TOP TEN perturbations for Q9EQZ1 (Rattus norvegicus)

Organism: Rattus norvegicus
Gene: Q9EQZ1
Selected probe(set): 1367771_at
Platform: Affymetrix Rat Genome 230 2.0 Array

Expression of Q9EQZ1 (1367771_at) across 7639 perturbations tested by GENEVESTIGATOR:

caffeine study 18 (10000uM) / vehicle treated (medium) hepatocyte samples (Sprague Dawley)

Relative Expression (log2-ratio):2.7370796
Number of Samples:2 / 2
Experimental caffeine study 18 (10000uM)
Hepatocyte samples derived from 6 weeks old male Sprague Dawley rats, treated with compound: caffeine (10000uM; CHEMBL113). Hepatocytes were treated for 8 hours. ATC code:
Control vehicle treated (medium) hepatocyte samples (Sprague Dawley)
Hepatocyte samples derived from 6 weeks old male Sprague Dawley rats, treated with vehicle (medium). Hepatocytes were vehicle treated for 8 hours.

dexamethasone study 15 (392ug/ml) / vehicle treated (DME) bronchial epithelial cell sample (AGA)

Relative Expression (log2-ratio):2.1560497
Number of Samples:3 / 16
Experimental dexamethasone study 15 (392ug/ml)
Bronchial epithelial cells (NRBE) treated with dexamethasone (392ug/ml; vendor: Prestwick Chemical / catalog number: 130 / catalog name: Dexamethasone acetate [1177-87-3]) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NRBE cells were isolated from pooled tracheobronchial tissue of adult AGA rats. ATC code:, , , , , , , , , ,
Control vehicle treated (DME) bronchial epithelial cell sample (AGA)
Bronchial epithelial cells (NRBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NRBE cells were isolated from pooled tracheobronchial tissue of adult AGA rats.

naproxen study 10 (2000uM) / vehicle treated (medium) hepatocyte samples (Sprague Dawley)

Relative Expression (log2-ratio):2.1449795
Number of Samples:2 / 2
Experimental naproxen study 10 (2000uM)
Hepatocyte samples derived from 6 weeks old male Sprague Dawley rats, treated with compound: naproxen (2000uM; CHEMBL154). Hepatocytes were treated for 8 hours. ATC code:, ,
Control vehicle treated (medium) hepatocyte samples (Sprague Dawley)
Hepatocyte samples derived from 6 weeks old male Sprague Dawley rats, treated with vehicle (medium). Hepatocytes were vehicle treated for 8 hours.

lansoprazole (240 uM) / vehicle treated (DMSO) hepatocytes tissue samples (Crj:CD(SD)IGS)

Relative Expression (log2-ratio):-2.030136
Number of Samples:2 / 49
Experimental lansoprazole (240 uM)
Hepatocytes samples from Crj:CD(SD)IGS rats treated with compound: lansoprazole (240 uM; vehicle: DMSO) for 0.67 days. ATC code:
Control vehicle treated (DMSO) hepatocytes tissue samples (Crj:CD(SD)IGS)
Hepatocytes samples derived from 6 weeks old male Crj:CD(SD)IGS rats, treated with vehicle (DMSO). Rats were sacrificed 0.67 days after the last treatment.

astrocyte cultivation study 1 (P7; IP; HBEGF) / astrocyte cultivation study 1 (P7; IP; FCS)

Relative Expression (log2-ratio):-2.0212088
Number of Samples:3 / 3
Experimental astrocyte cultivation study 1 (P7; IP; HBEGF)
Astrocytes isolated from rat cerebral cortex at postnatal day 7 (P7) using immunopanning method (IP) and cultivated 1 week in vitro. Cortices from 6-10 postnatal Sprague Dawley rats were dissected and enzymatically dissociated to produce a single cell suspension. This suspension was then passing over the successive negative immunopanning plate to rid of microglia, endothelial cells, oligodendrocyte precursor cells and then passing the suspension over a positive selection plate with integrin beta 5 (Itgb5) antibody to select astrocytes. Astrocytes were cultivated for 7 days in vitro with heparin-binding epidermal growth factor (HBEGF). At day 7, the cells were processed for their total RNA.
Control astrocyte cultivation study 1 (P7; IP; FCS)
Astrocytes isolated from rat cerebral cortex at postnatal day 7 (P7) using immunopanning method (IP) and cultivated 1 week in vitro. Cortices from 6-10 postnatal Sprague Dawley rats were dissected and enzymatically dissociated to produce a single cell suspension. This suspension was then passing over the successive negative immunopanning plate to rid of microglia, endothelial cells, oligodendrocyte precursor cells and then passing the suspension over a positive selection plate with integrin beta 5 (Itgb5) antibody to select astrocytes. Astrocytes were cultivated for 7 days in vitro with fetal calf serum (FCS). At day 7, the cells were processed for their total RNA.

