TOP TEN perturbations for Q9CRA7 (Mus musculus)

Organism: Mus musculus
Gene: Q9CRA7
Selected probe(set): 1417970_at
Platform: Affymetrix Mouse Genome 430 2.0 Array

Expression of Q9CRA7 (1417970_at) across 2552 perturbations tested by GENEVESTIGATOR:

lipopolysaccharide study 6 (LPS/mock) / lipopolysaccharide study 6 (mock, LPS)

Relative Expression (log2-ratio):1.8413353
Number of Samples:3 / 3
Experimental lipopolysaccharide study 6 (LPS/mock)
Macrophages were pretreated with 100 ng/ml lipopolysaccharide (LPS) for 16h. After 2h rest, cells were mock treated with control medium. Total RNA was prepared from macrophages 3h after mock treatment. ATC code:---
Control lipopolysaccharide study 6 (mock, LPS)
Macrophages mock treated with control medium for 16h. After 2h rest, cells were treated with 100 ng/ml lipopolysaccharide (LPS). Total RNA was prepared from macrophages 3h after LPS stimulation. ATC code:---

neuron culture dev. study 1 (late) / untreated hippocampus neuron cell sample (C57BL/6)

Relative Expression (log2-ratio):1.8382368
Number of Samples:6 / 3
Experimental neuron culture dev. study 1 (late)
Hippocampus neuron cells derived from hippocampi dissected from E17.5 C57BL/6 C-/C-mouse embryos at 12 or 16 days after plating (12 or 16 days in vitro).
Control untreated hippocampus neuron cell sample (C57BL/6)
Hippocampus neuron cells derived from hippocampi dissected from E17.5 C57BL/6 mouse embryos at 6 hours after plating (0.25 days in vitro).

lipopolysaccharide study 6 (mock, LPS) / untreated macrophage sample (C57BL/6) 2

Relative Expression (log2-ratio):-1.836092
Number of Samples:3 / 3
Experimental lipopolysaccharide study 6 (mock, LPS)
Macrophages mock treated with control medium for 16h. After 2h rest, cells were treated with 100 ng/ml lipopolysaccharide (LPS). Total RNA was prepared from macrophages 3h after LPS stimulation. ATC code:---
Control untreated macrophage sample (C57BL/6) 2
Macrophages untreated.

retina development study 2 (Nrl-EGFP; P21) / retina development study 2 (Nrl-EGFP; P6)

Relative Expression (log2-ratio):-1.4276075
Number of Samples:4 / 4
Experimental retina development study 2 (Nrl-EGFP; P21)
Retinal rod cells isolated at postnatal day 21 from Nrl-EGFP mouse strain. Mice were sacrificed by decapitation 2–3 hours subsequent to light onset. Retinas from 4–5 independent biological replicates were immediately dissected from enucleated eyes, and dissociated with trypsin. Reactions were quenched with SBTI (1 mg/ml), and GFP + cells were collected using a FACS. Total RNA was purified from pelleted cells (typically ,0.56106 GFP + events per retina).
Control retina development study 2 (Nrl-EGFP; P6)
Retinal rod cells isolated at postnatal day 6 from Nrl-EGFP mouse strain. Mice were sacrificed by decapitation 2–3 hours subsequent to light onset. Retinas from 4–5 independent biological replicates were immediately dissected from enucleated eyes, and dissociated with trypsin. Reactions were quenched with SBTI (1 mg/ml), and GFP + cells were collected using a FACS. Total RNA was purified from pelleted cells (typically ,0.56106 GFP + events per retina).

neuron culture dev. study 1 (intermediate) / untreated hippocampus neuron cell sample (C57BL/6)

Relative Expression (log2-ratio):1.3981848
Number of Samples:6 / 3
Experimental neuron culture dev. study 1 (intermediate)
Hippocampus neuron cells derived from hippocampi dissected from E17.5 C57BL/6 C-/C-mouse embryos at 4 or 8 days after plating (4 or 8 days in vitro).
Control untreated hippocampus neuron cell sample (C57BL/6)
Hippocampus neuron cells derived from hippocampi dissected from E17.5 C57BL/6 mouse embryos at 6 hours after plating (0.25 days in vitro).

