TOP TEN perturbations for Q68FS3 (Rattus norvegicus)

Organism: Rattus norvegicus
Gene: Q68FS3
Selected probe(set): 1399133_at
Platform: Affymetrix Rat Genome 230 2.0 Array

Expression of Q68FS3 (1399133_at) across 7639 perturbations tested by GENEVESTIGATOR:

spinal cord injury study 2 (60d) / sham-operated sacral spinal cord motor neuron cell sample (60d; Wistar)

Relative Expression (log2-ratio):1.3312283
Number of Samples:8 / 5
Experimental spinal cord injury study 2 (60d)
Motor neurons isolated from the sacral segment of the spinal cord (S3-S4 level) from male Wistar rats (325 - 480 g) 60 days after spinalization. Before operation the rats were initially anesthetized with isoflurane and thereafter a xylocaine–marcaine mix (xylocaine, 12.5 g/ ml:marcaine, 2.5 mg/ml, 1:4) of 0.2–0.3 ml per animal was injected intramuscularly. The second, and a few times also the third, lumbar vertebrae (L2 and L3) were removed. The dura was opened and spinal cord tissue corresponding to 1–2 mm was gently removed using a glass rod, forceps and suction—creating a clear separation between the rostral and caudal cord at the sacral S2 segment. Only rats in which the dorsal vein and the ventral artery remained intact across the dissected gap were included in the study. After spinalization, the wound was closed suturing muscles, muscle fascia, and skin separately. Care was taken to relieve pain postoperatively by administration of buprenorfin subcutaneously. Motor neurons were retrogradely labeled by injection of Fluoro-Gold dissolved in saline into both the tail muscles (150 ul) and intraperitoneally (30 ul). All animals were injected with this dual-injection procedure under anesthesia (isoflurane) 5 to 7 days before the spinal cord was removed. The isolated and frozen spinal cords (the S3–S4 level), where most of the tail motor neurons are located were cryosectioned into 10 um sections. The Fluoro-Gold–labeled motor neurons were isolated from the spinal cord sections using the Leica AS laser microdissection system at room temperature. Between 70 and 200 Fluoro-Gold–labeled neurons were laser microdissected per rat and the motor neurons were collected in the cap of a Eppendorf tube by force of gravity. RNA degradation and contamination from external sources were minimized in all steps of the experimental procedure.
Control sham-operated sacral spinal cord motor neuron cell sample (60d; Wistar)
Motor neurons isolated from the sacral segment of the spinal cord of the sham-operated male Wistar rats (325 - 480 g) 60 days after the operation. Before operation the rats were initially anesthetized with isoflurane and thereafter a xylocaine–marcaine mix (xylocaine, 12.5 g/ ml:marcaine, 2.5 mg/ml, 1:4) of 0.2–0.3 ml per animal was injected intramuscularly. The second, and a few times also the third, lumbar vertebrae (L2 and L3) were removed. The dura was left intact after laminectomy and the wound was closed suturing muscles, muscle fascia, and skin separately. Motor neurons were retrogradely labeled by injection of Fluoro-Gold dissolved in saline into both the tail muscles (150 ul) and intraperitoneally (30 ul). All animals were injected with this dual-injection procedure under anesthesia (isoflurane) 5 to 7 days before the spinal cord was removed. The isolated and frozen spinal cords (the S3–S4 level, where most of the tail motor neurons are located were cryosectioned into 10 um sections. The Fluoro-Gold–labeled motor neurons were isolated from the spinal cord sections using the Leica AS laser microdissection system at room temperature. Between 70 and 200 Fluoro-Gold–labeled neurons were laser microdissected per rat and the motor neurons were collected in the cap of a Eppendorf tube by force of gravity. RNA degradation and contamination from external sources were minimized in all steps of the experimental procedure.

neuropathic pain study 1 (L5; ipsilateral) / neuropathic pain study 1 (L4; ipsilateral)

