TOP TEN perturbations for Q8K088 (Mus musculus)

Organism: Mus musculus
Gene: Q8K088
Selected probe(set): 1455273_at
Platform: Affymetrix Mouse Genome 430 2.0 Array

Expression of Q8K088 (1455273_at) across 2700 perturbations tested by GENEVESTIGATOR:

retina development study 3 (P8; Ndrg+ amacrine cell) / retina development study 3 (P5; Ndrg+ amacrine cell)

Relative Expression (log2-ratio):4.5520563
Number of Samples:2 / 15
Experimental retina development study 3 (P8; Ndrg+ amacrine cell)
Retinal amacrine cells isolated based on their GFP fluorescence at postanatal day P8. In vivo transfection of pNdrg4::GFP reporter into the developing retina (P0) in the CD-1 mouse strain was done by electroporation. Retinal samples were harvested 4 days later from the developing retinas and amacrine cells were isolated due to their GFP expression pattern.
Control retina development study 3 (P5; Ndrg+ amacrine cell)
Retinal amacrine cells isolated based on their GFP fluorescence at postanatal day P5. In vivo transfection of pNdrg4::GFP reporter into the developing retina (P0) in the CD-1 mouse strain was done by electroporation. Retinal samples were harvested 4 days later from the developing retinas and amacrine cells were isolated due to their GFP expression pattern.

Purkinje cells study 1 (P35) / Purkinje cells study 1 (P3)

Relative Expression (log2-ratio):-2.150898
Number of Samples:4 / 3
Experimental Purkinje cells study 1 (P35)
Purkinje cells were isolated from CB6-Tg(Gad1-EGFP)G42Zjh/J mouse strain. These cells were manually sorted from the cerebellum of a 35-days-old mouse study 1 (P35) based on EGFP fluorescence, shape, size and localization. The numbers of cells were from 50 to 75.
Control Purkinje cells study 1 (P3)
Purkinje cells were isolated from CB6-Tg(Gad1-EGFP)G42Zjh/J mouse strain. These cells were manually sorted from the cerebellum of a 3-days-old mouse (P3) based on EGFP fluorescence, shape, size and localization. The numbers of cells were from 50 to 75.

somatic cell nuclear transfer study 2 / parthenogenic embryos study 1 (2 cell; B6D2)

Relative Expression (log2-ratio):2.082755
Number of Samples:4 / 4
Experimental somatic cell nuclear transfer study 2
Cloned embryos (2 cell) produced by somatic cell nuclear transfer (SCNT), cultured without a-amanitin. For SCNT, adherent adult cumulus cells (presumably G1 phase) from ovulated oocytes were employed as nuclear donors.
Control parthenogenic embryos study 1 (2 cell; B6D2)
Diploid parthenogenetically activated 2 cell embryos obtained from B6D2 females.

parthenogenic embryos study 1 (2 cell; B6D2) / in vivo fertilized embryo (2 cell) sample (B6D2)

Relative Expression (log2-ratio):-1.978509
Number of Samples:4 / 4
Experimental parthenogenic embryos study 1 (2 cell; B6D2)
Diploid parthenogenetically activated 2 cell embryos obtained from B6D2 females.
Control in vivo fertilized embryo (2 cell) sample (B6D2)
In vivo (2 cell) fertilized embryos obtained by mating (B6D2)F1 mice after injection of females 8-12 weeks of age with Pregnant Mare Serum Gonadotropin (PMSG) and human Chorionic Gonadotrophin (hCG).

