TOP TEN perturbations for Q8K448 (Mus musculus)

Organism: Mus musculus
Gene: Q8K448
Selected probe(set): 1434474_at
Platform: Affymetrix Mouse Genome 430 2.0 Array

Expression of Q8K448 (1434474_at) across 2635 perturbations tested by GENEVESTIGATOR:

dendritic cell study 3 (plasmocytoid DC) / dendritic cell study 3 (conventional DC)

Relative Expression (log2-ratio):2.8274403
Number of Samples:2 / 4
Experimental dendritic cell study 3 (plasmocytoid DC)
Splenic plasmocytoid dendritic cells isolated from adult wild type mice of C57BL/6 genetic background.
Control dendritic cell study 3 (conventional DC)
Conventional splenic dendritic cells isolated from adult wild type mice of C57BL/6 genetic background.

1-fluoro-2,4 dinitrobenzene study 1 (Cnr1-/-/Cnr2-/-) / vehicle treated ear tissue (Cnr1-/-/Cnr2-/-)

Relative Expression (log2-ratio):-2.7901182
Number of Samples:3 / 3
Experimental 1-fluoro-2,4 dinitrobenzene study 1 (Cnr1-/-/Cnr2-/-)
Cnr1-/-/Cnr2-/- mice without any skin lesions and ear tags were sensitized by painting 50 ul of 0.2% DNFB on the shaved abdomen on two consecutive days. For elicitation of CHS, right ears of mice were painted with 10 ul of 0.3% DNFB on day 5. Whole ear tissue was harvested 48h after the 2nd DNFB-challenge and immediately snap frozen in liquid nitrogen. ATC code:---
Control vehicle treated ear tissue (Cnr1-/-/Cnr2-/-)
Left ears of Cnr1-/-/Cnr2-/- mice, painted with 50 μl of acetone-olive oil on the shaved abdomen on two consecutive days.

1-fluoro-2,4 dinitrobenzene study 1 (C57BL/6J) / vehicle treated ear tissue (C57BL/6J)

Relative Expression (log2-ratio):-2.6436424
Number of Samples:3 / 3
Experimental 1-fluoro-2,4 dinitrobenzene study 1 (C57BL/6J)
C57BL/6J mice without any skin lesions and ear tags were sensitized by painting 50 ul of 0.2% DNFB on the shaved abdomen on two consecutive days. For elicitation of CHS, right ears of mice were painted with 10 ul of 0.3% DNFB on day 5. Whole ear tissue was harvested 48h after the 2nd DNFB-challenge and immediately snap frozen in liquid nitrogen. ATC code:---
Control vehicle treated ear tissue (C57BL/6J)
Left ears of C57BL/6J control mice, painted with 50 μl of acetone-olive oil on the shaved abdomen on two consecutive days.

RhoA knockout study 1 (in vivo) / RhoA knockout study 1 (in vitro)

Relative Expression (log2-ratio):2.541482
Number of Samples:2 / 3
Experimental RhoA knockout study 1 (in vivo)
Freshly isolated RhoA-null keratinocytes obtained from epidermis of RhoA (fl/fl) K5 Cre mice. Skin samples were washed for 5 min in PBS containing antibiotics followed by digestion with 0.8% trypsin in PBS (50 min; 37 °C). Obtained epidermis was shaken for 30 min in low-calcium MEM supplemented with 0.25 mg/ml DNAase I and 8 % FCS treated with Chelex. Cell suspension was filtered (70 um) and collected keratinocytes were washed once with DNase I medium, resuspended and analyzed. RhoA: Ras homolog gene family, member A.
Control RhoA knockout study 1 (in vitro)
Cultured primary RhoA-null keratinocytes obtained from epidermis of RhoA (fl/fl) K5 Cre mice. Primary keratinocytes were cultured in low-calcium MEM containing 5 ug/ml insulin, 10 ng/ml epidermal growth factor, 10 ug/ml transferrin, 10 uM phosphoethanolamine, 10 uM ethanolamine, 0.36 ug/ml hydrocortisone, 2 mM L-glutamine, 100 U/ml penicillin, 100 ug/ml streptomycin and 8% Chelex-treated FCS. RhoA: Ras homolog gene family, member A.

