TOP TEN perturbations for Q8C9E8 (Mus musculus)

Organism: Mus musculus
Gene: Q8C9E8
Selected probe(set): 1436576_at
Platform: Affymetrix Mouse Genome 430 2.0 Array

Expression of Q8C9E8 (1436576_at) across 2604 perturbations tested by GENEVESTIGATOR:

lipopolysaccharide study 4 (late; peritoneal macrophage) / untreated thioglycollate-elicited peritoneal macrophage sample (C57Bl/6)

Relative Expression (log2-ratio):7.8039494
Number of Samples:2 / 2
Experimental lipopolysaccharide study 4 (late; peritoneal macrophage)
Thioglycollate-elicited peritoneal macrophages harvested from the peritoneal cavity of C57Bl/6 mice 3 days after intra-peritoneal administration of 3% thioglycollate broth. Cells were then cultured for 2 hours and non-adherent cells were removed by washing with PBS. Adherent cells were then treated with lipopolysaccharide for 7 hours. ATC code:---
Control untreated thioglycollate-elicited peritoneal macrophage sample (C57Bl/6)
Thioglycollate-elicited peritoneal macrophages harvested from the peritoneal cavity of C57Bl/6 mice 3 days after intra-peritoneal administration of 3% thioglycollate broth. Cells were then cultured for 2 hours and non-adherent cells were removed by washing with PBS. Adherent cells were then left untreated, and RNA was extracted.

interferon gamma study 1 (Atg5 flox/flox) / macrophage study 7 (Atg5 flox/flox)

Relative Expression (log2-ratio):6.1411324
Number of Samples:2 / 2
Experimental interferon gamma study 1 (Atg5 flox/flox)
Bone marrow derived macrophages (BMDM) from non-wildtype mouse strain (Atg5 flox/flox) treated with 100 U/ml of interferon gamma for 14 hours. BMDM were generated by isolating and culturing mouse bone marrows on non-tissue-culture plate for 7 days in BMDM media, 10% fetal bovine serum, 5% horse serum, 10% CMG14-12, 1x MEM nonessential amino acids, 1mM sodium pyruvate, 2mM L-glutamine. After 7 days BMDM were dissociated from the plate and set up for the treatment experiment. ATC code:
Control macrophage study 7 (Atg5 flox/flox)
Bone marrow derived macrophages (BMDM) from non-wildtype mouse strain (Atg5 flox/flox). BMDM were generated by isolating and culturing mouse bone marrows on non-tissue-culture plate for 7 days in BMDM media, 10% fetal bovine serum, 5% horse serum, 10% CMG14-12, 1x MEM nonessential amino acids, 1 mM sodium pyruvate, 2 mM L-glutamine.

M. tuberculosis study 1 (TNF-alpha -/-; inf.; saline) / M. tuberculosis study 1 (TNF-alpha -/-; uninf.; saline)

Relative Expression (log2-ratio):6.0482006
Number of Samples:4 / 3
Experimental M. tuberculosis study 1 (TNF-alpha -/-; inf.; saline)
Lung samples obtained from M. tuberculosis H37Rv infected TNF-alpha -/- mouse strain on a mock (saline) treatment. One day before the infection with M. tuberculosis, mice (age at 8 - 12 weeks, female) were treated with saline. Subsequent saline treatment was applied in weekly intervals into infected mice. Pulmonary infection with M. tuberculosis H37Rv was performed under xylazine-ketamine anesthesia by delivering 20 ul of bacterial inoculum (1000±300 CFU) into each nostril. Twenty four hours post infection, the bacterial dose was confirmed in the lungs of 2 wild-type control mice. The experiment was terminated 1 month after M. tuberculosis inoculation and lung samples were processed for microarray analysis.
Control M. tuberculosis study 1 (TNF-alpha -/-; uninf.; saline)
Lung samples from non-infected TNF-alpha -/- mouse strain obtained after 1 month of mock (saline) treatment in weekly intervals.