Relative Expression (log2-ratio):1.2668447
Number of Samples:5 / 5
Experimental neuropathic pain study 1 (L5; ipsilateral)
Ipsilateral L5 dorsal root ganglion (DRG) tissue isolated from rats 4 weeks after a L5 spinal nerve ligation (SNL). Male Sprague-Dawley rats weighing 150-174 g were used in the study. SNL was used as a model of neuropathic pain, in which L5 spinal nerve was isolated and tightly ligated distal to the dorsal root ganglia with a 4-0 silk suture. Ipsilateral L5 DRGs were excised 4 weeks after the surgery under ketamine HCl anesthesia. Postoperatively, rats were housed individually under the same general conditions.
Control neuropathic pain study 1 (L4; ipsilateral)
Ipsilateral L4 dorsal root ganglion (DRG) tissue isolated from rats 4 weeks after a L5 spinal nerve ligation (SNL). Male Sprague-Dawley rats weighing 150-174 g were used in the study. SNL was used as a model of neuropathic pain, in which L5 spinal nerve was isolated and tightly ligated distal to the dorsal root ganglia with a 4-0 silk suture. Ipsilateral L4 DRGs were excised 4 weeks after the surgery under ketamine HCl anesthesia. Postoperatively, rats were housed individually under the same general conditions.

neuropathic pain study 1 (L4; contralateral) / control dorsal root ganglion tissue (Sprague Dawley; L4; contralateral)

Relative Expression (log2-ratio):-1.1205854
Number of Samples:5 / 5
Experimental neuropathic pain study 1 (L4; contralateral)
Contralateral L4 dorsal root ganglion (DRG) tissue isolated from rats 4 weeks after a L5 spinal nerve ligation (SNL). Male Sprague-Dawley rats weighing 150-174 g were used in the study. SNL was used as a model of neuropathic pain, in which L5 spinal nerve was isolated and tightly ligated distal to the dorsal root ganglia with a 4-0 silk suture. Contralateral L4 DRGs were excised 4 weeks after the surgery under ketamine HCl anesthesia. Postoperatively, rats were housed individually under the same general conditions.
Control control dorsal root ganglion tissue (Sprague Dawley; L4; contralateral)
Contralateral L4 dorsal root ganglion (DRG) tissue isolated from sham-operated rat. Male Sprague-Dawley rats weighing 150-174 g were used in the study. Sham operation with no L5 spinal nerve ligation was performed and postoperatively rats underwent the same handling as the experimental group. Contralateral L4 DRGs were excised 4 weeks after the surgery under ketamine HCl anesthesia.

phenobarbital study 10 (10000uM) / vehicle treated (medium) hepatocyte samples (Sprague Dawley)

Relative Expression (log2-ratio):-1.0651283
Number of Samples:2 / 2
Experimental phenobarbital study 10 (10000uM)
Hepatocyte samples derived from 6 weeks old male Sprague Dawley rats, treated with compound: phenobarbital (10000uM; CHEMBL40). Hepatocytes were treated for 2 hours. ATC code:
Control vehicle treated (medium) hepatocyte samples (Sprague Dawley)
Hepatocyte samples derived from 6 weeks old male Sprague Dawley rats, treated with vehicle (medium). Hepatocytes were vehicle treated for 2 hours.

neuropathic pain study 1 (L4; ipsilateral) / control dorsal root ganglion tissue (Sprague Dawley; L4; ipsilateral)

