TOP TEN perturbations for Pias3 (Mus musculus)

Organism: Mus musculus
Gene: Pias3
Selected probe(set): 1451115_at
Platform: Affymetrix Mouse Genome 430 2.0 Array

Expression of Pias3 (1451115_at) across 2604 perturbations tested by GENEVESTIGATOR:

IL-12; IL-18 study 1 / uninfected, IL-2 grown splenic natural killer cell sample (C57BL/6)

Relative Expression (log2-ratio):-2.4055958
Number of Samples:3 / 3
Experimental IL-12; IL-18 study 1
Splenic natural killer cells grown on IL-2 in vitro were treated will IL-12 and IL-18.
Control uninfected, IL-2 grown splenic natural killer cell sample (C57BL/6)
Splenic natural killer cells grown on IL-2 in vitro.

retina development study 2 (Nrl-EGFP; P9) / retina development study 2 (Nrl-EGFP; P6)

Relative Expression (log2-ratio):-2.3678427
Number of Samples:4 / 4
Experimental retina development study 2 (Nrl-EGFP; P9)
Retinal rod cells isolated at postnatal day 9 from Nrl-EGFP mouse strain. Mice were sacrificed by decapitation 2–3 hours subsequent to light onset. Retinas from 4–5 independent biological replicates were immediately dissected from enucleated eyes, and dissociated with trypsin. Reactions were quenched with SBTI (1 mg/ml), and GFP + cells were collected using a FACS. Total RNA was purified from pelleted cells (typically ,0.56106 GFP + events per retina).
Control retina development study 2 (Nrl-EGFP; P6)
Retinal rod cells isolated at postnatal day 6 from Nrl-EGFP mouse strain. Mice were sacrificed by decapitation 2–3 hours subsequent to light onset. Retinas from 4–5 independent biological replicates were immediately dissected from enucleated eyes, and dissociated with trypsin. Reactions were quenched with SBTI (1 mg/ml), and GFP + cells were collected using a FACS. Total RNA was purified from pelleted cells (typically ,0.56106 GFP + events per retina).

medulloblastoma study 2 (GCP) / cerebellar cell type study 2 (NSC)

Relative Expression (log2-ratio):-2.054389
Number of Samples:5 / 5
Experimental medulloblastoma study 2 (GCP)
Transfected medulloblastoma tumor cells isolated from the cerebellum of immunocompromised NSG mouse. This medulloblastoma was formed from transfected granule cell precursors (GCP), which were obtained from 5-7 days old mice of B6.129S-Atoh1tm4.1Hzo/J mouse strain. GCP were isolated using FACS based on their GFP expression. GCP were infected with Myc (stabilized form MycT58A) and DNp53 (p53 isoform that lacks 39 residues at the N-terminus) retroviruses for 20 hr and transplanted into NSG mice (6-8 weeks old). Tumor cells were isolated when the mice showed symptoms of medulloblastomas.
Control cerebellar cell type study 2 (NSC)
Cerebellar neural stem cells (NSC) isolated from 5-7 days old mice of C57BL/6J mouse strain. NSC were isolated using FACS based on the immunostaining for phycoerythrin-conjugated anti-Prominin1 (CD133) and lineage-negative (PSA-NCAM, O4, CD81) antibodies.

medulloblastoma study 2 (NSC) / cerebellar cell type study 2 (NSC)

Relative Expression (log2-ratio):-2.0231104
Number of Samples:5 / 5
Experimental medulloblastoma study 2 (NSC)
Transfected medulloblastoma tumor cells isolated from the cerebellum of immunocompromised NSG mouse. This medulloblastoma was formed from transfected cerebellar neural stem cells (NCS), which were obtained from 5-7 days old mice of C57BL/6J mouse strain. NSC were isolated using FACS based on the immunostaining for phycoerythrin-conjugated anti-Prominin1 (CD133) and lineage-negative (PSA-NCAM, O4, CD81) antibodies. NCS were infected with Myc (stabilized form MycT58A) and DNp53 (p53 isoform that lacks 39 residues at the N-terminus) retroviruses for 20 hr and transplanted into NSG mice (6-8 weeks old). Tumor cells were isolated when the mice showed symptoms of medulloblastomas.
Control cerebellar cell type study 2 (NSC)
Cerebellar neural stem cells (NSC) isolated from 5-7 days old mice of C57BL/6J mouse strain. NSC were isolated using FACS based on the immunostaining for phycoerythrin-conjugated anti-Prominin1 (CD133) and lineage-negative (PSA-NCAM, O4, CD81) antibodies.

burn injury study 2 (1d; splenocyte) / sham treated splenocyte sample (C57BL/6J; 1d)