interferon gamma study 1 (Atg5 flox/flox+LysM cre) / macrophage study 7 (Atg5 flox/flox+LysM cre)

Relative Expression (log2-ratio):5.7590313
Number of Samples:2 / 2
Experimental interferon gamma study 1 (Atg5 flox/flox+LysM cre)
Bone marrow derived macrophages (BMDM) from non-wildtype mouse strain (Atg5 flox/flox+LysM cre, with Atg5-deficient macrophages) treated with 100 U/ml of interferon gamma for 14 hours. BMDM were generated by isolating and culturing mouse bone marrows on non-tissue-culture plate for 7 days in BMDM media, 10% fetal bovine serum, 5% horse serum, 10% CMG14-12, 1x MEM nonessential amino acids, 1 mM sodium pyruvate, 2 mM L-glutamine. After 7 days BMDM were dissociated from the plate and set up for the treatment experiment. ATC code:
Control macrophage study 7 (Atg5 flox/flox+LysM cre)
Bone marrow derived macrophages (BMDM) from non-wildtype mouse strain (Atg5 flox/flox+LysM cre, with Atg5-deficient macrophages). BMDM were generated by isolating and culturing mouse bone marrows on non-tissue-culture plate for 7 days in BMDM media, 10% fetal bovine serum, 5% horse serum, 10% CMG14-12, 1x MEM nonessential amino acids, 1 mM sodium pyruvate, 2 mM L-glutamine.

IFNg study 1 / untreated bone marrow derived macrophage cell sample (C57BL/6J)

Relative Expression (log2-ratio):5.647229
Number of Samples:4 / 4
Experimental IFNg study 1
Bone marrow derived macrophages (BMDMs) harvested 24 hours after treatment with IFN-gamma (10 ng/ml). Briefly, BMDMs were obtained from femur and tibia bone marrows of 3 – 5 wild-type C57BL/6 mice. Bone marrow cells were flushed, erythrocytes were removed by ACK lysis and filtering. Additionally, the single-cell suspension was cultured for 6 days in complete DMEM with 20% L929 conditioned media or 50ng/ml mouse recombinant M-CSF to obtain fully differentiated BMDMs. BMDMs were confirmed by FACS analysis as CD11b+/F4/80+ cells. ATC code:
Control untreated bone marrow derived macrophage cell sample (C57BL/6J)
Bone marrow derived macrophages obtained from femur and tibia bone marrows of 3 – 5 wild-type C57BL/6 mice. Bone marrow cells were flushed, erythrocytes were removed by ACK lysis and filtering. Additionally, the single-cell suspension was cultured for 6 days in complete DMEM with 20% L929 conditioned media or 50ng/mL mouse recombinant M-CSF to obtain fully differentiated BMDMs. BMDMs were confirmed by FACS analysis as CD11b+/F4/80+ cells.

IFNg; LPS study 1 / untreated bone marrow derived macrophage cell sample (C57BL/6J)

Relative Expression (log2-ratio):5.3274393
Number of Samples:4 / 4
Experimental IFNg; LPS study 1
Bone marrow derived macrophages (BMDMs) harvested 24 hours after treatment with IFN-gamma (10 ng/ml) and lipopolysaccharide (10 ng/ml). Briefly, BMDMs were obtained from femur and tibia bone marrows of 3 – 5 wild-type C57BL/6 mice. Bone marrow cells were flushed, erythrocytes were removed by ACK lysis and filtering. Additionally, the single-cell suspension was cultured for 6 days in complete DMEM with 20% L929 conditioned media or 50ng/ml mouse recombinant M-CSF to obtain fully differentiated BMDMs. BMDMs were confirmed by FACS analysis as CD11b+/F4/80+ cells. ATC code:
Control untreated bone marrow derived macrophage cell sample (C57BL/6J)
Bone marrow derived macrophages obtained from femur and tibia bone marrows of 3 – 5 wild-type C57BL/6 mice. Bone marrow cells were flushed, erythrocytes were removed by ACK lysis and filtering. Additionally, the single-cell suspension was cultured for 6 days in complete DMEM with 20% L929 conditioned media or 50ng/mL mouse recombinant M-CSF to obtain fully differentiated BMDMs. BMDMs were confirmed by FACS analysis as CD11b+/F4/80+ cells.

