TOP TEN perturbations for Q3USZ2 (Mus musculus)

Organism: Mus musculus
Gene: Q3USZ2
Selected probe(set): 1439670_at
Platform: Affymetrix Mouse Genome 430 2.0 Array

Expression of Q3USZ2 (1439670_at) across 2697 perturbations tested by GENEVESTIGATOR:

parthenogenic embryos study 1 (1 cell; B6D2) / in vivo fertilized embryo (1 cell) sample (B6D2)

Relative Expression (log2-ratio):2.686904
Number of Samples:4 / 4
Experimental parthenogenic embryos study 1 (1 cell; B6D2)
Diploid parthenogenetically activated 1 cell embryos obtained from B6D2 females.
Control in vivo fertilized embryo (1 cell) sample (B6D2)
In vivo (1 cell) fertilized embryos obtained by mating (B6D2)F1 mice after injection of females 8–12 weeks of age with Pregnant Mare Serum Gonadotropin (PMSG) and human Chorionic Gonadotrophin (hCG).

ESC study 3 (Nanog) / ESC study 3 (empty vector)

Relative Expression (log2-ratio):2.419918
Number of Samples:5 / 5
Experimental ESC study 3 (Nanog)
Mouse embryonic stem cells were isolated 3 days after transfection with constructs expressing Nanog.
Control ESC study 3 (empty vector)
Mouse embryonic stem cells were isolated 3 days after transfection with constructs expressing empty vector.

ESC study 3 (Pouf5f1) / ESC study 3 (Nanog)

Relative Expression (log2-ratio):-2.3931475
Number of Samples:4 / 5
Experimental ESC study 3 (Pouf5f1)
Mouse embryonic stem cells were isolated 3 days after transfection with constructs expressing Pouf5f1.
Control ESC study 3 (Nanog)
Mouse embryonic stem cells were isolated 3 days after transfection with constructs expressing Nanog.

somatic cell nuclear transfer study 1 / parthenogenic embryos study 1 (1 cell; B6D2)

Relative Expression (log2-ratio):-1.9164963
Number of Samples:4 / 4
Experimental somatic cell nuclear transfer study 1
Cloned embryos (1 cell) produced by somatic cell nuclear transfer (SCNT), cultured without a-amanitin. For SCNT, adherent adult cumulus cells (presumably G1 phase) from ovulated oocytes were employed as nuclear donors.
Control parthenogenic embryos study 1 (1 cell; B6D2)
Diploid parthenogenetically activated 1 cell embryos obtained from B6D2 females.

somatic cell nuclear transfer study 2 / in vivo fertilized embryo (2 cell) sample (B6D2)

Relative Expression (log2-ratio):-1.0227604
Number of Samples:4 / 4
Experimental somatic cell nuclear transfer study 2
Cloned embryos (2 cell) produced by somatic cell nuclear transfer (SCNT), cultured without a-amanitin. For SCNT, adherent adult cumulus cells (presumably G1 phase) from ovulated oocytes were employed as nuclear donors.
Control in vivo fertilized embryo (2 cell) sample (B6D2)
In vivo (2 cell) fertilized embryos obtained by mating (B6D2)F1 mice after injection of females 8-12 weeks of age with Pregnant Mare Serum Gonadotropin (PMSG) and human Chorionic Gonadotrophin (hCG).

smoking study 1 (8d) / mock treated lung tissue (A/J; 8d)

Relative Expression (log2-ratio):-0.94746685
Number of Samples:3 / 3
Experimental smoking study 1 (8d)
Lung samples of male A/J mice. Mice were smoke exposed 7 hours/day for 8 days by burning 2R4F reference cigarettes (2.45 mg nicotine per cigarette) using a smoking machine. Each smoldering cigarette was puffed for 2 seconds, once every minute for a total of 8 puffs, at a flow rate of 1.05 l/min, to provide a standard puff of 35 cm3. The smoke machine was adjusted to produce a mixture of sidestream smoke (89%) and mainstream smoke (11%) by burning five cigarettes at one time. The total particulate matter in the exposure chambers was on an average 90 mg/m3, and carbon monoxide concentration was 350 ppm.
Control mock treated lung tissue (A/J; 8d)
Lung tissue isolated from control A/J males that were 8 days exposed to filtered air environment.

somatic cell nuclear transfer study 1 / in vivo fertilized embryo (1 cell) sample (B6D2)

Relative Expression (log2-ratio):0.7704077
Number of Samples:4 / 4
Experimental somatic cell nuclear transfer study 1
Cloned embryos (1 cell) produced by somatic cell nuclear transfer (SCNT), cultured without a-amanitin. For SCNT, adherent adult cumulus cells (presumably G1 phase) from ovulated oocytes were employed as nuclear donors.
Control in vivo fertilized embryo (1 cell) sample (B6D2)
In vivo (1 cell) fertilized embryos obtained by mating (B6D2)F1 mice after injection of females 8–12 weeks of age with Pregnant Mare Serum Gonadotropin (PMSG) and human Chorionic Gonadotrophin (hCG).

somatic cell nuclear transfer study 2 / parthenogenic embryos study 1 (2 cell; B6D2)

Relative Expression (log2-ratio):-0.76183605
Number of Samples:4 / 4
Experimental somatic cell nuclear transfer study 2
Cloned embryos (2 cell) produced by somatic cell nuclear transfer (SCNT), cultured without a-amanitin. For SCNT, adherent adult cumulus cells (presumably G1 phase) from ovulated oocytes were employed as nuclear donors.
Control parthenogenic embryos study 1 (2 cell; B6D2)
Diploid parthenogenetically activated 2 cell embryos obtained from B6D2 females.

placenta development study 1 (fetal placenta; E19) / placenta development study 1 (fetal placenta; E8.5)

Relative Expression (log2-ratio):0.6988764
Number of Samples:2 / 5
Experimental placenta development study 1 (fetal placenta; E19)
Placenta tissue (fetal part/origin) isolated from wild type embryos (Swiss Webster) at E19 developmental stage.
Control placenta development study 1 (fetal placenta; E8.5)
Placenta tissue (fetal part/origin) isolated from wild type embryos (Swiss Webster) at E8.5 developmental stage.

BMP2 study 1 (24h) / mock treated neural precursor cell sample (24h; C57BL/6J)

Relative Expression (log2-ratio):0.5760522
Number of Samples:2 / 6
Experimental BMP2 study 1 (24h)
Neural precursors obtained from 15.5 days old embryonic medial and lateral ganglionic eminences and treated with BMP2 for 6 hours. Neural precursors were cultured as neurospheres for 7 days, then they were mechanically dissociated and plated on poly-ornithine coated petri dish. 36 hours after plating, cells were treated for 24 hours with 10 ng/ml BMP2 in 1 mM Hepes, 0.1% FCS and 1 g/ml glucose.
Control mock treated neural precursor cell sample (24h; C57BL/6J)
Neural precursors obtained from 15. 5 days old embryonic medial and lateral ganglionic eminences and treated with vehicle for 24 hours. Neural precursors were cultured as neurospheres for 7 days, then they were mechanically dissociated and plated on poly-ornithine coated petri dish. 36 hours after plating, cells were treated for 24 hours with 1 mM Hepes, 0.1% FCS and 1 g/ml glucose.