TOP TEN perturbations for Q66X19 (Mus musculus)

Organism: Mus musculus
Gene: Q66X19
Selected probe(set): 1457179_at
Platform: Affymetrix Mouse Genome 430 2.0 Array

Expression of Q66X19 (1457179_at) across 2667 perturbations tested by GENEVESTIGATOR:

somatic cell nuclear transfer study 2 / parthenogenic embryos study 1 (2 cell; B6D2)

Relative Expression (log2-ratio):-2.8339977
Number of Samples:4 / 4
Experimental somatic cell nuclear transfer study 2
Cloned embryos (2 cell) produced by somatic cell nuclear transfer (SCNT), cultured without a-amanitin. For SCNT, adherent adult cumulus cells (presumably G1 phase) from ovulated oocytes were employed as nuclear donors.
Control parthenogenic embryos study 1 (2 cell; B6D2)
Diploid parthenogenetically activated 2 cell embryos obtained from B6D2 females.

parthenogenic embryos study 1 (2 cell; B6D2) / in vivo fertilized embryo (2 cell) sample (B6D2)

Relative Expression (log2-ratio):2.448203
Number of Samples:4 / 4
Experimental parthenogenic embryos study 1 (2 cell; B6D2)
Diploid parthenogenetically activated 2 cell embryos obtained from B6D2 females.
Control in vivo fertilized embryo (2 cell) sample (B6D2)
In vivo (2 cell) fertilized embryos obtained by mating (B6D2)F1 mice after injection of females 8-12 weeks of age with Pregnant Mare Serum Gonadotropin (PMSG) and human Chorionic Gonadotrophin (hCG).

folliculogenesis study 1 (Pik3cd +/-; PMSG) / normal ovary tissue (Pik3cd +/-)

Relative Expression (log2-ratio):-1.9422626
Number of Samples:3 / 3
Experimental folliculogenesis study 1 (Pik3cd +/-; PMSG)
Ovary tissue samples isolated from females carrying monoallelic mutation in Pik3cd gene. These female mice contained one allele of Pik3cd gene disrupted by gene trap cassette and one functional wild type allele. Presence of wild type allele leads to normal Pik3cd expression in ovaries. Mice were injected with 5IU pregnant mare’s serum gonadotropin (PMSG) and samples were harvested 44 hours after injection. Mice were mixed background of 129S5 X C57BL/6J and were sacrificed at 3th week of their age.
Control normal ovary tissue (Pik3cd +/-)
Ovary tissue samples isolated from females of B6.129S5-Pik3cdGt(OST451938)Lex strain. These female mice contained one allele of Pik3cd gene disrupted by gene trap cassette and one functional wild type allele. Presence of wild type allele leads to normal Pik3cd expression in ovaries. Mice were mixed background of 129S5 X C57BL/6J and were sacrificed at 3th week of their age.

somatic cell nuclear transfer study 2 (a-amanitin) / in vivo fertilized embryo (2 cell) sample (B6D2; a-amanitin)

Relative Expression (log2-ratio):-1.8439989
Number of Samples:4 / 4
Experimental somatic cell nuclear transfer study 2 (a-amanitin)
Cloned embryos (2 cell) produced by somatic cell nuclear transfer (SCNT), cultured with a-amanitin. For SCNT, adherent adult cumulus cells (presumably G1 phase) from ovulated oocytes were employed as nuclear donors.
Control in vivo fertilized embryo (2 cell) sample (B6D2; a-amanitin)
In vivo fertilized embryos (2 cell), obtained by mating (B6D2)F1 mice after injection of females 8-12 weeks of age with Pregnant Mare Serum Gonadotropin (PMSG) and human Chorionic Gonadotrophin (hCG). Embryos were cultured in KSOM media with a-amanitin.

