TOP TEN perturbations for Q9ULX9 (Homo sapiens)

Organism: Homo sapiens
Gene: Q9ULX9
Selected probe(set): 36711_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of Q9ULX9 (36711_at) across 5610 perturbations tested by GENEVESTIGATOR:

IFN-g study 11 / normal monocyte sample

Relative Expression (log2-ratio):8.116105
Number of Samples:7 / 7
Experimental IFN-g study 11
Monocyte samples isolated from donors and stimulated in vitro by 100 ng/ml IFNγ in whole blood for 1.5 hour. Following stimulation, monocyte were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.
Control normal monocyte sample
Monocyte samples isolated from healthy subjects. Peripheral blood monocytes were isolated immediately after drawing blood by depletion from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.

IFNa-2a study 1 / normal monocyte sample

Relative Expression (log2-ratio):7.452591
Number of Samples:7 / 7
Experimental IFNa-2a study 1
Monocyte samples isolated from healthy donors and stimulated in vitro by 100 ng/ml IFNα2a in whole blood for 1.5 hour. Following stimulation, monocyte were depleted from granulocytes using CD15 MACS beads, and they were sorted from remaining peripheral blood leukocyte by CD14 labeling.
Control normal monocyte sample
Monocyte samples isolated from healthy subjects. Peripheral blood monocytes were isolated immediately after drawing blood by depletion from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.

EBNA2 overexpr. study 1 (4h) / control virus transfected EREB2-5 cell sample (24h)

Relative Expression (log2-ratio):7.180612
Number of Samples:3 / 3
Experimental EBNA2 overexpr. study 1 (4h)
EREB2-5 cell line stably transfected with the chimeric Epstein-Barr virus-encoded nuclear antigen 2 (EBNA2) and control expression plasmid coding for bacterial chloramphenicol acetyltransferase (CAT) gene and . CAT was used as a negative control. Briefly, before the CAT and EBNA2 induction, cells were maintained in RPMI media and deprived of estrogen for 3 days before doxycycline and estrogen was added. Expression of EBNA2 was induced after addition of estrogen and expression of CAT was induced by the addition of doxycycline to a final concentration of 100 ng/mL medium. Doxycycline induced cells were harvested 4 hours after induction. Because EBNA2 is essential for the proliferation of EBV-infected cells, EREB2-5 cells grow only in the presence of estrogen. Before the preparation of RNA, NGFR cells were purified by MACS separation to enrich the cells with a transcriptional response to doxycycline.
Control control virus transfected EREB2-5 cell sample (24h)
EREB2-5 cell line stably transfected with control expression plasmid coding for bacterial chloramphenicol acetyltransferase (CAT) gene. CAT was used as a negative control. Briefly, before the CAT induction, cells were maintained in RPMI media and deprived of estrogen for 3 days before doxycycline was added. Expression of CAT was induced by the addition of doxycycline to a final concentration of 100 ng/mL medium. Doxycycline induced cells were harvested 24 hours after induction. Estrogen withdrawal leads to the inactivation of EBNA2, resulting in cell cycle arrest. Before the preparation of RNA, NGFR cells were purified by MACS separation to enrich the cells with a transcriptional response to doxycycline.

exercise study 31 (post-exercise; 24h; male) / exercise study 31 (post-exercise; 4h; male)