Relative Expression (log2-ratio):-1.0486422
Number of Samples:5 / 5
Experimental neuropathic pain study 1 (L4; ipsilateral)
Ipsilateral L4 dorsal root ganglion (DRG) tissue isolated from rats 4 weeks after a L5 spinal nerve ligation (SNL). Male Sprague-Dawley rats weighing 150-174 g were used in the study. SNL was used as a model of neuropathic pain, in which L5 spinal nerve was isolated and tightly ligated distal to the dorsal root ganglia with a 4-0 silk suture. Ipsilateral L4 DRGs were excised 4 weeks after the surgery under ketamine HCl anesthesia. Postoperatively, rats were housed individually under the same general conditions.
Control control dorsal root ganglion tissue (Sprague Dawley; L4; ipsilateral)
Ipsilateral L4 dorsal root ganglion (DRG) tissue isolated from sham-operated rat. Male Sprague-Dawley rats weighing 150-174 g were used in the study. Sham operation with no L5 spinal nerve ligation was performed and postoperatively rats underwent the same handling as the experimental group. Ipsilateral L4 DRGs were excised 4 weeks after the surgery under ketamine HCl anesthesia.

paracetamol (acetaminophen) study 18 (1000uM) / vehicle treated (medium) hepatocyte samples (Sprague Dawley)

Relative Expression (log2-ratio):-0.9811716
Number of Samples:2 / 2
Experimental paracetamol (acetaminophen) study 18 (1000uM)
Hepatocyte samples derived from 6 weeks old male Sprague Dawley rats, treated with compound: acetaminophen (1000uM; CHEMBL112). Hepatocytes were treated for 8 hours. ATC code:
Control vehicle treated (medium) hepatocyte samples (Sprague Dawley)
Hepatocyte samples derived from 6 weeks old male Sprague Dawley rats, treated with vehicle (medium). Hepatocytes were vehicle treated for 8 hours.

phenobarbital study 11 (1000uM) / vehicle treated (medium) hepatocyte samples (Sprague Dawley)

Relative Expression (log2-ratio):-0.9624834
Number of Samples:2 / 2
Experimental phenobarbital study 11 (1000uM)
Hepatocyte samples derived from 6 weeks old male Sprague Dawley rats, treated with compound: phenobarbital (1000uM; CHEMBL40). Hepatocytes were treated for 8 hours. ATC code:
Control vehicle treated (medium) hepatocyte samples (Sprague Dawley)
Hepatocyte samples derived from 6 weeks old male Sprague Dawley rats, treated with vehicle (medium). Hepatocytes were vehicle treated for 8 hours.

mifepristone study 6 (300 mg/kg) / vehicle treated (corn oil) liver tissue samples (Crj:CD(SD)IGS)

Relative Expression (log2-ratio):0.8817034
Number of Samples:3 / 18
Experimental mifepristone study 6 (300 mg/kg)
Liver samples from Crj:CD(SD)IGS rats treated with compound: mifepristone (300 mg/kg; vehicle: corn oil) for 1 day. ATC code:
Control vehicle treated (corn oil) liver tissue samples (Crj:CD(SD)IGS)
Liver samples derived from 6 weeks old male Crj:CD(SD)IGS rats, treated with vehicle (corn oil). Rats were sacrificed 1 day after the last treatment.

kidney development study 3 (P28) / kidney development study 3 (E13)

Relative Expression (log2-ratio):0.8572664
Number of Samples:3 / 3
Experimental kidney development study 3 (P28)
Kidney tissue isolated from rat (unspecified strain) at the postnatal week 4 (P28).
Control kidney development study 3 (E13)
Kidney tissue isolated from rat (unspecified strain) at the embryonic day 13.

dextran sulphate sodium study 1 (early) / untreated full-thickness colon tissue (Sprague Dawley)

Relative Expression (log2-ratio):-0.8339882
Number of Samples:12 / 6
Experimental dextran sulphate sodium study 1 (early)
Dextran sulphate sodium (DSS) induced colitis as model for inflammatory bowel disease: full-thickness colon tissue samples of female Sprague Dawley rats retrieving 5% DSS in drinking water from day 1 to 7. Samples were taken 1, 3, 5 and 7 days after application of DSS. ATC code:---
Control untreated full-thickness colon tissue (Sprague Dawley)
Full-thickness colon tissue samples of untreated female Sprague Dawley rats retrieving water only.