Ccna2 depletion study 1 / normal embryonic cell sample (Ccna2; B6D2)

Relative Expression (log2-ratio):1.1190262
Number of Samples:3 / 3
Experimental Ccna2 depletion study 1
Mid-2-cell stage embryos injected with Ccna2 antisense morpholino oligonucleotides (MO) to obtain (MO)-mediated Ccna2 gene knockdown. 1-cell embryos were isolated from mouse females oviducts. Cumulus cells were removed by hyaluronidase treatment and pipetting. Pre-implantation embryos at the two pronuclei stage were recovered, pooled from 3-6 females in M2 media, followed by immediate cytoplasmic microinjection of gene-specific Ccna2 antisense morpholino oligonucleotides and cultured in Human Tubal Fluid with 10% serum supplement. Samples containing 20 pooled injected mouse embryos were washed through 3 drops of PBS/PVP and collected for total RNA extraction.
Control normal embryonic cell sample (Ccna2; B6D2)
Untreated mid-2-cell stage embryos isolated from mouse females oviducts. Samples containing 20 pooled control mouse embryos (from 3-6 females) were washed through 3 drops of PBS/PVP and collected for total RNA extraction. These control samples were processed simultaneously with embryos treated with Ccna2 antisense morpholino oligonucleotides.

MRL/MpJ / AKR/N

Relative Expression (log2-ratio):-1.0382481
Number of Samples:2 / 2
Experimental MRL/MpJ
The MRL/MpJ wildtype strain was generated from a series of crosses with strains LG/J (75%), AKR/J (12.6%), C3H/HeDi (12.1%), and C57BL/6J (0.3%), and then followed by inbreeding. It has been reported that the MRL/MpJ mouse strain shows several unique phenotypes, including inherent collagen disease, rapid wound healing, heat shock-resistant spermatocytes, and metaphase-specific apoptosis in the testis.
Control AKR/N

Smith-Magenis syndrome study 1 (testis) / Potocki-Lupski syndrome study 1 (testis)

Relative Expression (log2-ratio):1.0308199
Number of Samples:3 / 3
Experimental Smith-Magenis syndrome study 1 (testis)
Whole testis from adult mouse of Df(11)17/+ strain (animal model of Smith-Magenis). This mouse strain carries a deletion at band MMU11B2 on chromosome 11. This region encompasses a chromosomal segment that shares conserved synteny with the Smith-Magenis and Potocki-Lupski syndrome critical interval on human Chromosome 17.
Control Potocki-Lupski syndrome study 1 (testis)
Whole testis from adult mouse of Dp(11)17/+ strain (animal model of Potocki-Lupski). This mouse strain carries a duplication at band MMU11B2 on chromosome 11. This region encompasses a chromosomal segment that shares conserved synteny with the Potocki-Lupski critical interval on human Chromosome 17.

folliculogenesis study 1 (Pik3cd +/-; E2) / normal ovary tissue (Pik3cd +/-)

Relative Expression (log2-ratio):-1.0019608
Number of Samples:3 / 3
Experimental folliculogenesis study 1 (Pik3cd +/-; E2)
Ovary tissue samples isolated from females carrying monoallelic mutation in Pik3cd gene. These female mice contained one allele of Pik3cd gene disrupted by gene trap cassette and one functional wild type allele. Presence of wild type allele leads to normal Pik3cd expression in ovaries. Mice were injected with 60ul of the 17-beta-estradiol (E2) solution 3 times per 24 hours, and ovaries were dissected 72 hours after initial injection.
Control normal ovary tissue (Pik3cd +/-)
Ovary tissue samples isolated from females of B6.129S5-Pik3cdGt(OST451938)Lex strain. These female mice contained one allele of Pik3cd gene disrupted by gene trap cassette and one functional wild type allele. Presence of wild type allele leads to normal Pik3cd expression in ovaries. Mice were mixed background of 129S5 X C57BL/6J and were sacrificed at 3th week of their age.

testis development study 4 (E16) / testis development study 4 (E11)

Relative Expression (log2-ratio):0.8193097
Number of Samples:3 / 5
Experimental testis development study 4 (E16)
Whole testis tissue isolated from male embryos of CD-1 genetic background at developmental stage E16.
Control testis development study 4 (E11)
Whole testis tissue isolated from male embryos of CD-1 genetic background at developmental stage E11.

cecal ligation and puncture study 1 (late; thrombocyte) / cecal ligation and puncture study 1 (early; thrombocyte)

Relative Expression (log2-ratio):-0.7752991
Number of Samples:2 / 2
Experimental cecal ligation and puncture study 1 (late; thrombocyte)
Platelets isolated from BALB/c mice by using a negative selection technique, 48 hours after cecal ligation and puncture (CLP) treatment. CLP is a widely used experimental model of sepsis.
Control cecal ligation and puncture study 1 (early; thrombocyte)
Platelets isolated from BALB/c mice by using a negative selection technique, 24 hours after cecal ligation and puncture (CLP) treatment. CLP is a widely used experimental model of sepsis.