Relative Expression (log2-ratio):-2.0205088
Number of Samples:4 / 4
Experimental burn injury study 2 (1d; splenocyte)
Splenocytes isolated from wild type males of C57BL/6J genetic background at 1st day after burn injury.
Control sham treated splenocyte sample (C57BL/6J; 1d)
Splenocytes at 1st day after sham burn injury.

bone marrow cell differentiation study 1 (Ebf2+) / bone marrow cell differentiation study 1 (Ebf2-)

Relative Expression (log2-ratio):1.9978285
Number of Samples:3 / 3
Experimental bone marrow cell differentiation study 1 (Ebf2+)
Adult freshly sorted Ebf2+ expressing bone marrow cells purified from bitransgenic Tg(Ebf2-CreERT2 X Rosa26-Yfp) mice. Expression of Ebf2 transcription factor was achieved by intraperitoneal injection of 4mg tamoxifen dissolved in corn oil for five consecutive days.
Control bone marrow cell differentiation study 1 (Ebf2-)
Adult freshly sorted Ebf2- bone marrow cells purified from bitransgenic Tg(Ebf2-CreERT2 X Rosa26-Yfp) mice. Mice were not treated with tamoxifen, thus Ebf2 gene was not expressed.

rosiglitazone study 3 (72h; UAMS-33/g2) / rosiglitazone study 3 (72h; UAMS-33/c)

Relative Expression (log2-ratio):-1.891201
Number of Samples:2 / 2
Experimental rosiglitazone study 3 (72h; UAMS-33/g2)
U-33/g2 cells treated with 1 μM rosiglitazone for 72 hours. ATC code:
Control rosiglitazone study 3 (72h; UAMS-33/c)
U-33/c cells treated with 1 μM rosiglitazone for 72 hours. ATC code:

testis development study 7 (P35) / testis development study 7 (P3)

Relative Expression (log2-ratio):-1.8757153
Number of Samples:2 / 2
Experimental testis development study 7 (P35)
Testis tissue isolated from wildtype mice at postnatal day P35.
Control testis development study 7 (P3)
Testis tissue isolated from wildtype mice at postnatal day P3.

retina development study 2 (Nrl-EGFP; P14) / retina development study 2 (Nrl-EGFP; P9)

Relative Expression (log2-ratio):1.8168268
Number of Samples:3 / 4
Experimental retina development study 2 (Nrl-EGFP; P14)
Retinal rod cells isolated at postnatal day 14 from Nrl-EGFP mouse strain. Mice were sacrificed by decapitation 2–3 hours subsequent to light onset. Retinas from 4–5 independent biological replicates were immediately dissected from enucleated eyes, and dissociated with trypsin. Reactions were quenched with SBTI (1 mg/ml), and GFP + cells were collected using a FACS. Total RNA was purified from pelleted cells (typically ,0.56106 GFP + events per retina).
Control retina development study 2 (Nrl-EGFP; P9)
Retinal rod cells isolated at postnatal day 9 from Nrl-EGFP mouse strain. Mice were sacrificed by decapitation 2–3 hours subsequent to light onset. Retinas from 4–5 independent biological replicates were immediately dissected from enucleated eyes, and dissociated with trypsin. Reactions were quenched with SBTI (1 mg/ml), and GFP + cells were collected using a FACS. Total RNA was purified from pelleted cells (typically ,0.56106 GFP + events per retina).

retina development study 2 (rds:Nrl-EGFP; P9) / retina development study 2 (Nrl-EGFP; P9)

Relative Expression (log2-ratio):1.7577009
Number of Samples:4 / 4
Experimental retina development study 2 (rds:Nrl-EGFP; P9)
Retinal rod cells isolated at postnatal day 9 from rds:Nrl-EGFP mouse strain - a model of slow retinal degenerations caused by spontaneous mutation in rds gene. Mice were sacrificed by decapitation 2–3 hours subsequent to the light onset. Retinas from 4–5 independent biological replicates were immediately dissected from enucleated eyes, and dissociated with trypsin. Reactions were quenched with SBTI (1 mg/ml), and GFP + cells were collected using a FACS. Total RNA was purified from pelleted cells (typically ,0.56106 GFP + events per retina).
Control retina development study 2 (Nrl-EGFP; P9)
Retinal rod cells isolated at postnatal day 9 from Nrl-EGFP mouse strain. Mice were sacrificed by decapitation 2–3 hours subsequent to light onset. Retinas from 4–5 independent biological replicates were immediately dissected from enucleated eyes, and dissociated with trypsin. Reactions were quenched with SBTI (1 mg/ml), and GFP + cells were collected using a FACS. Total RNA was purified from pelleted cells (typically ,0.56106 GFP + events per retina).