Purkinje cells study 1 (P14) / Purkinje cells study 1 (P3)

Relative Expression (log2-ratio):-1.9720335
Number of Samples:3 / 3
Experimental Purkinje cells study 1 (P14)
Purkinje cells were isolated from CB6-Tg(Gad1-EGFP)G42Zjh/J mouse strain. These cells were manually sorted from the cerebellum of a 14-days-old mouse study 1 (P14) based on EGFP fluorescence, shape, size and localization. The numbers of cells were from 50 to 75.
Control Purkinje cells study 1 (P3)
Purkinje cells were isolated from CB6-Tg(Gad1-EGFP)G42Zjh/J mouse strain. These cells were manually sorted from the cerebellum of a 3-days-old mouse (P3) based on EGFP fluorescence, shape, size and localization. The numbers of cells were from 50 to 75.

retina development study 1 (P28; wt-GFP) / retina development study 1 (P2; wt-GFP)

Relative Expression (log2-ratio):1.943469
Number of Samples:4 / 4
Experimental retina development study 1 (P28; wt-GFP)
Retina tissue isolated at postanatal day 28 from wt-GFP mouse strain. This strain expresses EGFP under the control of Nrl gene promoter - a retina specific gene.
Control retina development study 1 (P2; wt-GFP)
Retina tissue isolated at postanatal day 2 from wt-GFP mouse strain. This strain expresses EGFP under the control of Nrl gene promoter - a retina specific gene.

Purkinje cells study 1 (P21) / Purkinje cells study 1 (P3)

Relative Expression (log2-ratio):-1.853466
Number of Samples:4 / 3
Experimental Purkinje cells study 1 (P21)
Purkinje cells were isolated from CB6-Tg(Gad1-EGFP)G42Zjh/J mouse strain. These cells were manually sorted from the cerebellum of a 21-days-old mouse study 1 (P21) based on EGFP fluorescence, shape, size and localization. The numbers of cells were from 50 to 75.
Control Purkinje cells study 1 (P3)
Purkinje cells were isolated from CB6-Tg(Gad1-EGFP)G42Zjh/J mouse strain. These cells were manually sorted from the cerebellum of a 3-days-old mouse (P3) based on EGFP fluorescence, shape, size and localization. The numbers of cells were from 50 to 75.

actinomycin D study 2 (late) / FBS stimulated fibroblast sample (129/B6)

Relative Expression (log2-ratio):-1.7726622
Number of Samples:5 / 5
Experimental actinomycin D study 2 (late)
129/B6 wild type fibroblasts were serum deprived for 16h. The cells were then stimulated by 10% FBS for 90 min after which 5ug/ml actinomycin D was added. Samples for RNA analysis were then removed at 120 minutes after actinomycin D treatment. ATC code:
Control FBS stimulated fibroblast sample (129/B6)
129/B6 wild type fibroblasts were serum deprived for 16h. The cells were then stimulated by 10% FBS for 90 min and harvested for RNA isolation.

Purkinje cells study 1 (P28) / Purkinje cells study 1 (P3)

Relative Expression (log2-ratio):-1.6687584
Number of Samples:3 / 3
Experimental Purkinje cells study 1 (P28)
Purkinje cells were isolated from CB6-Tg(Gad1-EGFP)G42Zjh/J mouse strain. These cells were manually sorted from the cerebellum of a 28-days-old mouse study 1 (P28) based on EGFP fluorescence, shape, size and localization. The numbers of cells were from 50 to 75.
Control Purkinje cells study 1 (P3)
Purkinje cells were isolated from CB6-Tg(Gad1-EGFP)G42Zjh/J mouse strain. These cells were manually sorted from the cerebellum of a 3-days-old mouse (P3) based on EGFP fluorescence, shape, size and localization. The numbers of cells were from 50 to 75.

Y. enterocolitica; IL-12; IL-18 study 1 / IL-12; IL-18 study 1

Relative Expression (log2-ratio):-1.5849895
Number of Samples:3 / 3
Experimental Y. enterocolitica; IL-12; IL-18 study 1
Splenic natural killer cells grown on IL-2 in vitro were treated will IL-12 and IL-18 and infected with Y. enterocolitica WA(pYV).
Control IL-12; IL-18 study 1
Splenic natural killer cells grown on IL-2 in vitro were treated will IL-12 and IL-18.