TOP TEN perturbations for Q711P4 (Sus scrofa)

Organism: Sus scrofa
Gene: Q711P4
Selected probe(set): Ssc.23797.1.S1_at
Platform: Affymetrix Porcine Genome Array

Expression of Q711P4 (Ssc.23797.1.S1_at) across 190 perturbations tested by GENEVESTIGATOR:

cell passage study 1 (P0) / unstimulated (P2) valvular interstitial cell sample (n.s.)

Relative Expression (log2-ratio):6.8208838
Number of Samples:3 / 3
Experimental cell passage study 1 (P0)
Freshly isolated primary valvular interstitial cells (VICs) without any treatment and without having been cultured.
Control unstimulated (P2) valvular interstitial cell sample (n.s.)
Primary valvular interstitial cells (VICs) cultured on plastic TCPS until passage 2 (P2). Cells were isolated from adult porcine aortic valves and cultured in media containing 1% fetal bovine serum. Samples were harvested 3 days after isolation.

lipopolysaccharide study 1 (2h) / unstimulated bone marrow-derived macrophage sample (LD x LW)

Relative Expression (log2-ratio):5.134779
Number of Samples:6 / 6
Experimental lipopolysaccharide study 1 (2h)
Bone marrow-derived macrophages (BMDM) isolated from 8-12 weeks old Landrace-Large White crossbreed pigs, stimulated for 2 hours with 100 ng/ml lipopolysaccharide (LPS) from Salmonella enterica. After isolation BMDMs were frozen overnight in freezing medium followed by 5-7 days differentiation in complete RPMI 1640 medium (10% heat-inactivated FCS) containing 10exp4 U/ml rhCSF. After differentiation 10exp6 BMDMs/ml were seeded in 6 wells plates and left overnight before stimulation. The LPS used in the experiment was from serotype minnesota Re 595 (Sigma-Aldrich L9764). After 2 hours LPS stimulation total RNA was isolated. ATCvet code:---
Control unstimulated bone marrow-derived macrophage sample (LD x LW)
Bone marrow-derived macrophages (BMDM) isolated from 8-12 weeks old Landrace-Large White crossbreed pigs. After isolation BMDMs were frozen overnight in freezing medium followed by 5-7 days differentiation in complete RPMI 1640 medium (10% heat-inactivated FCS) containing 10exp4 U/ml rhCSF. After differentiation 10exp6 BMDMs/ml were seeded in 6 wells plates and left overnight before total RNA isolation.

lipopolysaccharide study 1 (7h) / unstimulated bone marrow-derived macrophage sample (LD x LW)

Relative Expression (log2-ratio):4.2041225
Number of Samples:6 / 6
Experimental lipopolysaccharide study 1 (7h)
Bone marrow-derived macrophages (BMDM) isolated from 8-12 weeks old Landrace-Large White crossbreed pigs, stimulated for 7 hours with 100 ng/ml lipopolysaccharide (LPS) from Salmonella enterica. After isolation BMDMs were frozen overnight in freezing medium followed by 5-7 days differentiation in complete RPMI 1640 medium (10% heat-inactivated FCS) containing 10exp4 U/ml rhCSF. After differentiation 10exp6 BMDMs/ml were seeded in 6 wells plates and left overnight before stimulation. The LPS used in the experiment was from serotype minnesota Re 595 (Sigma-Aldrich L9764). After 7 hours LPS stimulation total RNA was isolated. ATCvet code:---
Control unstimulated bone marrow-derived macrophage sample (LD x LW)
Bone marrow-derived macrophages (BMDM) isolated from 8-12 weeks old Landrace-Large White crossbreed pigs. After isolation BMDMs were frozen overnight in freezing medium followed by 5-7 days differentiation in complete RPMI 1640 medium (10% heat-inactivated FCS) containing 10exp4 U/ml rhCSF. After differentiation 10exp6 BMDMs/ml were seeded in 6 wells plates and left overnight before total RNA isolation.

lipopolysaccharide study 1 (24h) / unstimulated bone marrow-derived macrophage sample (LD x LW)

Relative Expression (log2-ratio):2.4628096
Number of Samples:5 / 6
Experimental lipopolysaccharide study 1 (24h)
Bone marrow-derived macrophages (BMDM) isolated from 8-12 weeks old Landrace-Large White crossbreed pigs, stimulated for 24 hours with 100 ng/ml lipopolysaccharide (LPS) from Salmonella enterica. After isolation BMDMs were frozen overnight in freezing medium followed by 5-7 days differentiation in complete RPMI 1640 medium (10% heat-inactivated FCS) containing 10exp4 U/ml rhCSF. After differentiation 10exp6 BMDMs/ml were seeded in 6 wells plates and left overnight before stimulation. The LPS used in the experiment was from serotype minnesota Re 595 (Sigma-Aldrich L9764). After 24 hours LPS stimulation total RNA was isolated. ATCvet code:---
Control unstimulated bone marrow-derived macrophage sample (LD x LW)
Bone marrow-derived macrophages (BMDM) isolated from 8-12 weeks old Landrace-Large White crossbreed pigs. After isolation BMDMs were frozen overnight in freezing medium followed by 5-7 days differentiation in complete RPMI 1640 medium (10% heat-inactivated FCS) containing 10exp4 U/ml rhCSF. After differentiation 10exp6 BMDMs/ml were seeded in 6 wells plates and left overnight before total RNA isolation.

