TOP TEN perturbations for P43267 (Mus musculus)

Organism: Mus musculus
Gene: P43267
Selected probe(set): 1452017_at
Platform: Affymetrix Mouse Genome 430 2.0 Array

Expression of P43267 (1452017_at) across 2697 perturbations tested by GENEVESTIGATOR:

tracheal epithelium study 1 (mixed cell pop.) / tracheal epithelium study 1 (non-basal cell pop.)

Relative Expression (log2-ratio):3.160564
Number of Samples:2 / 3
Experimental tracheal epithelium study 1 (mixed cell pop.)
KRT5-GFP negative and lectin positive tracheal epithelial cells population (basal and dendritic cells) isolated by FACS from KRT5-GFP transgenic mice. Cells were first sorted on the basis of binding of fluorescently labeled Griffonia simplicifolia isolectin A3B (GSI-A3B), which binds basal cells and a population of dendritic cells. The lectin positive cells were further sorted based on GFP expression. GFP is under the control of a KRT5 promoter, which is specifically active in basal cells.
Control tracheal epithelium study 1 (non-basal cell pop.)
KRT5-GFP negative and lectin negative tracheal epithelial cells population (non-basal cells) isolated by FACS from KRT5-GFP transgenic mice. Cells were first sorted on the basis of binding of fluorescently labeled Griffonia simplicifolia isolectin A3B (GSI-A3B), which binds basal cells and a population of dendritic cells. The lectin negative cells were further sorted based on GFP expression. GFP is under the control of a KRT5 promoter, which is specifically active in basal cells.

RhoA knockout study 1 (in vivo) / RhoA knockout study 1 (in vitro)

Relative Expression (log2-ratio):-2.352952
Number of Samples:2 / 3
Experimental RhoA knockout study 1 (in vivo)
Freshly isolated RhoA-null keratinocytes obtained from epidermis of RhoA (fl/fl) K5 Cre mice. Skin samples were washed for 5 min in PBS containing antibiotics followed by digestion with 0.8% trypsin in PBS (50 min; 37 °C). Obtained epidermis was shaken for 30 min in low-calcium MEM supplemented with 0.25 mg/ml DNAase I and 8 % FCS treated with Chelex. Cell suspension was filtered (70 um) and collected keratinocytes were washed once with DNase I medium, resuspended and analyzed. RhoA: Ras homolog gene family, member A.
Control RhoA knockout study 1 (in vitro)
Cultured primary RhoA-null keratinocytes obtained from epidermis of RhoA (fl/fl) K5 Cre mice. Primary keratinocytes were cultured in low-calcium MEM containing 5 ug/ml insulin, 10 ng/ml epidermal growth factor, 10 ug/ml transferrin, 10 uM phosphoethanolamine, 10 uM ethanolamine, 0.36 ug/ml hydrocortisone, 2 mM L-glutamine, 100 U/ml penicillin, 100 ug/ml streptomycin and 8% Chelex-treated FCS. RhoA: Ras homolog gene family, member A.

wound healing study 1 (12h; skin) / unwounded skin tissue (BALB/c)

Relative Expression (log2-ratio):2.173068
Number of Samples:3 / 3
Experimental wound healing study 1 (12h; skin)
Skin wound tissue isolated 12 hours after injury from 6-8 weeks old females of BALB/c strain. Skin wounds include epidermis, dermis, subcutaneous, and muscle.
Control unwounded skin tissue (BALB/c)
Dorsal skin tissue isolated from uninjured tissue from 6-8 weeks old females of BALB/c strain.

wound healing study 1 (24h; skin) / unwounded skin tissue (BALB/c)

Relative Expression (log2-ratio):2.0030994
Number of Samples:3 / 3
Experimental wound healing study 1 (24h; skin)
Skin wound tissue isolated 24 hours after injury from 6-8 weeks old females of BALB/c strain. Skin wounds include epidermis, dermis, subcutaneous, and muscle.
Control unwounded skin tissue (BALB/c)
Dorsal skin tissue isolated from uninjured tissue from 6-8 weeks old females of BALB/c strain.

oogenesis study 1 (Lhx8-/-) / normal ovary tissue (B6129S6)

Relative Expression (log2-ratio):-1.4317818
Number of Samples:3 / 2
Experimental oogenesis study 1 (Lhx8-/-)
Ovary tissue isolated from neonate Lhx8-/- females at 0.5 postnatal day of their development. Mutant females were on hybrid C57BL/6 X 129S6/SvEv genetic background.
Control normal ovary tissue (B6129S6)
Ovary tissue isolated from neonate females at 0.5 postnatal day of their development. Females were on hybrid C57BL/6 X 129S6/SvEv genetic background.

testis development study 7 (P30) / testis development study 7 (P10)

Relative Expression (log2-ratio):1.3169994
Number of Samples:2 / 2
Experimental testis development study 7 (P30)
Testis tissue isolated from wildtype mice at postnatal day P30.
Control testis development study 7 (P10)
Testis tissue isolated from wildtype mice at postnatal day P10.

skin inflammation study 1 (IL-23; PBS) / normal dorsal skin tissue (C57BL/6J)

Relative Expression (log2-ratio):1.3016396
Number of Samples:5 / 5
Experimental skin inflammation study 1 (IL-23; PBS)
Samples of mock treated psoriasis-like skin lesions induced by intradermal injection of IL-23. Skin inflammation was induced in seven weeks old C57BL/6 mice by intradermal injection of recombinant murine IL-23 (3ug in 100ul PBS) applied for four consecutive days (day 1-4). Mice were further mock treated subcutaneously with 100ul of PBS on day 4, 5 and 6. Samples were taken at day 7.
Control normal dorsal skin tissue (C57BL/6J)
Samples of dorsal skin tissue taken from 8 weeks old C57BL/6J mice.

wound healing study 1 (3d; skin) / unwounded skin tissue (BALB/c)

Relative Expression (log2-ratio):1.2758579
Number of Samples:3 / 3
Experimental wound healing study 1 (3d; skin)
Skin wound tissue isolated 3 days after injury from 6-8 weeks old females of BALB/c strain. Skin wounds include epidermis, dermis, subcutaneous, and muscle.
Control unwounded skin tissue (BALB/c)
Dorsal skin tissue isolated from uninjured tissue from 6-8 weeks old females of BALB/c strain.

wound healing study 1 (6h; skin) / unwounded skin tissue (BALB/c)

Relative Expression (log2-ratio):1.2587919
Number of Samples:3 / 3
Experimental wound healing study 1 (6h; skin)
Skin wound tissue isolated 6 hours after injury from 6-8 weeks old females of BALB/c strain. Skin wounds include epidermis, dermis, subcutaneous, and muscle.
Control unwounded skin tissue (BALB/c)
Dorsal skin tissue isolated from uninjured tissue from 6-8 weeks old females of BALB/c strain.

testis development study 7 (P30) / testis development study 7 (P6)

Relative Expression (log2-ratio):1.2531652
Number of Samples:2 / 2
Experimental testis development study 7 (P30)
Testis tissue isolated from wildtype mice at postnatal day P30.
Control testis development study 7 (P6)
Testis tissue isolated from wildtype mice at postnatal day P6.