lipopolysaccharide study 3 (intermediate; BMDM) / CSF-1 cultured bone marrow-derived macrophage sample (C57Bl/6)

Relative Expression (log2-ratio):5.0606003
Number of Samples:2 / 2
Experimental lipopolysaccharide study 3 (intermediate; BMDM)
Bone marrow-derived macrophages were prepared from bone marrow cells from femurs of adult C57Bl/6 mice by culturing for 7 days in the presence of 10 000 U/ml CSF-1. On day 7 cells were treated with 10 ng/mL Salmonella minnesota lipopolysaccharide (LPS; Re595 mutant) for 6 hours. ATC code:---
Control CSF-1 cultured bone marrow-derived macrophage sample (C57Bl/6)
Bone marrow-derived macrophages were prepared from bone marrow cells from femurs of adult C57Bl/6 mice by culturing for 7 days in the presence of 10 000 U/ml CSF-1, untreated.

M. tuberculosis study 1 (C57BL/6; inf.; saline) / M. tuberculosis study 1 (C57BL/6; uninf.; saline)

Relative Expression (log2-ratio):4.8296747
Number of Samples:10 / 9
Experimental M. tuberculosis study 1 (C57BL/6; inf.; saline)
Lung samples obtained from M. tuberculosis H37Rv infected C57BL/6 mouse strain on a mock (saline) treatment. One day before the infection with M. tuberculosis, mice (age at 8 - 12 weeks, female) were treated with saline. Subsequent saline treatment was applied in weekly intervals into infected mice. Pulmonary infection with M. tuberculosis H37Rv was performed under xylazine-ketamine anesthesia by delivering 20 ul of bacterial inoculum (1000±300 CFU) into each nostril. Twenty four hours post infection, the bacterial dose was confirmed in the lungs of 2 wild-type control mice. The experiment was terminated 1 month after M. tuberculosis inoculation and lung samples were processed for microarray analysis.
Control M. tuberculosis study 1 (C57BL/6; uninf.; saline)
Lung samples from non-infected wild-type C57BL/6 mouse strain obtained 1 month after the first treatment with saline. The treatment with saline was received in weekly intervals.

poly I:C study 1 (6h) / vehicle treated lung tissue (BALB/cJ; 6h)

Relative Expression (log2-ratio):4.8135786
Number of Samples:6 / 6
Experimental poly I:C study 1 (6h)
Lung tissues from BALB/cJ male mice treated for 6 hours with polyinosinic:polycytidylic acid (poly I:C). Poly I:C was administrated intranasally at a sub-maximal dose of 30 ug (in 50 ul sterile saline) after isoflurane anesthesia. At the time of experiment mice were 7-9 weeks old. Poly I:C is an analogue of double-stranded RNA and it simulates viral infection. The molecular responses in the airways induced by poly I:C correlate to those observed in the lungs of COPD patients. ATC code:
Control vehicle treated lung tissue (BALB/cJ; 6h)
Lung tissues from BALB/cJ male mice treated for 6 hours with saline. Vehicle (50 ul) was administrated intranasally after isoflurane anesthesia. At the time of experiment mice were 7-9 weeks old.

lipopolysaccharide study 5 (naive) / untreated macrophage sample (C57BL/6)

Relative Expression (log2-ratio):4.497986
Number of Samples:2 / 2
Experimental lipopolysaccharide study 5 (naive)
Macrophages were left untreated (Naive) for 24 hours, washed twice with warm PBS and given 10ng/ml LPS for 4 hours. ATC code:---
Control untreated macrophage sample (C57BL/6)
Macrophages were left untreated (Naive) for 24 hours, washed twice with warm PBS and given fresh media for 4 hours.