kidney transplantation study 1 (24h) / normal kidney tissue (YS)

Relative Expression (log2-ratio):2.4360762
Number of Samples:3 / 2
Experimental kidney transplantation study 1 (24h)
Transplanted kidney samples from Yorkshire pigs 24 hours after transplantation. Before organ removal from donor pig, aorta was cross-clamped proximally and distally for 60 minutes (warm ischemia time) to generate warm ischemia. After that time aorta was infused with 300 ml cold UW solution for 3-5 minutes. Kidney was then removed, washed with 300 ml UW solution and placed on ice for 24 hours (cold ischemia time). Donor kidney was then reperfused and transplanted into the allograft recipient pig. Recipient pigs were treated with tacrolimus (0.5-0.8 mg/kg) starting one day before surgery.
Control normal kidney tissue (YS)
Kidney samples from Yorkshire pigs.

diet study 11 (28 dpn) / diet study 11 (94 dpc)

Relative Expression (log2-ratio):2.3489313
Number of Samples:15 / 16
Experimental diet study 11 (28 dpn)
Liver samples of 28 days old German Landrace piglets under maternal low protein (LP) diet, containing 6% crude protein. The diet during pregnancy had an energetic content of 13.6 MJ ME/kg. After 115 days of gestation animals were subjected to prostaglandin induced parturition and offspring were fed for 28 days with adequate protein lactation diet by cross fostering. At 28 dpn piglets were fasted overnight and livers harvested. All male animals used in this experiments were castrated at 4 days of age.
Control diet study 11 (94 dpc)
Liver samples of 94 dpc German Landrace fetus under maternal low protein (LP) diet, containing 6% crude protein. The diet during pregnancy had an energetic content of 13.6 MJ ME/kg. After 94 days of gestation animals were subjected to caesarean section and liver samples of the fetus were harvested.

diet study 11 (28 dpn) / diet study 11 (1 dpn)

Relative Expression (log2-ratio):2.2026348
Number of Samples:15 / 14
Experimental diet study 11 (28 dpn)
Liver samples of 28 days old German Landrace piglets under maternal low protein (LP) diet, containing 6% crude protein. The diet during pregnancy had an energetic content of 13.6 MJ ME/kg. After 115 days of gestation animals were subjected to prostaglandin induced parturition and offspring were fed for 28 days with adequate protein lactation diet by cross fostering. At 28 dpn piglets were fasted overnight and livers harvested. All male animals used in this experiments were castrated at 4 days of age.
Control diet study 11 (1 dpn)
Liver samples of 1 day old German Landrace piglets under maternal low protein (LP) diet, containing 6% crude protein. The diet during pregnancy had an energetic content of 13.6 MJ ME/kg. After 115 days of gestation animals were subjected to prostaglandin induced parturition and the liver samples of 1 day old offsprings were harvested.

senescence study 1 / normal coronary arterial endothelial cell sample (n.s.)

Relative Expression (log2-ratio):-2.039095
Number of Samples:3 / 3
Experimental senescence study 1
1 week cultured porcine coronary arterial endothelial cell samples, on which serial passaging was performed on a weekly basis until passage four (senescent cells). Cells were isolated from female pigs between 3 and 4 months of age and weighting between 25 and 30 kg.
Control normal coronary arterial endothelial cell sample (n.s.)
1 week cultured coronary arterial endothelial cells, at passage one. Cells were isolated from female pigs between 3 and 4 months of age and weighting between 25 and 30 kg.

S. choleraesuis study 1 (48hpi) / uninfected mesenteric lymph node tissue (n.s.)

Relative Expression (log2-ratio):2.0202951
Number of Samples:3 / 3
Experimental S. choleraesuis study 1 (48hpi)
Mesenteric lymph node samples from 7 weeks old pigs, intranasally infected with 1 billion CFU of S. choleraesuis x3246 and necropsied 48h post infection. Animals were free of S. choleraesuis infection prior to experiment (checked two times by rectal culture).
Control uninfected mesenteric lymph node tissue (n.s.)
Mesenteric lymph node samples from 7 weeks old pigs, necropsied 3 days before start of experimental infection. Animals were free of S. choleraesuis infection (checked two times by rectal culture).

diet study 11 (188 dpn) / diet study 11 (94 dpc)

Relative Expression (log2-ratio):2.0071754
Number of Samples:15 / 16
Experimental diet study 11 (188 dpn)
Liver samples of 188 days old German Landrace piglets under maternal low protein (LP) diet, containing 6% crude protein. The diet during pregnancy had an energetic content of 13.6 MJ ME/kg. After 115 days of gestation animals were subjected to prostaglandin induced parturition and offspring were fed for 28 days with adequate protein lactation diet by cross fostering and further 160 days with standard diet ad libitum. At 188 dpn piglets were fasted overnight and livers harvested. All male animals used in this experiments were castrated at 4 days of age.
Control diet study 11 (94 dpc)
Liver samples of 94 dpc German Landrace fetus under maternal low protein (LP) diet, containing 6% crude protein. The diet during pregnancy had an energetic content of 13.6 MJ ME/kg. After 94 days of gestation animals were subjected to caesarean section and liver samples of the fetus were harvested.