B6.129P2-Cebpgtm1Aki / B6.Cg-Atf4tm1Tow

Relative Expression (log2-ratio):-2.4238634
Number of Samples:3 / 3
Experimental B6.129P2-Cebpgtm1Aki
A mutant mouse strain has an exon 2, encoding the DNA binding and leucine zipper domains, disrupted by the insertion of a neomycin selection cassette. RT-PCR analysis showed a lack of transcript in total RNA extracts from the spleens and livers of homozygous mutant mice. Synonyms:Cebpg-/- Cebpgtm1Aki MGI:
Control B6.Cg-Atf4tm1Tow
In this mutant strain, a neomycin phosphotransferase resistance (neo) cassette replaces the entire coding region of the endogenous mouse activating transcription factor 4 (Atf4) gene, abolishing gene function. These mice may be useful for studying cell proliferation defects associated with blindness, osteoporosis, and stress responses. Synonyms:Atf4-/- Atf4tm1Tow MGI:

M. tuberculosis study 1 (TNF-alpha -/-; inf.; saline) / M. tuberculosis study 1 (TNF-alpha -/-; uninf.; saline)

Relative Expression (log2-ratio):-2.377921
Number of Samples:4 / 3
Experimental M. tuberculosis study 1 (TNF-alpha -/-; inf.; saline)
Lung samples obtained from M. tuberculosis H37Rv infected TNF-alpha -/- mouse strain on a mock (saline) treatment. One day before the infection with M. tuberculosis, mice (age at 8 - 12 weeks, female) were treated with saline. Subsequent saline treatment was applied in weekly intervals into infected mice. Pulmonary infection with M. tuberculosis H37Rv was performed under xylazine-ketamine anesthesia by delivering 20 ul of bacterial inoculum (1000±300 CFU) into each nostril. Twenty four hours post infection, the bacterial dose was confirmed in the lungs of 2 wild-type control mice. The experiment was terminated 1 month after M. tuberculosis inoculation and lung samples were processed for microarray analysis.
Control M. tuberculosis study 1 (TNF-alpha -/-; uninf.; saline)
Lung samples from non-infected TNF-alpha -/- mouse strain obtained after 1 month of mock (saline) treatment in weekly intervals.

CNS cell type study 8 (P25) / CNS cell type study 8 (P7)

Relative Expression (log2-ratio):2.2958202
Number of Samples:3 / 3
Experimental CNS cell type study 8 (P25)
Somatosensory cortex (S1) fast spiking interneurons were isolated from CB6-Tg(Gad1-EGFP)G42Zjh/J mouse strain. These cells were manually sorted from the cortex of 25-days-old mice based on EGFP fluorescence, shape, size and localization. The numbers of cells were from 48 to 55.
Control CNS cell type study 8 (P7)
Somatosensory cortex (S1) fast spiking interneurons were isolated from CB6-Tg(Gad1-EGFP)G42Zjh/J mouse strain. These cells were manually sorted from the cortex of 7-days-old mice based on EGFP fluorescence, shape, size and localization. The numbers of cells were from 52 to 85.

skin inflammation study 1 (IL-23; PBS) / normal dorsal skin tissue (C57BL/6J)

Relative Expression (log2-ratio):-2.2329512
Number of Samples:5 / 5
Experimental skin inflammation study 1 (IL-23; PBS)
Samples of mock treated psoriasis-like skin lesions induced by intradermal injection of IL-23. Skin inflammation was induced in seven weeks old C57BL/6 mice by intradermal injection of recombinant murine IL-23 (3ug in 100ul PBS) applied for four consecutive days (day 1-4). Mice were further mock treated subcutaneously with 100ul of PBS on day 4, 5 and 6. Samples were taken at day 7.
Control normal dorsal skin tissue (C57BL/6J)
Samples of dorsal skin tissue taken from 8 weeks old C57BL/6J mice.

amino acids deprivation study 1 (Cebpg-/-) / amino acids deprivation study 1 (Atf4-/-)

Relative Expression (log2-ratio):-2.2289782
Number of Samples:3 / 3
Experimental amino acids deprivation study 1 (Cebpg-/-)
Embryonic fibroblasts isolated from E13.5 Cebpg-/- mice and cultivated in normal DMEM–10% FBS with no additional supplementation. Medium was refreshed 24 h later and again at 48 h with amino acid deprivation medium for 3 hours. Cebpg-/- mouse embryonic fibroblasts (MEFs) proliferate poorly and exhibit oxidative stress due to reduced glutathione levels and impaired expression of several glutathione biosynthesis pathway genes.
Control amino acids deprivation study 1 (Atf4-/-)
Embryonic fibroblasts isolated from E13.5 Atf4-/- mice and cultivated in normal DMEM–10% FBS with no additional supplementation. Medium was refreshed 24 h later and again at 48 h with amino acid deprivation medium for 3 hours. Atf4-/- mouse embryonic fibroblasts exhibit impaired oxidative homeostasis due to reduced GSH pools and increased levels of ROS.

nonalcoholic steatohepatitis study 10 (MCD) / normal liver tissue (C57BL/6)

Relative Expression (log2-ratio):2.1940508
Number of Samples:4 / 4
Experimental nonalcoholic steatohepatitis study 10 (MCD)
Liver tissue samples obtained from C57BL/6 mice which developed nonalcoholic steatohepatitis (NASH). NASH was induced by feeding 8 weeks old mice with high fat (HF) diet which was methionine- and choline-deficient (MCD) (Oriental Yeast Co., Tokyo, Japan).
Control normal liver tissue (C57BL/6)
Liver tissue samples of C57BL/6 mice fed a regular chow diet for 8 weeks.