burn injury study 2 (1d; splenocyte) / sham treated splenocyte sample (C57BL/6J; 1d)

Relative Expression (log2-ratio):-1.3950548
Number of Samples:4 / 4
Experimental burn injury study 2 (1d; splenocyte)
Splenocytes isolated from wild type males of C57BL/6J genetic background at 1st day after burn injury.
Control sham treated splenocyte sample (C57BL/6J; 1d)
Splenocytes at 1st day after sham burn injury.

lipopolysaccharide study 6 (LPS/LPS) / lipopolysaccharide study 6 (mock, LPS)

Relative Expression (log2-ratio):1.372673
Number of Samples:2 / 3
Experimental lipopolysaccharide study 6 (LPS/LPS)
Macrophages were pretreated with 100 ng/ml lipopolysaccharide (LPS) for 16h. After 2h rest, cells were re-stimulated with 100 ng/ml LPS. Total RNA was prepared from macrophages 3h after the second stimulation. ATC code:---
Control lipopolysaccharide study 6 (mock, LPS)
Macrophages mock treated with control medium for 16h. After 2h rest, cells were treated with 100 ng/ml lipopolysaccharide (LPS). Total RNA was prepared from macrophages 3h after LPS stimulation. ATC code:---

neuron culture dev. study 1 (late) / neuron culture dev. study 1 (early)

Relative Expression (log2-ratio):1.2808552
Number of Samples:6 / 6
Experimental neuron culture dev. study 1 (late)
Hippocampus neuron cells derived from hippocampi dissected from E17.5 C57BL/6 C-/C-mouse embryos at 12 or 16 days after plating (12 or 16 days in vitro).
Control neuron culture dev. study 1 (early)
Hippocampus neuron cells derived from hippocampi dissected from E17.5 C57BL/6 C-/C-mouse embryos at 1 or 2 days after plating (1 or 2 days in vitro).

mesenchymal progenitor cell differentiation study 1 (6d) / mesenchymal progenitor cell differentiation study 1 (0d)

Relative Expression (log2-ratio):1.2360201
Number of Samples:3 / 3
Experimental mesenchymal progenitor cell differentiation study 1 (6d)
C2C12 myoblasts stably expressing GFP (C2C12-pMirn0) differentiated for 6 days in the absence of recombinant human bone morphogenetic protein 2 (BMP2). Cells were firstly plated at 2.5x10e4 cells/cm2 in growing medium (DMEM, 10% newborn calf serum, 2 mM L-glutamine, antibiotics) and let grow for 24 hours. Then medium was replaced for differentiating medium (DMEM containing 5% newborn calf serum) and cells were further cultivated for 6 days. C2C12-GFP generation: cells were infected with lentiviruses containing pCDH-CMV-MCS-EF1-copGFP vector (System Biosciences) and resulting copGFP expressing cells were FACS sorted 48 hours later.
Control mesenchymal progenitor cell differentiation study 1 (0d)
C2C12 myoblasts stably expressing GFP (C2C12-pMirn0) taken at the beginning of differentiation (day 0). C2C12-GFP generation: cells were infected with lentiviruses containing pCDH-CMV-MCS-EF1-copGFP vector (System Biosciences) and resulting copGFP expressing cells were FACS sorted 48 hours later.

placenta development study 1 (fetal placenta; E19) / placenta development study 1 (fetal placenta; E9)

Relative Expression (log2-ratio):1.2228956
Number of Samples:2 / 3
Experimental placenta development study 1 (fetal placenta; E19)
Placenta tissue (fetal part/origin) isolated from wild type embryos (Swiss Webster) at E19 developmental stage.
Control placenta development study 1 (fetal placenta; E9)
Placenta tissue (fetal part/origin) isolated from wild type embryos (Swiss Webster) at E9 developmental stage.