus. The culprit coronary artery was intubated with a 6 F guiding catheter and wired with a 0.014 inch guide wire and prior to any subsequent interventional procedures, thrombi were removed from the site of coronary occlusion with an aspiration catheter and immersed in phosphate-buffered saline immediately.
Control peripheral blood leukocyte sample (STEMI)
Peripheral blood leukocytes derived from patients with ST-elevation myocardial infarction (STEMI).

E. coli study 1 / unstimulated, normal monocyte-derived macrophage sample

Relative Expression (log2-ratio):2.156783
Number of Samples:12 / 16
Experimental E. coli study 1
Monocyte-derived macrophage samples derived from healthy control subjects cultured for 4 hours with 2.5 × 105 heat-killed E. coli.
Control unstimulated, normal monocyte-derived macrophage sample
Monocyte-derived macrophage samples derived from healthy control subjects cultured for 4 hours unstimulated.