TOP TEN perturbations for Q0E3C3 (Oryza sativa)

Organism: Oryza sativa
Gene: Q0E3C3
Selected probe(set): Os.1983.1.S1_at
Platform: Affymetrix Rice Genome Array

Expression of Q0E3C3 (Os.1983.1.S1_at) across 1064 perturbations tested by GENEVESTIGATOR:

drought (leaf; panicle elongation) / untreated leaf samples (panicle elongation)

Relative Expression (log2-ratio):-1.0897083
Number of Samples:3 / 3
Experimental drought (leaf; panicle elongation)
Leaf samples of line DK151 grown till panicle elongation stage in PVC tubes (75cm x 20cm, 20cm hole at the bottom; filled with turface) without water limitation (watered with 0.5x Yoshida nutrient solution) and then drought-stressed by slowly draining the solution until leaf relative water content (RWC) dropped to 65%-75% (about wilting). The nutrient solution as described in (Yoshida et al., 1976, Laboratory Manual for Physiological Studies of Rice, Ed 3, International Rice Research Institute, Manila, Philippines).
Control untreated leaf samples (panicle elongation)
Leaf samples of line DK151 grown till panicle elongation stage in PVC tubes (75cm x 20cm, 20cm hole at the bottom; filled with turface) without water limitation (watered with 0.5x Yoshida nutrient solution).

drought (Nagina 22) / untreated plant samples (Nagina 22)

Relative Expression (log2-ratio):-1.0247345
Number of Samples:3 / 3
Experimental drought (Nagina 22)
Whole plant samples of landrace Nagina 22 grown for 14 days on sterile absorbent cotton soaked with Hoagland’s solution (no water limitation) and then drought-stressed by withholding water till visible leaf rolling appeared (relative water content 64.8%). Other growth conditions: 14h light (Philips TL 40 W/54 tubes, 100-125 μmol photons m-2 s-1) / 10h dark cycles; 28 ± 1°C.
Control untreated plant samples (Nagina 22)
Whole plant samples of cultivar Nagina 22 grown for 14 days on sterile absorbent cotton soaked with Hoagland’s solution (no water limitation, relative water content 96%) under 14h light (Philips TL 40 W/54 tubes, 100-125 μmol photons m-2 s-1) / 10h dark cycles; 28 ± 1°C.

cold (LTH; 4°C for 48h; 29°C for 24h) / cold (LTH; 4°C for 48h)

Relative Expression (log2-ratio):-0.9728794
Number of Samples:3 / 3
Experimental cold (LTH; 4°C for 48h; 29°C for 24h)
Leaf samples of landrace Li-Jiang-Xin-Tuan-Hei-Gu (LTH) grown till the 3-leaf-stage on soil in a growth chamber under 12h light / 12h dark cycles at 29°C, then placed to 4°C for 48h, and then transferred back to 29°C for 24h.
Control cold (LTH; 4°C for 48h)
Leaf samples of landrace Li-Jiang-Xin-Tuan-Hei-Gu (LTH) grown till the 3-leaf-stage on soil in a growth chamber under 12h light / 12h dark cycles at 29°C, then placed to 4°C for 48h.

cold (IR29; 4°C for 48h; 29°C for 24h) / untreated leaf samples (IR29)

Relative Expression (log2-ratio):-0.96061707
Number of Samples:3 / 3
Experimental cold (IR29; 4°C for 48h; 29°C for 24h)
Leaf samples of cultivar IR29 grown till the 3-leaf-stage on soil in a growth chamber under 12h light / 12h dark cycles at 29°C, then placed to 4°C for 48h, and then transferred back to 29°C for 24h.
Control untreated leaf samples (IR29)
Leaf samples of cultivar IR29 grown till the 3-leaf-stage on soil in a growth chamber under 12h light / 12h dark cycles at 29°C, then left under the same conditions for 2h, 8h, 24h, 48h, and 72h.

anther development (tricellular pollen) / anther development (bicellular pollen)