gentamicin study 20 (30uM) / vehicle treated (medium) hepatocyte samples (Sprague Dawley)

Relative Expression (log2-ratio):1.93361
Number of Samples:2 / 2
Experimental gentamicin study 20 (30uM)
Hepatocyte samples derived from 6 weeks old male Sprague Dawley rats, treated with compound: gentamicin (30uM; CHEMBL1201259). Hepatocytes were treated for 8 hours. ATC code:, , , ,
Control vehicle treated (medium) hepatocyte samples (Sprague Dawley)
Hepatocyte samples derived from 6 weeks old male Sprague Dawley rats, treated with vehicle (medium). Hepatocytes were vehicle treated for 8 hours.

captopril study 18 (10000uM) / vehicle treated (medium) hepatocyte samples (Sprague Dawley)

Relative Expression (log2-ratio):1.9004688
Number of Samples:2 / 2
Experimental captopril study 18 (10000uM)
Hepatocyte samples derived from 6 weeks old male Sprague Dawley rats, treated with compound: captopril (10000uM; CHEMBL1560). Hepatocytes were treated for 8 hours. ATC code:,
Control vehicle treated (medium) hepatocyte samples (Sprague Dawley)
Hepatocyte samples derived from 6 weeks old male Sprague Dawley rats, treated with vehicle (medium). Hepatocytes were vehicle treated for 8 hours.

indometacin study 4 (15mg/kg) / vehicle treated (0.5% methylcellulose) liver tissue samples (Crj:CD(SD)IGS)

Relative Expression (log2-ratio):-1.8959999
Number of Samples:3 / 3
Experimental indometacin study 4 (15mg/kg)
Liver samples derived from 6 weeks old male Crj:CD(SD)IGS rats, treated with compound: indometacin (15mg/kg; CHEMBL6). Rats were treated by gavage and sacrificed after 24 hours of treatment. ATC code:, , ,
Control vehicle treated (0.5% methylcellulose) liver tissue samples (Crj:CD(SD)IGS)
Liver samples derived from 6 weeks old male Crj:CD(SD)IGS rats, treated with vehicle (0.5% methylcellulose). Rats were treated by gavage and sacrificed after 24 hours.

pantoprazole (650 uM) / vehicle treated (DMSO) hepatocytes tissue samples (Crj:CD(SD)IGS)

Relative Expression (log2-ratio):-1.8553534
Number of Samples:3 / 49
Experimental pantoprazole (650 uM)
Hepatocytes samples from Crj:CD(SD)IGS rats treated with compound: pantoprazole (650 uM; vehicle: DMSO) for 0.67 days. ATC code:,
Control vehicle treated (DMSO) hepatocytes tissue samples (Crj:CD(SD)IGS)
Hepatocytes samples derived from 6 weeks old male Crj:CD(SD)IGS rats, treated with vehicle (DMSO). Rats were sacrificed 0.67 days after the last treatment.

synapse development study 2 (P21) / synapse development study 2 (P3)

Relative Expression (log2-ratio):1.8296757
Number of Samples:5 / 6
Experimental synapse development study 2 (P21)
Globular bushy cells (GBC) were isolated from 12 um thick slices of ventral cochlear nucleus using laser microdissection at postnatal day 21 (P21). GBCs were retrogradely labeled by stereotaxic injection of fluorescent (Alexa 488 or 596) cholera toxin-B (CTB) at postnatal day 20. Approximately 1.5 ul of CTB solution [1 mg/ml in phosphate-buffered saline (PBS)] was slowly injected into the contralateral medial nucleus of the trapezoid body nucleus. At this time point calyx is fully mature. 200 CTB-labeled cells collected from the same animal represent one sample.
Control synapse development study 2 (P3)
Globular bushy cells (GBC) were isolated from 12 um thick slices of the ventral cochlear nucleus using laser microdissection at postnatal day 3 (P3). GBCs were retrogradely labeled by stereotaxic injection of fluorescent (Alexa 488 or 596) cholera toxin-B (CTB) at postnatal day 2. Approximately 1.5 ul of CTB solution [1 mg/ml in phosphate-buffered saline (PBS)] was slowly injected into the contralateral medial nucleus of the trapezoid body nucleus. At this time point there is a calyx of Held formation. 200 CTB-labeled cells collected from the same animal represent one sample.