Relative Expression (log2-ratio):-6.5503397
Number of Samples:3 / 3
Experimental exercise study 31 (post-exercise; 24h; male)
Biceps brachii muscle biopsy was taken from young healthy men who performed an acute bout of resistance exercise (RE) with previously trained arm at 24h post-exercise. Men underwent a 12-week (2 training sessions/week) supervised progressive unilateral arm resistance exercise (RE) training program (FAMuSS study). One week after the last session of training, subjects performed the acute bout of unilateral arm RE in which the trained arm exercised at the same relative intensity. The acute RE protocol was identical to the final training session of the FAMuSS program. Briefly, during the acute unilateral arm RE session, subjects performed five upper arm exercises including the biceps preacher curl, biceps concentration curl, standing biceps curl, overhead triceps extension, and triceps kickback. Each exercise was performed for three sets of six repetitions using the six repetition maximum (RM) weight. Before the first biceps and the first triceps exercise, subjects completed two warmup sets and then rested for 3 min. Subjects rested for 2 min between sets. A muscle biopsy was obtained 24h post exercise from the biceps brachii of the trained/exercised arm. All biopsy samples were immediately weighed and snap frozen in liquid nitrogencooled isopentane and stored at −80°C for subsequent analyses. Subjects met the following inclusion criteria: 1) between the ages of 18 and 40 yr, 2) no chronic diseases, 3) no prior resistance training history, and 4) no medications or dietary supplements which may affect musculature.
Control exercise study 31 (post-exercise; 4h; male)
Biceps brachii muscle biopsy was taken from young healthy men who performed an acute bout of resistance exercise (RE) with previously trained arm at 4h post-exercise. Men underwent a 12-week (2 training sessions/week) supervised progressive unilateral arm resistance exercise (RE) training program (FAMuSS study). One week after the last session of training, subjects performed the acute bout of unilateral arm RE in which the trained arm exercised at the same relative intensity. The acute RE protocol was identical to the final training session of the FAMuSS program. Briefly, during the acute unilateral arm RE session, subjects performed five upper arm exercises including the biceps preacher curl, biceps concentration curl, standing biceps curl, overhead triceps extension, and triceps kickback. Each exercise was performed for three sets of six repetitions using the six repetition maximum (RM) weight. Before the first biceps and the first triceps exercise, subjects completed two warmup sets and then rested for 3 min. Subjects rested for 2 min between sets. A muscle biopsy was obtained 4h post exercise from the biceps brachii of the trained/exercised arm. All biopsy samples were immediately weighed and snap frozen in liquid nitrogencooled isopentane and stored at −80°C for subsequent analyses. Subjects met the following inclusion criteria: 1) between the ages of 18 and 40 yr, 2) no chronic diseases, 3) no prior resistance training history, and 4) no medications or dietary supplements which may affect musculature.

LPS study 4 (shRNA contr.) / mock treated / transduced MONO-MAC-6 cell sample

Relative Expression (log2-ratio):6.1514645
Number of Samples:2 / 2
Experimental LPS study 4 (shRNA contr.)
MONO-MAC-6 (MM6) cells were transduced with a control shRNA and then treated with 10 ng/ml lipopolysaccharide (LPS). ATC code:---
Control mock treated / transduced MONO-MAC-6 cell sample
MONO-MAC-6 (MM6) cells were transduced with a control shRNA and then mock treated.

TNF-ɑ study 20 / normal monocyte sample

Relative Expression (log2-ratio):6.1436777
Number of Samples:3 / 7
Experimental TNF-ɑ study 20
Monocyte samples isolated from healthy donors and stimulated in vitro by 100 ng/ml TNFα in whole blood for 1.5 hour. Following stimulation, monocyte were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.
Control normal monocyte sample
Monocyte samples isolated from healthy subjects. Peripheral blood monocytes were isolated immediately after drawing blood by depletion from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.

LPS study 4 / mock treated MONO-MAC-6 cell sample

Relative Expression (log2-ratio):6.0867834
Number of Samples:2 / 2
Experimental LPS study 4
MONO-MAC-6 (MM6) cells were treated with 10 ng/ml lipopolysaccharide (LPS). ATC code:---
Control mock treated MONO-MAC-6 cell sample
MONO-MAC-6 (MM6) cells mock treated.

CD44s overexpr. study 1 / normal HEK-293 cell sample

Relative Expression (log2-ratio):5.8828363
Number of Samples:3 / 3
Experimental CD44s overexpr. study 1
Human embryonic kidney cell line HEK-293 transfected with pcDNA3.1(-) vector containing codon-optimized human CD44 sequence for expression.
Control normal HEK-293 cell sample
Untransfected, native human embryonic kidney cell line HEK-293 cell samples.

echinomycin study 1 / deferoxamine study 5

Relative Expression (log2-ratio):-5.8000507
Number of Samples:3 / 3
Experimental echinomycin study 1
Echinomycin (100nM; 2h) treated human astroglioma (U251) cells, stimulated with deferoxamine (DFO; 300mM; 16h). ATC code:---
Control deferoxamine study 5
Untreated human astroglioma (U251) cells, stimulated with deferoxamine (DFO; 300mM; 16h). ATC code:

rheumatoid arthritis study 31 / IFN-g study 11

Relative Expression (log2-ratio):-5.663933
Number of Samples:4 / 7
Experimental rheumatoid arthritis study 31
Monocyte samples isolated from patients with rheumatoid arthritis (RA). Monocyte from whole blood were depleted from granulocytes by CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling. Patients fulfilled the revised American College of Rheumatology criteria (ACR) for RA.
Control IFN-g study 11
Monocyte samples isolated from donors and stimulated in vitro by 100 ng/ml IFNγ in whole blood for 1.5 hour. Following stimulation, monocyte were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.