Relative Expression (log2-ratio):0.90816593
Number of Samples:3 / 3
Experimental anther development (tricellular pollen)
Anthers with tricellular mature pollen were sampled from Nipponbare plants grown in a paddy field (Mishima, Japan) in May - September 2006.
Control anther development (bicellular pollen)
Anthers with bicellular pollen were sampled from Nipponbare plants grown in a paddy field (Mishima, Japan) in May - September 2006.

seed deterioration (45°C) / seed deterioration (40°C)

Relative Expression (log2-ratio):-0.8664255
Number of Samples:3 / 3
Experimental seed deterioration (45°C)
Embryo samples isolated from dry Nipponbare seeds that were first kept for 3 days at 25°C and 85% relative humidity (RH), then for 14 days at 45°C and 85% RH, and then for 3 days at 25°C and 32% RH.
Control seed deterioration (40°C)
Embryo samples isolated from dry Nipponbare seeds that were first kept for 3 days at 25°C and 85% relative humidity (RH), then for 14 days at 40°C and 85% RH, and then for 3 days at 25°C and 32% RH.

pollen development (tricellular immature) / pollen development (bicellular)

Relative Expression (log2-ratio):0.84351635
Number of Samples:3 / 3
Experimental pollen development (tricellular immature)
Immature tricellular pollen were sampled from Zhonghua 10 plants grown in a field at Beijing, China under natural conditions (May - September).
Control pollen development (bicellular)
Immature bicellular pollen were sampled from Zhonghua 10 plants grown in a field at Beijing, China under natural conditions (May - September).

Zhenshan 97 / MH63

Relative Expression (log2-ratio):-0.7992325
Number of Samples:2 / 2
Experimental Zhenshan 97
Indica (Oryza sativa ssp. indica) variety from Huazhong Agricultural University, Wuhan, China. Under the name Zhenshan 97, there exist at least two lines: Zhenshan 97B, the maintainer line for a number of elite hybrids, and the male sterile Zhenshan 97A line that has been used as a female parent for production of a popular hybrid line Shanyou 63, other parent of which is Minghui 63. Zhenshan 97 and Minghui 63 have also been used as parents to genetically map genes and QTLs. Zhenshan 97 (A and B) has poor cooking and eating quality due to low gelatinization temperature, high amylose content, hard gel consistency, and a chalky endosperm (Zhou et al., 2003,Theor Appl Genet. 106: 326–331). Zhenshan 97 is considered to be drought susceptible (Ji et al., 2011, J Plant Physiol.). Zhenshan 97 is not well transformable with Agrobacterium tumefaciens: using A.tumefaciens strain EHA 105, transient transformation frequency has been found to be 41.2%, stable transformation frequency only 2.21% (Tie et al., 2012, Plant Mol. Biol. 78: 1-18).
Control MH63
Minghui 63, indica cultivar.

cold (IR29; 4°C for 48h; 29°C for 24h) / cold (IR29; 4°C for 48h)

Relative Expression (log2-ratio):-0.7986908
Number of Samples:3 / 3
Experimental cold (IR29; 4°C for 48h; 29°C for 24h)
Leaf samples of cultivar IR29 grown till the 3-leaf-stage on soil in a growth chamber under 12h light / 12h dark cycles at 29°C, then placed to 4°C for 48h, and then transferred back to 29°C for 24h.
Control cold (IR29; 4°C for 48h)
Leaf samples of cultivar IR29 grown till the 3-leaf-stage on soil in a growth chamber under 12h light / 12h dark cycles at 29°C, then placed to 4°C for 48h.

anther development (tricellular pollen) / anther development (microspores)

Relative Expression (log2-ratio):-0.788455
Number of Samples:3 / 3
Experimental anther development (tricellular pollen)
Anthers with mature tricellular pollen were sampled from IR64 plants grown in a field (temperature range 25°C - 40°C, constant water supply).
Control anther development (microspores)
Anthers with unicellular microspores were sampled from IR64 plants grown in a field (temperature range 25°C - 40°C, constant water supply).